38 results about "Providencia rettgeri" patented technology

The invention discloses an ammonia nitrogen-removed compound microorganism microbial agent. The ammonia nitrogen-removed compound microorganism microbial agent is prepared from Providencia rettgeri, Microbacterium lactium, Lactobacillus delbrueckii and Rhodococcus coprophilus in a volume ratio of 5 to 2.5 to 1 to 5. The invention further discloses application of the compound microorganism microbial agent in ammonia nitrogen-containing wastewater treatment. The four bacteria are reasonably matched and are combined by virtue of an adsorption method and an embedding method to form immobilized cell particles, so that the ammonia-nitrogen degradation capacity and stability of the compound microorganism microbial agent are remarkably enhanced; a dominant bacterial community can be immediately formed after the compound microorganism microbial agent is applied to denitrification and treatment process of sewage, river way landscape water and the like, the treatment time is short, and ammonia and nitrogen indexes of the sewage are rapidly decreased; and meanwhile, the compound microorganism microbial agent has an aerobic denitrification function and further has relatively good effect of removing COD, and accumulation of nitrite and nitrate is avoided.

The invention discloses a Providencia rettgeri strain and application thereof. The Providencia rettgeri YL is collected in China General Microbiological Culture Collection Center (CGMCC) on April 28th, 2013, and the collection number is CGMCC No.7544. The Providencia rettgeri is used for treating nitrogenous organic wastewater, and can effectively remove organic nitrogen, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen while removing organic carbon, and thus, the whole organic carbon and nitrogen synchronous removing process can be implemented only by one aerobic stage. The strain has the heterotrophic nitrification-aerobic denitrification function, can synchronously remove the organic carbon and nitrogen under aerobic conditions, can greatly reduce the occupied area and construction investment, can shorten the system start-up time, reduces the treatment cost, and enhances the treatment efficiency.

The invention discloses a root-knot nematode biocontrol bacterium, an antibiological inoculant and application of the biocontrol bacterium and the antibiological inoculant. The biocontrol bacterium is a Providencia rettgeri strain UN06, and the preservation serial number of the biocontrol bacterium is CGMCC No. 10246. Fermentation liquor obtained through fermentation of the Providencia rettgeri strain UN06, adsorbing carriers such as mushroom dreg and grass carbon, and nutrient substance urea required by bacterium growth are evenly mixed to be granulated to form UN06 granules. The control efficiencies of 100 kilograms of the applied UN06 granules in each mu to tomato root-knot eelworms, cucumber root-knot nematodes and tobacco root-knot nematodes are 90.17 percent, 88.66 percent and 85.98 percent. The root-knot nematode antibiological inoculant has the advantages of being low in cost, high in control efficiency, free of residual and safe to people and livestock, and is an efficient biopesticide for preventing and treating root knot nematode diseases.

The invention discloses a strain for degrading a pendimethalin pesticide and a fungicide and an application of the strain. The strain is Providencia rettgeri JZB42C003 which is preserved at CGMCC on 17th, November, 2014, wherein the preservation number is CGMCC No. 9977. The JZB42C003 strain is good in degrading effect on pendimethalin or beta-cypermethrin left in soil, fruits, vegetables or water bodies, and the degradation rate on 50mg / L pendimethalin in a basic inorganic salt culture solution treated by the JZB42C003 strain in 5 days can reach 68.5%, and the degradation rate on 50mg / L beta-cypermethrin in the basic inorganic salt culture solution treated by the JZB42C003 strain in 5 days can reach 55.7%.

The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.

The invention provides provindencia rettegeri Bg-7, and the preservation number of provindencia rettegeri Bg-7 is CGMCC No.9077. The provindencia rettegeri Bg-7 is Gram-negative bacterium, the contact enzyme reaction is positive, the oxidase reaction is negative, provindencia rettegeri Bg-7 can form milk-white bacterium groups on a MA culture medium (Difco, article number: 212185), the bacterium group is in a round shape, and the middle of the bacterium group projects slightly. The reaction that providencia rettgeri Bg-7 utilizes citrate to react is positive, and esculin iron citrate, 2-ketopotassium gluconate, mannitol, inositol, and glucose are fermented to produce acids. When the provided provindencia rettegeri Bg-7 is co-cultured with paralembus digitiformis of sea fishes, provindencia rettegeri Bg-7 can kill at least 97% of paralembus digitiformis in the culture system, and a novel method is provided for controlling sea fish diseases caused by paralembus digitiformis.

The invention relates to a strain of Providencia rettgeri L1 capable of efficiently degrading petroleum and an application thereof. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 26, 2018, an address is Institute of Microbiology, Chinese Academic Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and a strain preservationnumber is CGMCC No.16526. According to the invention, the strain of Providenia has a petroleum hydrocarbon degradation function, has differential selectivity on degradation of four components in petroleum, especially has a very remarkable degradation effect on n-alkanes of one of the petroleum components, can be applied to treatment of petroleum pollutants, and has a wide application prospect.

The invention relates to the technical field of biology, in particular to a nano-selenium-organic selenium nutrient solution and a preparation method and application thereof. The preparation method of the nano-selenium-organic selenium nutrient solution comprises the following steps that Providenciaretgeri is inoculated into a culture solution containing inorganic selenium for being fermented for 16 to 150 hours, so that the nano-selenium-organic selenium nutrient solution is obtained; and the preservation number of the providenciaretgeri is CCTCC M 2020956. The providenciaretgeri is facultative anaerobic bacteria, during large-scale fermentation culture, the requirement of strict aerobic bacteria on dissolved oxygen of the culture solution is avoided, and the requirement on fermentation equipment is relatively low; and meanwhile, the fermentation control difficulty is further reduced, and the fermentation process is easier to amplify.

The invention discloses a microbial agent for saline-alkali soil improvement. The microbial agent is composed of the components in parts by mass: 10%-35% of microbial bacteria, 20%-60% of an adsorbentand 20%-40% of phosphogypsum; the microbial bacteria comprise the components in percentage by mass: 15%-25% of pseudomonas, 20%-30% of bacillus subtilis, 10%-25% of bacillus megatherium, 15%-25% of providencia rettgeri, and 5%-15% of saccharomycetes; the microbial bacteria and ardealite are combined for use, dissolution and release of insoluble phosphate and solidified potassium can be accelerated, the content of available phosphorus, potassium and nitrogen in soil is increased for plants to absorb, and meanwhile the salinity and alkalinity of the soil are reduced.

The invention discloses a method for degrading antibiotics in livestock farm wastewater by the aid of ultraviolet irradiation processes. The method includes regulating a pH (potential of hydrogen) value of the livestock farm wastewater by acid and alkali solution; adding Providencia rettgeri solution and bacillus subtilis solution and preliminarily degrading the antibiotics; adding persulfate andferrous sulfate, forming advanced oxidation systems and then starting ultraviolet irradiation; carrying out oxidation / light degradation and continuing to degrade the antibiotics in biogas slurry; adding Tris-HCl solution and recycling the advanced oxidation systems; carrying out secondary oxidation / light degradation and degrading the antibiotics. The method has the advantages that the antibioticsin the wastewater can be degraded by the aid of technologies, and the method is high in degradation speed and degradation efficiency; good degradation effects can be realized, the antibiotics can be degraded into small-molecular substances, materials used in degradation procedures are low in cost and can come from extensive sources, and the antibiotics in the wastewater can be effectively degraded.

The invention discloses application of chelerythrine in inhibition and clearing of a multi-drug-resistant Providencia rettgeri biofilm. According to the application, the technical problem that the drug resistance of a drug-resistant Providencia rettgeri biofilm is difficult to clear once formed is solved. The chelerythrine is capable of inhibiting formation of the Providencia rettgeri biofilm, andthe minimum inhibition concentration is 15.6 micrograms per milliliter. Besides, the chelerythrine is capable of clearing a mature Providencia rettgeri biofilm, and the minimum clearing concentrationis 250 micrograms per milliliter. By virtue of the effect of a rooted whole grass extract, namely the chelerythrine of a papaveraceous plant celandine, on the multi-drug-resistant Providencia rettgeri biofilm, the infection caused by the Providencia rettgeri biofilm can be effectively relieved or solved, the healing rate is increased, new thoughts are provided for the inhibition of the Providencia rettgeri biofilm and the clearing of the mature biofilm, and the application has important practical significances.

The invention discloses a strain Pr-nj01 having insecticidal activity, an insecticidal agent and application thereof. The strain Pr-nj01 is identified as Providencia rettgeri, is preserved in the China center for type culture collection on May 10, 2017 and has a preservation number of CCTCC NO: M2017251. The separated and screened strain Pr-nj01 having insecticidal activity can be used for preventing and treating pests in fields, has broad-spectrum killing effects on pests in fields, can kill lepidoptera and hemiptera pests in fields and has good killing effects. The insecticidal agent is prepared through simple processes and has the advantages of low production cost, use convenience and good insecticidal effects. Through use of the insecticidal agent, chemical pesticide is reduced. The strain Pr-nj01 has the important significance for protecting the ecological environment and ensuring food safety.

The invention provides a degrading bacterium capable of eliminating plant fungicide residues and an application of the degrading bacterium. The degrading bacterium is identified to be providencia rettgeri, belongs to gram-negative bacteria, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 15, 2017; the preservation number is CGMCC NO. 14163. The degrading bacterium can grow with plant fungicide, bismerthiazol, as the only carbon source and energy, and the degrading rate for bismerthiazol with concentration of 100 mg / L in 96 h reaches 90% or higher; after direct application of the degrading bacterium, residues of field bismerthiazol can be significantly reduced, pollution can be effectively eliminated, the problem of over limits of the bismerthiazol residues in agricultural production is solved, and non-toxic and pollution-free green agricultural products are produced.

The invention discloses application of luteolin in inhibition of growth of multi-drug-resistant providencia rettgeri. According to the invention, because the luteolin has a good in-vitro killing effect on multi-drug-resistant providencia rettgeri, the growth of the multi-drug-resistant providencia rettgeri can be inhibited; and the minimum bactericidal concentration is 30 to 250 microgram / mL and the minimum bacteriostatic concentration is 15 to 125 microgram / mL. The luteolin has an inhibiting effect on the multi-drug-resistant providencia rettgeri and thus has the great application value in the fields of medicines and the like.

The invention discloses Providencia rettgeri with aniline degradation and denitrification capabilities and an application of the Providencia rettgeri. The strain is named as Providencia rettgeri Y15-7, is preserved in the CCTCC on August 26, 2019, and has a preservation number of CCTCC NO: M 2019664. The Providencia rettgeri Y15-7 can be used for relatively rapidly degrading aniline under anoxic and aerobic conditions, and a part of TN can be converted into gaseous nitrogen; and the aniline can be degraded and denitrified in the presence of a second carbon source such as glucose or sucrose andthe like, a yeast extract, peptone and other organic nitrogen under a high salinity condition, and the Providencia rettgeri has broad application prospects in actual wastewater treatment with complexwater quality.

The invention discloses a method for degrading antibiotics in biogas slurry by the aid of ultraviolet irradiation processes. The method includes adding Providencia rettgeri solution into the biogas slurry and preliminarily degrading the antibiotics; adding persulfate and ferrous sulfate, forming advanced oxidation systems and then starting ultraviolet irradiation; carrying out oxidation / light degradation and continuing to degrade the antibiotics in the biogas slurry; adding dithiothreitol solution and recycling the advanced oxidation systems; carrying out secondary oxidation / light degradationand degrading the antibiotics. The method has the advantages that the antibiotics in the biogas slurry can be degraded by the aid of technologies, and the method is high in degradation speed and degradation efficiency; good degradation effects can be realized, the antibiotics can be degraded into small-molecular substances, materials used in degradation procedures are low in cost and can come fromextensive sources, and the antibiotics in the biogas slurry can be effectively degraded.

The invention discloses an application of sanguinarine in inhibition and removal of a multi-drug-resistant providencia rettgeri biofilm and aims at solving the technical problems of drug resistance ofthe drug-resistant providencia rettgeri biofilm and difficulty in removal once the drug-resistant providencia rettgeri biofilm is formed. According to the fact that sanguinarine has a good providencia rettgeri biofilm inhibiting and removing effect, formation of the providencia rettgeri biofilm can be inhibited, and the lowest inhibition concentration of the biofilm is 7.81 micrograms per milliliter. The sanguinarine can be used for effectively removing a mature providencia rettgeri biofilm, and has the lowest biofilm removal concentration of 125 micrograms per milliliter. According to the invention, a natural product sanguinarine is adopted to inhibit and remove a multi-drug-resistant providencia rettgeri biofilm, can effectively relieve or solve the infection treatment difficulty causedby the providencia rettgeri biofilm, improves the cure rate, provides a new lead compound for inhibiting the formation of the providencia rettgeri biofilm and removing the mature biofilm, and has important practical significance.

The present invention relates to a strain of Providencia rettgeri (Providencia rettgeri) L1 that efficiently degrades petroleum and its application. The strain was preserved in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures on September 26, 2018, address : Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, strain collection number CGMCC No.16526. The present invention discovers for the first time that the bacterial strain of Providencia (Providencia) has the function of degrading petroleum hydrocarbons, and has differential selectivity to the degradation of four components in petroleum, especially for normal alkanes, one of the petroleum components. It has a very significant degradation effect, can be applied to the treatment of petroleum pollutants, and has broad application prospects.

The invention relates to a nucleic acid reagent and a digital PCR (polymerase chain reaction) kit for detecting providencia. The nucleic acid reagent comprises primers as shown in SEQ ID NO. 1-6 and probes as shown in SEQ ID NO. 9-11. According to the invention, a detection system for simultaneously detecting the providencia stuartii, the providencia alcaligenes and the providencia reevesii is established; a detection system contains an exogenous internal control as a contrast, and quality control can be carried out on the detection process; the detection result has good sensitivity and specificity.

The invention discloses application of citral in inhibition of growth of multi-drug resistant providencia rettgeri. The citral has better in-vitro killing effects on human-derived providencia rettgeriresisting ampicillin, ampicillin / sulbactam, cefazolin, cefotetan, ceftriaxone, ceftazidime, tobramycin, tazobactam and piperacillin, ciprofloxacin, levofloxacin, amikacin, cotrimoxazole, cefepime, ertapenem, imipenem, aztreonam and gentamicin, can inhibit growth of resistant providencia rettgeri, and has the minimum bactericidal concentration of 0.5 mg / mL and the minimum inhibitory concentrationof 0.25 mg / mL; and the inhibition function of the plant extract citral on the multi-drug resistant providencia rettgeri can relieve or solve the problem of increase of drug resistance of the multi-drug resistant providencia rettgeri and provide a theoretical basis for clinical antibacterial treatment and research and development of novel drugs.

The invention discloses the application of sanguinarine in the inhibition and removal of multi-drug-resistant Providence recei biofilms, and is used to solve the drug resistance of drug-resistant Providence recei biofilms and the formation of biofilms that are difficult to remove once formed. technical problem. According to the fact that sanguinarine has good biofilm inhibitory and scavenging effects on Providencia revei, it can inhibit the formation of biofilm of Providencia revei, and the minimum inhibitory concentration of biofilm is 7.81 μg / mL. Sanguinarine can effectively remove mature Providencia reinhardtii biofilm, and the minimum biofilm removal concentration is 125μg / mL. It uses the natural product sanguinarine to inhibit and clear the biofilm of the multi-drug-resistant Providencia raptorii, which can effectively alleviate or solve the difficulty in the treatment of infections caused by the biofilm of Providencia reinhardtii, and improve the cure rate. Inhibiting the formation of Providencia reinhardtii biofilms and clearing mature biofilms provides new lead compounds with important practical significance.

The invention discloses the application of luteolin in inhibiting the growth of multi-drug-resistant Providencia rettgeri. According to the fact that luteolin has a good in vitro killing effect on multi-drug-resistant Providencia rettgeri, it can inhibit multiple The growth of drug-resistant Providencia rettgeri, and the minimum bactericidal concentration is 30 ~ 250 μg / mL, the minimum inhibitory concentration is 15 ~ 125 μg / mL, the present invention proposes that luteolin is effective against multidrug-resistant Providencia rettgeri The inhibitory effect of S. spp. has wide application value in medicine and other fields.

The invention discloses a root-knot nematode biocontrol bacterium, a biocontrol bacterium agent and applications thereof. The biocontrol bacterium is Providencia rettgeri (Providencia rettgeri) strain UN06, and the preservation number is CGMCC No.10246. The present invention utilizes the fermented liquid obtained by the fermentation of the Providencia rettgeri UN06 strain to mix evenly with adsorption carriers such as bacterial residue and peat and urea, a nutrient substance required for bacterial growth, and then granulate to prepare the UN06 granule. The control effects of applying 100 kg of UN06 granules per mu on tomato root-knot nematode, cucumber root-knot nematode and tobacco root-knot nematode were 90.17%, 88.66% and 85.98%, respectively. The root-knot nematode biological control bacterial agent of the invention has the characteristics of low cost, high control effect, no residue, and safety to humans and animals, and is a high-efficiency biological pesticide for preventing and treating root-knot nematode.

The invention discloses a bacterial strain Pr-nj01 with insecticidal activity and its production and application of insecticides. The bacterial strain Pr-nj01 has been identified as Providencia rettgeri (Providencia rettgeri) and has been preserved in the Chinese Typical Culture Collection Center, the preservation time is May 10, 2017, and the preservation number is CCTCC NO: M2017251. The present invention obtains the bacterial strain Pr-nj01 with insecticidal activity through separation and screening, which can be used for the control of field pests. The bacterial strain has a broad-spectrum killing effect on field pests, and can kill field pests such as Lepidoptera and Hemiptera, and Good killing effect. The insecticide fungicide of the present invention is simple to prepare, has the advantages of low production cost, convenient use and good insecticidal effect. The use of the fungicide can reduce the use of chemical pesticides, which is of great significance for protecting the ecological environment and ensuring food safety.

The invention relates to a Providencia sp. strain MRP36 and application thereof; the strain is preserved in the Guangdong Microbial Culture Collection Center on May 11, 2021; and the preservation number is GDMCCNO: 61650. The strain has the characteristics of acid resistance, cadmium resistance, phosphorus solubilization, siderophore production and auxin secretion. The biomass, the chlorophyll SPAD value and the phosphorus content of soybean can be improved by inoculating the soybean with the Providencia sp. MRP36; furthermore, the inhibition effect of cadmium on soybean growth can be overcome; especially, the inhibition effect of cadmium on soybean root growth can be overcome; and the application value is very high.

The invention provides a degrading bacterium for eliminating plant fungicide residues and application thereof, the degrading bacterium is identified as Providencia rettgeri Providencia retgeri , belonging to Gram-negative bacteria, which was deposited in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC) on May 15, 2017, and the preservation number is: CGMCC NO.14163. As the only carbon source and energy source for growth, the degradation rate of 100 mg / L eubacazole within 96 hours can reach more than 90%. The direct application of degrading bacteria can significantly reduce the field phylloxazole residue, which can effectively eliminate pollution and solve the problem of It solves the problem of excessive phyllazole residues in agricultural production, and produces green agricultural products that are non-toxic and pollution-free.

The invention relates to a microbe composite bacterial liquid comprising live bacteria of clostridium thermopalmarium, clostridium thermosuccinogenes, brevibacillus borstelensis, paenibacillus barengoltzii, bacillus subtilis, pseudomonas mendocina, and providencia rettgeri. The invention also provides a bacterial preparation prepared by using the composite bacterial liquid. The bacterial preparation is used for preparing an organic fertilizer. According to the invention, with the composite microbe bacterial liquid, during a composite fertilizer utilization process, the dosage of inorganic composite fertilizer can be reduced, such that production cost can be reduced. Also, with a synergetic effect provided by the microbe bacterial preparation, when dosages of nitrogen, phosphorous and potassium in organic-inorganic composite fertilizers are low, agricultural and forestry waste stalks with low prices and rich organic substances such as nitrogen, phosphorous, potassium and calcium are converted into micro-molecular substances such as monosaccharide and oligosaccharide which can be utilized by plants. Therefore, a relatively high crop yield is obtained, and a substantial yield increasing effect is provided. Agriculture in our nation has a severe dependence on chemical fertilizers. With the composite bacterial liquid provided by the invention, the situation can be changed, and agricultural ecological protection can be enhanced.

The invention discloses a Providencia rettgeri strain and application thereof. The Providencia rettgeri YL is collected in China General Microbiological Culture Collection Center (CGMCC) on April 28th, 2013, and the collection number is CGMCC No.7544. The Providencia rettgeri is used for treating nitrogenous organic wastewater, and can effectively remove organic nitrogen, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen while removing organic carbon, and thus, the whole organic carbon and nitrogen synchronous removing process can be implemented only by one aerobic stage. The strain has the heterotrophic nitrification-aerobic denitrification function, can synchronously remove the organic carbon and nitrogen under aerobic conditions, can greatly reduce the occupied area and construction investment, can shorten the system start-up time, reduces the treatment cost, and enhances the treatment efficiency.