A strain of Providencia rettgeri capable of degrading aniline and denitrification and its application

A technology of Providencia rettgeri and aniline, applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms, can solve the problems of low salinity, no denitrification performance, and limited wastewater treatment, etc., and achieve broad The effect of applying the foreground

Active Publication Date: 2021-10-26
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the bacterium adapts to low salinity, and the NaCl concentration in the culture medium is only 4g / L, which will greatly limit its treatment of wastewater with high salinity, and there is no aerobic reaction of aniline organic wastewater using this bacterium. There are no research reports on the denitrification performance of the bacteria under anoxic conditions.
[0005] At present, in the research on the degradation of aniline by pure culture strains, no strains have been reported that have the ability to degrade aniline and denitrify under both anoxic and aerobic conditions.

Method used

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  • A strain of Providencia rettgeri capable of degrading aniline and denitrification and its application
  • A strain of Providencia rettgeri capable of degrading aniline and denitrification and its application
  • A strain of Providencia rettgeri capable of degrading aniline and denitrification and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Screening, isolation and identification of bacterial strains capable of degrading aniline and denitrification:

[0025] Source of the strain: activated sludge of printing and dyeing wastewater

[0026] LB medium (g / L): bacteriological peptone 10, yeast extract 5, NaCl 10, distilled water, pH 7.0.

[0027] Screening and isolation method of bacterial strains: Take 10 mL of pretreated sludge, inoculate it into 90 mL of LB medium containing 200 mg / L aniline, and culture it in a shaker at 30°C and 160 r / min for 5 days . Then transfer to concentrations of 300 mg / L, 400 mg / L, 500 mg / L, 600 mg / L, 800 mg / L for acclimatization. Then, the sludge acclimatization culture solution was serially diluted in 10-fold increments, spread on the LB solid medium plate containing 200 mg / L aniline, and cultured in a biochemical incubator at 30°C to obtain a single colony. A single colony was picked and inoculated in the aniline degradation medium, and the aniline removal rate and T...

Embodiment 2

[0033] Example 2 Degradation and denitrification characteristics of strain Y15-7 to aniline under aerobic conditions

[0034] Seed medium for culturing strain Y15-7 (g / L): glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4•7H2O 0.5, KH2PO4 0.5, K2HPO4 1.5, KNO3 1.0, aniline 0.2, Distilled water, pH7.0.

[0035]The medium used to study the degradation ability of bacteria Y15-7 (g / L): glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4•7H2O 0.5, KH2PO4 0.5, K2HPO4 1.5, KNO3 1.0, aniline 0.4 , distilled water, pH7.0.

[0036] Inoculate the Y15-7 strain preserved on the plate into the seed medium, and cultivate it at 30°C and 160 r / min for 42 h to obtain the seed liquid; ℃, 160 r / min, aerobic aeration and shaking culture for 72 hours, samples were taken at different time points to determine the contents of aniline, total nitrogen, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, intracellular nitrogen and OD in the solution 600 , to...

Embodiment 3

[0037] Example 3 Degradation and denitrification efficiency of aniline by strain Y15-7 under different conditions of oxygen supply

[0038] Seed medium for culturing strain Y15-7 (g / L): glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4•7H2O 0.5, KH2PO4 0.5, K2HPO4 1.5, KNO3 1.0, aniline 0.2, Distilled water, pH7.0.

[0039] The medium used to study the degradation ability of bacteria Y15-7 (g / L): glucose 2, bacteriological peptone 1, yeast extract 0.5, NaCl 10, MgSO4•7H2O 0.5, KH2PO4 0.5, K2HPO4 1.5, KNO3 1.0, aniline 0.4 , distilled water, pH7.0.

[0040] Inoculate the Y15-7 strain preserved on the plate into the seed medium, and cultivate it at 30°C and 160 r / min for 48 hours to obtain the seed liquid; inoculate the seed liquid with 1% inoculum amount until it contains 400 mg / L aniline for degradation In the culture medium, different oxygen supply environments (strong aerobic, aerobic, anoxic) were created by changing the amount of the reactor solutio...

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Abstract

The invention discloses a strain of Providencia rettgeri with the ability to degrade aniline and denitrify and its application. The bacterium is named Providencia rettgeri (Providencia rettgeri) Y15‑7 and was released on August 26, 2019. It was deposited in the China Center for Type Culture Collection, and the deposit number is CCTCC NO: M 2019664. The Providencia rettgeri Y15-7 can degrade aniline quickly under anoxic and aerobic conditions, and can convert part of TN into gaseous nitrogen, and can be used in second carbon sources such as glucose or sucrose and In the presence of organic nitrogen such as yeast extract and peptone, and under high salinity conditions, the degradation and denitrification of aniline has broad application prospects in the actual wastewater treatment with complex water quality.

Description

technical field [0001] The invention relates to a strain of Providencia rettgeri capable of degrading aniline and denitrification and its application, belonging to the technical field of environmental microorganisms and wastewater treatment. Background technique [0002] Aniline is an important chemical raw material for the production of dyes, plastics, pesticides and pharmaceuticals, and its annual demand is huge. Mass production and wide application make it a pollutant in many chemical industry effluents. Due to the "three causes" characteristics of high toxicity, refractory degradation, carcinogenicity, teratogenicity and mutagenicity, aniline has been listed as "environmental priority pollutants" by the United States, China and other countries. Microbial degradation is considered to be the most effective method for aniline removal in aqueous environments. Aniline will release ammonia nitrogen during the biodegradation process. Ammonia nitrogen is an important substance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/20C12N1/205C12R2001/01
Inventor 张金丽陈子和李志朋张琼
Owner JIMEI UNIV
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