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Providencia rettgeri Bg-7

A technology of Providencia reais, Gram-negative bacteria, applied in the field of microbial screening

Active Publication Date: 2015-08-12
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of microorganisms to antagonize and prevent pathogenic scutellaria in seawater fish.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] The screening culture of embodiment 1 Bg-7 bacterial strain

[0011] Step 1. Bacterial Strain Separation and Purification: The original sample collection site of the obtained bacterial strain Bg-7 of the present invention is the Indian Ocean (E91 ° 1'10 "; N10 ° 0'10 "), after the sample collection, immediately aseptically apply to MA On the 2216 medium (Difco, product number: 212185), use the coated petri dish upside down at 25°C to incubate for 24-96 hours, pick out the single colony that grows, and streak on the MA 2216 medium plate for purification. Place the marked petri dish upside down at 25°C and incubate for 24-96 hours, pick out the single colony that grows, and then streak on the MA 2216 medium plate for purification, and place the marked petri dish upside down at 25°C Cultivate for 24-96 hours. After a single colony grows, pick a single colony and put it into 1ml seawater 2216E liquid medium, and seal it with a parafilm for later use. The seawater 2216E liqu...

Embodiment 2

[0017] Example 2 Identification and Preservation of Bacterial Strain Bg-7

[0018] Step 1. Physiological and biochemical analysis of bacterial strain: the physiological and biochemical identification of bacterial strain Bg-7 is operated with reference to " common bacterial system identification handbook ", and identification result is that this bacterium is Gram-negative bacteria, and its contact enzyme reaction is positive, and oxidase reaction is Negative, milky white colonies are formed on MA medium (Difco, product number: 212185), the colonies are round, with slight protrusions in the middle, the bacteria react positively with citrate, ferment aesculin ferric citrate, 2- Potassium ketogluconate, potassium 2-ketogluconate, mannitol, inositol, glucose produce acid.

[0019] Step 2. Amplification and sequence analysis of the 16S rRNA gene of the strain

[0020] Preparation of 16S rRNA gene template: Pick a single colony from the Bg-7 storage spare plate and put it on the MA ...

Embodiment 3

[0023] The insecticidal effect of embodiment 3 Bg-7 bacterial strain culture fluid

[0024] Step 1. Strain cultivation: Pick a single colony of Bg-7 from a preserved plate and put it in 1 ml of seawater 2216E liquid medium, place it at 25°C and shake it for 36-96 hours until it reaches its 0D 600 When it reaches 0.4-0.7, transfer it as seed liquid to 100 ml of seawater 2216E liquid medium, place it at 25°C and shake it for 36-96 hours until its 0D 600 Reach 0.4-0.7 and seal with parafilm for later use.

[0025] Step 2. Preparation of shield ciliate liquid: take the shield ciliate liquid preserved in the laboratory, and after 10-fold gradient dilution, pick the drop containing 1 ciliate and immediately transfer it to 1ml fish broth medium and place it at 14-18°C for cultivation. Wait until the density of shield ciliates reaches 5×10 in the fish soup medium 3When individual / ml is above, transfer this 1ml fish soup culture medium to 50ml fish soup culture medium and place 14-18...

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PUM

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Abstract

The invention provides provindencia rettegeri Bg-7, and the preservation number of provindencia rettegeri Bg-7 is CGMCC No.9077. The provindencia rettegeri Bg-7 is Gram-negative bacterium, the contact enzyme reaction is positive, the oxidase reaction is negative, provindencia rettegeri Bg-7 can form milk-white bacterium groups on a MA culture medium (Difco, article number: 212185), the bacterium group is in a round shape, and the middle of the bacterium group projects slightly. The reaction that providencia rettgeri Bg-7 utilizes citrate to react is positive, and esculin iron citrate, 2-ketopotassium gluconate, mannitol, inositol, and glucose are fermented to produce acids. When the provided provindencia rettegeri Bg-7 is co-cultured with paralembus digitiformis of sea fishes, provindencia rettegeri Bg-7 can kill at least 97% of paralembus digitiformis in the culture system, and a novel method is provided for controlling sea fish diseases caused by paralembus digitiformis.

Description

technical field [0001] The invention belongs to the technical field of microbial screening, and relates to Providencia rettgeri. Background technique [0002] Pathogenic scutellum ciliates are one of the most harmful diseases in the industrial culture of marine fish in my country, and their occurrence has become more frequent in recent years. Scutellum ciliates are facultative parasites, generally free-living, and feed on suspended tiny particulate matter (bacteria, microalgae, protozoa, etc.); however, under certain circumstances, these ciliates can be parasitic, Phagocytosis of fish cells and tissue residues and growth and reproduction in their tissues; reports show that shield ciliates can occur in factory farming workshops across my country from 12 to 26 °C, almost all year round; shield ciliates The disease is the most severe in the flounder, and the mortality rate of more than 90% will occur if the control is not effective. At present, the scutellaria ciliates found in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P33/02C12R1/01
Inventor 曲凌云田欣欣王琛尚琨
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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