Degrading bacterium capable of eliminating plant fungicide residues and application of degrading bacterium

A plant fungicide, degrading bacteria technology, applied in the direction of application, microbe-based methods, bacteria, etc., can solve the problem of decreased control effect, achieve the effect of increasing added value, good removal effect, and solving the problem of excessive pesticide residues

Active Publication Date: 2017-09-19
中持(江苏)环境建设有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yebuazole is a kind of systemic fungicide for the prevention and control of bacterial diseases independently developed by our country. It has special effects on rice bacterial blight and bacterial streak disease, and can also be widely used in the control of fruit tree ulcers; thiazolein Fungicides such as furamide, tebuconazole, azoxystrobin, copper hydroxide, and chlorobromoisocyanuric acid have significant effects on various fungal diseases of peppers, rice, vegetables, fruit trees, corn, and wheat. Bacterial and fungal diseases occur, and due to the long-term use of pesticides, the resistance of crop pathogens has increased, and the control effect has begun to decline

Method used

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  • Degrading bacterium capable of eliminating plant fungicide residues and application of degrading bacterium
  • Degrading bacterium capable of eliminating plant fungicide residues and application of degrading bacterium
  • Degrading bacterium capable of eliminating plant fungicide residues and application of degrading bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Screening and isolation of ectacazole degrading Providencia rettgeri CS-7

[0020] Get 3.0 ml of activated sludge obtained from the waste water treatment tank of a certain pesticide factory production workshop that produces eclozazole and add it into 100 ml of inorganic salt liquid culture that has a concentration of 100 mg / L of ebacazole. The formula is: 1.50 g K 2 HPO 4 , 0.50 gKH 2 PO 4 , 0.20 g MgSO 4 ·7H 2 O, 1.00 g NaCl, 1.00 g (NH 4 ) 2 SO 4 , pH 7.0, shake culture at 160 r / min, 30°C, transfer to fresh inorganic salt medium every 5 days at 3% inoculum size, and transfer 5 times in a row.

[0021] Take 1.0 ml of the enriched bacterial solution obtained above, add it into 9.0 ml sterile water, and make 10 -1 enriched solution, and then draw 1.0 ml prepared 10 -1 The enrichment solution was added to 9.0 ml sterile water, mixed thoroughly to make 10 -2 The enrichment solution, and so on, carry out gradient dilution on the enrichment solution. Dr...

Embodiment 2

[0023] Embodiment 2 Shake flask degradation experiment of Providencia rettgeri CS-7

[0024] In the inorganic salt medium containing 100 mg / L eubazole, the formula is: 1.50 g K per liter 2 HPO 4 , 0.50gKH 2 PO 4 , 0.20 g MgSO 4 ·7H 2 O, 1.00 g NaCl, 1.00 g (NH 4 ) 2 SO 4 , pH 7.0, inoculate CS-7 at 3% inoculum size, culture at 30°C with shaking, and take samples every 12 h for determination. Depend on figure 2 It can be seen that the absorption peak of the treated sample decreased significantly, and eubazole was degraded by more than 90%.

Embodiment 3

[0025] Embodiment 3 Contains the preparation of Providencia rettgeri CS-7 bacterial agent

[0026] Activate the original species of eubazole-degrading Providencia rettgeri CS-7 on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. The test tube seed was inoculated in a 1000 ml shake flask containing 200 ml LB medium. The formula of LB medium was: yeast extract 5.00 g / L, peptone 10.00 g / L, NaCl 10.00 g / L, pH 7.0, constant temperature shaking culture until In several phases, prepare the seed pots for inoculation. The seed tank is 500 L, the feeding volume is 400 L, and the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.0. After the feeding is completed, sterilize under high pressure at 121°C, cool to 33°C, inoculate 10% of the cultured shake flask strains into a 500 L seed tank, and cultivate to the logarithmic gr...

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Abstract

The invention provides a degrading bacterium capable of eliminating plant fungicide residues and an application of the degrading bacterium. The degrading bacterium is identified to be providencia rettgeri, belongs to gram-negative bacteria, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 15, 2017; the preservation number is CGMCC NO. 14163. The degrading bacterium can grow with plant fungicide, bismerthiazol, as the only carbon source and energy, and the degrading rate for bismerthiazol with concentration of 100 mg / L in 96 h reaches 90% or higher; after direct application of the degrading bacterium, residues of field bismerthiazol can be significantly reduced, pollution can be effectively eliminated, the problem of over limits of the bismerthiazol residues in agricultural production is solved, and non-toxic and pollution-free green agricultural products are produced.

Description

technical field [0001] The invention belongs to the technical field of biodegradation, and in particular relates to a degrading bacterium for eliminating plant fungicide residues and an application thereof. Background technique [0002] my country is a big agricultural country. In recent years, the bacterial and fungal diseases of crops in our country have become more and more serious. When the diseases are serious, they can cause a large reduction in production or even no harvest, which brings a heavy burden to farmers. Yebuazole is a kind of systemic fungicide for the prevention and control of bacterial diseases independently developed by our country. It has special effects on rice bacterial blight and bacterial streak disease, and can also be widely used in the control of fruit tree ulcers; thiazolein Fungicides such as furamide, tebuconazole, azoxystrobin, copper hydroxide, and chlorobromoisocyanuric acid have significant effects on various fungal diseases of peppers, ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/14C09K101/00C12R1/01
CPCC09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/01
Inventor 陈立伟林举媚董晓梦余静
Owner 中持(江苏)环境建设有限公司
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