Cadmium-resistant growth-promoting Providencia sp. strain and application thereof

A technology of Providencia rettgeri and strains, applied in the field of microorganisms, can solve the problems of cadmium intolerance, achieve the effects of promoting phosphorus content, improving cadmium resistance, and overcoming growth inhibition

Active Publication Date: 2021-10-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this bacterial strain is intolerant to cadmium, can not dissolve insoluble phosphorus and allow plants to absorb and utilize phosphorus, and cannot solve the technical problems of cadmium toxicity stress and low phosphorus stress of plants

Method used

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  • Cadmium-resistant growth-promoting Providencia sp. strain and application thereof
  • Cadmium-resistant growth-promoting Providencia sp. strain and application thereof
  • Cadmium-resistant growth-promoting Providencia sp. strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Separation and purification of bacterial strains

[0047] 1. Isolation and purification of strains

[0048] In Beiyue Village, Qujiang District, Shaoguan City, strains were isolated from the rhizosphere of maize (Zhengtian 68) grown on cadmium-contaminated soil. Cut 5g of corn roots respectively, brush off the surface soil with a small brush, rinse with sterile water several times until there is no attached soil, put it in a 250mL numbered triangular bottle filled with 100mL of sterilized water, 180r / min, 37℃ Shaker, shake 30min. Add 10-20 glass beads to the Erlenmeyer flask to help break up the soil, so that the bacteria can be released from the soil. After the shaking is over, let the soil suspension stand for 10 minutes to obtain a soil suspension. Take the supernatant and carry out 10 times Serial gradient concentration dilution, diluted to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 , 10 -6 , then pipette 0.1mL of the diluent and spread it on the LB pla...

Embodiment 2

[0050] Embodiment 2: strain screening

[0051] 2.1 Primary screening of phosphate-dissolving bacteria

[0052] The screened bacterial strains were inoculated on PKO solid medium, and each plate was spotted three times, cultured in a 28°C incubator, and the outer diameter (D) and colony diameter (d) of the strain were measured on the 7th day and photographed. Those with a phosphorus-dissolving circle can dissolve phosphorus. Then compare the ratio of (D / d) to judge the phosphorus-dissolving ability of the strain.

[0053] Take 1mL of the bacterial solution of the strain with a phosphorus-dissolving circle, inoculate it into 20mL LB liquid medium (50mL centrifuge tube), and measure the OD after 24 hours of cultivation 600 1.0, take 1mL of bacterial suspension and inoculate in 20mL PKO liquid medium (50mL centrifuge tube), repeat three times for each strain, CK control group is inoculated with the same amount of sterile water, placed at 28°C, 180r / min in a shaker. Measure th...

Embodiment 3

[0069] Example 3 strain identification

[0070] After comprehensive consideration in many aspects, the screened strain MRP36 was identified.

[0071] 3.1 Morphological identification of strains

[0072] The strain MRP36 was inoculated on LB solid medium for cultivation, and observation records were made. After culturing for 5-7 days under the optimum growth conditions (pH 7.0, temperature 37°C), the isolated and purified strain MRP36 was observed for single colony status, mainly including colony size, color, colony surface status and colony edge status, etc. . On the other hand, for the bacterial strain MRP36 in the logarithmic growth phase, the morphology of the bacterial cells was observed with an optical microscope after the smear was stained.

[0073] On the LB plate medium, approximately round, smooth, protruding, milky white colonies are formed, Gram staining is red, and Gram-negative bacteria.

[0074] 3.2 Molecular identification

[0075] After various screening a...

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Abstract

The invention relates to a Providencia sp. strain MRP36 and application thereof; the strain is preserved in the Guangdong Microbial Culture Collection Center on May 11, 2021; and the preservation number is GDMCCNO: 61650. The strain has the characteristics of acid resistance, cadmium resistance, phosphorus solubilization, siderophore production and auxin secretion. The biomass, the chlorophyll SPAD value and the phosphorus content of soybean can be improved by inoculating the soybean with the Providencia sp. MRP36; furthermore, the inhibition effect of cadmium on soybean growth can be overcome; especially, the inhibition effect of cadmium on soybean root growth can be overcome; and the application value is very high.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to Providencia rettgeri strain MRP36 and its application. Background technique [0002] Cadmium is not an essential element for plant growth and development. When the content in the body exceeds a certain concentration, the plant will be seriously harmed, such as wilting and yellowing of the plant, weak growth and other symptoms. Plant roots can easily absorb cadmium, and then transport it to the aboveground, which will poison the plants, affect the absorption of nutrient elements, and then inhibit the growth of plants. Cadmium can also damage root structure, thereby affecting plant nutrient uptake. [0003] Phosphorus is one of the most important essential nutrients for plants and is an essential macronutrient for key metabolic processes such as plant cell division, energy generation, macromolecular biosynthesis, membrane integrity, signal transduction, and photosynthesis....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00A01G7/06A01G22/40A01C21/00C12R1/01
CPCA01G22/40A01G7/06A01C21/00A01N63/20
Inventor 王秀荣王幼娟罗莎莉陆星
Owner SOUTH CHINA AGRI UNIV
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