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Providencia rettgeri (commonly P. rettgeri), is a Gram negative bacterium that is commonly found in both water and land environments. P. rettgeri is in the genus Providencia, along with Providencia stuartii, Providencia alcalifaciens, and Providencia rustigianii. P. rettgeri can be incubated at 37 °C in nutrient agar or nutrient broth. It was first discovered in 1904 after a waterfowl epidemic. Strains of the species have also been isolated from nematodes of the genus Heterorhabditis.
Primers for triple pcr detection of morganella morganii, proteus vulgaris and providencia rettgeri and detection method thereof
ActiveCN118109617BMicrobiological testing/measurementMicroorganism based processesNucleotideAlligator
This invention discloses primers and a detection method for triple PCR detection of *Morganella morganii*, *Proteus vulgaris*, and *Providencera reticularis*. The primers are Mm-F, Mm-R, ureC-F, ureC-R, trpD-F, and trpD-R, with nucleotide sequences shown in SEQ ID NO. 1–6. This invention provides a triple PCR detection method for simultaneously detecting *Morganella morganii*, *Proteus vulgaris*, and *Providencera reticularis*. This method is highly specific, sensitive, rapid, and accurate, and can be used for clinical and laboratory PCR detection of *Morganella morganii*, *Proteus vulgaris*, and *Providencera reticularis* in alligator gout. It has broad application potential in rapid pathogen detection and epidemiological investigation of alligator gout.
Owner:NANJING AGRICULTURAL UNIVERSITY