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A strain of Providencia sp. 2D that efficiently degrades dibutyl phthalate

A technology of Providence bacteria and strains, which is applied in the field of biological treatment of environmental pollutants, can solve problems such as incomplete biodegradation, and achieve the effects of simple culture method, less variation and strong adaptability.

Active Publication Date: 2018-05-01
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the ubiquity of DBP in the environment, the frequency of DBP-degrading bacteria is also very high. At present, most of the microorganisms that degrade DBP are Gordonella, Agrobacterium, Acinetobacter and Rhodococcus. The reported biodegradation of DBP by these bacteria is not complete, and they will produce more toxic intermediate metabolites, or their degradation rate cannot meet the requirements of actual pollution control, so it is still necessary to screen more efficient obligate or facultative degrading bacteria

Method used

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  • A strain of Providencia sp. 2D that efficiently degrades dibutyl phthalate
  • A strain of Providencia sp. 2D that efficiently degrades dibutyl phthalate
  • A strain of Providencia sp. 2D that efficiently degrades dibutyl phthalate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and identification of dibutyl phthalate degrading bacteria

[0020] 1. Medium formula

[0021] Inorganic salt culture medium (MSM, g / L): K 2 HPO 4 : 5.8; KH 2 PO 4 : 4.5; (NH 4 ) 2 SO 4 : 2.0; MgCl 2 :0.16; CaCl 2 :0.02; Na 2 MoO 4 ·2H 2 O: 0.0024; FeCl 3 : 0.0018; MnCl 2 ·2H 2 O: 0.0015; adjust the final pH of the inorganic salt culture solution to 7.5, add DBP to make the concentration in the inorganic salt culture solution 50 mg / L, and DBP as the sole carbon source.

[0022] Beef extract peptone medium (LB): 5.0 g yeast powder, 10.0 g peptone, 10.0 g sodium chloride, add ultrapure water to 1L, adjust pH=7.0. Sterilize at 121°C for 20 minutes. For solid plates, add 1.5% (W / V) agar powder.

[0023] 2. Separation and identification

[0024] The separation method adopts the sample suspension shake flask method: weigh 5 g of compost sample (taken from farm animal compost) in a 150 mL Erlenmeyer flask containing 50 mL of sterile water, culture at 30°C and 140 rp...

Embodiment 2

[0029] Example 2 Providencia sp. 2D degradation experiment of DBP and its intermediate product phthalic acid

[0030] 1. Preparation of bacterial suspension

[0031] The purified strain Providencia sp. 2D was connected to 10 mL of LB liquid medium for overnight activation and culture to the logarithmic phase. The bacteria were collected by centrifugation at 5000 rpm for 10 min, washed with PBS for 3 times and resuspended, and adjusted the OD 600 nm = 0.8 as a bacterial suspension.

[0032] 2. Determination of degradability

[0033] Inoculate 1 mL of MSM culture medium containing different concentrations of DBP (50, 100, 200, 500, 1000 mg / L) and phthalic acid (25, 50, 100, 200, 500 mg / L) respectively into 100 mL, With no inoculation as a control, and adjust the pH to 8.0, three replicates in each group. Incubate at 30°C, 140 rpm constant temperature shaker for 6 days, take samples regularly, and determine the degradation of DBP and phthalic acid by GC / MS.

[0034] Chromatographic co...

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Abstract

The invention belongs to the technical field of biological treatment of environmental pollutants, and specifically discloses a high-efficiency Providencia sp. 2D that can completely degrade dibutyl phthalate. The strain was released on January 22, 2015 It was deposited in the China Center for Type Culture Collection (CCTCC) in Japan, and the deposit number is CCTCC M 2015057. The strain degraded more than 80% of the high-concentration DBP (1000 mg / L) in 72 h, and almost completely degraded the low-concentration DBP (200 mg / L); the strain was also able to degrade phthalic acid, an intermediate product of DBP , and eventually DBP is completely mineralized. The strain has high-efficiency DBP degradation ability, which greatly supplements the database of DBP-degrading bacteria. It has a good application prospect.

Description

Technical field [0001] The present invention relates to the technical field of biological treatment of environmental pollutants, and more specifically, to a Providence strain that is highly efficient and can completely degrade dibutyl phthalate ( Providencia sp.) 2D. Background technique [0002] Dibutyl phthalate (di- n -butylphthalate (DBP) is a phthalic acid ester compound (Phthalic acidester, PAEs), one of the most important plasticizers in the plastics industry, as well as a carrier for pesticides, dye detergents, and raw materials for the production of many cosmetics and textiles. It is widely used in food packaging materials, containers, medical equipment and children's toys. Due to the large number of applications of the above products, DBP has become a global organic pollutant, which is widely present in the atmosphere, water, soil and organisms. Especially in agricultural production, due to the wide application of plastic films and plastic greenhouses, DBP remaining i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/205C12R2001/01
Inventor 莫测辉赵海明蔡全英李彦文李慧冯乃宪杜欢喻娇
Owner JINAN UNIVERSITY
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