4 results about "Providencia rettgeri" patented technology

The invention discloses bactrocera cucurbitae intestinal bacterium compound bacteria and application thereof, and relates to the technical field of biology. The bacterial compound bacteria comprise the providencia rettgeri M15 and the providencia mantanoxydans M52, the preservation number of the providencia rettgeri M15 is CGMCC (China General Microbiological Culture Collection Center) 1.62474, and the preservation number of the providencia mantanoxydans M52 is CGMCC 1.62475. The invention further discloses a preparation method of the bacterial compound bacteria, and the bacterial compound bacteria comprise the providencia rettgeri M15 and the providencia mantanoxydans M52. The compound bacteria are mainly used for improving the survival rate of bactrocera cucurbitae larvae.

The invention provides a biological flocculant, a preparation method thereof and application of the biological flocculant in treatment of fracturing flow-back fluid. The biological flocculating agent disclosed by the invention comprises a fermentation product of Providencia retinii sp. Hong 1 Xiao Zong, and can also selectively comprise a fermentation product of bacillus sp. JS Xiao Bai, and the biological flocculating agent disclosed by the invention can be used for preparing the biological flocculating agent. The biological flocculant disclosed by the invention has relatively good salt-resistant and alkali-resistant properties, and is suitable for flocculation treatment of the fracturing flow-back fluid.

The invention discloses a composite microbial inoculant for soil improvement, which is prepared from the following raw materials: Providencia retinii S17-1, Serratia sp. VL03-3, Pantoea eucallypti P2FPb1 (Pantoea eucallypti P2FPb1) and Bacillus proteolyticus 1372 (Bacillus proteolyticus 1372), and the composite microbial inoculant is prepared from the following raw materials: Pantoea eucallypti P2FPb1, Pantoea eucallypti P2FPb1, Pantoea eucallypti P2FPb1, Pantoea eucallypti P2FPb2, Pantoea eucallypti, Pantoea eucallypti, Pantoea eucallypti On the other hand, the invention discloses a preparation method of the compound microbial inoculant. The preparation method comprises the following steps: acquiring a single colony, preparing a single strain solution, mixing the single strain solution, and inoculating and culturing. The composite microbial inoculant has the composite growth promoting capacities of dissolving phosphorus, dissolving potassium, secreting plant hormones and the like, does not cause plant diseases, has good ecological adaptability, is safe to use and high in economic benefit, and does not threaten the ecological environment. The compound microbial inoculant disclosed by the invention can be used for increasing the plant yield, improving the soil enzyme activity and improving the soil quality and the rhizosphere micro-ecological environment.

This invention discloses primers and a detection method for triple PCR detection of *Morganella morganii*, *Proteus vulgaris*, and *Providencera reticularis*. The primers are Mm-F, Mm-R, ureC-F, ureC-R, trpD-F, and trpD-R, with nucleotide sequences shown in SEQ ID NO. 1–6. This invention provides a triple PCR detection method for simultaneously detecting *Morganella morganii*, *Proteus vulgaris*, and *Providencera reticularis*. This method is highly specific, sensitive, rapid, and accurate, and can be used for clinical and laboratory PCR detection of *Morganella morganii*, *Proteus vulgaris*, and *Providencera reticularis* in alligator gout. It has broad application potential in rapid pathogen detection and epidemiological investigation of alligator gout.