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A strain of Providencia and its application in the preparation of antibacterial drugs

A technology of Providencia bacteria and antibacterial drugs, applied in the direction of antibacterial drugs, methods based on microorganisms, bacteria, etc., to achieve the effects of preventing hazards, ensuring human health and safety, and improving water quality

Active Publication Date: 2016-05-11
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a Providencia (providenciavermicola) No. 22, which can effectively eliminate the Vibrio alginolyticus in the aquaculture system with the effect of antagonistic bacteria, and solve the problem of algae-dissolving bacteria carried by aquatic products in the prior art. Anti-epidemic problems of Vibrio

Method used

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  • A strain of Providencia and its application in the preparation of antibacterial drugs
  • A strain of Providencia and its application in the preparation of antibacterial drugs
  • A strain of Providencia and its application in the preparation of antibacterial drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Screening and Identification of Providencia Vermicola No. 22

[0014] (1) Search and screening of antagonistic bacteria of Vibrio alginolyticus

[0015] (A) Raw materials

[0016] Vibrio alginolyticus

[0017] Antagonistic strains to be screened: 311 strains were isolated from Xisha waters, lobster intestines, sea cucumber intestines, etc.

[0018] Medium reagent used: LB medium, purchased from a reagent company.

[0019] LB medium formula: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L.

[0020] (B) Preparation of Vibrio alginolyticus solution: Vibrio alginolyticus was inoculated into LB medium with an inoculation loop through aseptic operation, and cultivated overnight at 30°C and 200rpm.

[0021] (C) Isolation and screening of antagonistic bacteria of Vibrio alginolyticus

[0022] (1) Bacteria isolation

[0023] Through aseptic operation, the lobster intestines sampled in Xisha were ground with PBS and diluted into a 10-fold gradient, 100u...

Embodiment 2

[0034] Embodiment 2: Preparation of antagonistic bacteria preparation (fermentation agent)

[0035] According to the biological characteristics of the best Vibrio alginolyticus antagonist Providencia No. 22 obtained through screening, a suitable culture medium was screened out, and the antagonistic bacteria was prepared into a fermentation broth using appropriate fermentation conditions.

[0036] Concretely adopted fermentation conditions are as follows:

[0037] Fermentation strain: Vibrio alginolyticus antagonist Providencia No. 22.

[0038] Fermentation medium formula: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1L.

[0039] Fermentation temperature: 30°C

[0040] Fermentation cycle: 48-72h

[0041] Rotational speed of fermentation shaker: 150-200rpm

[0042] According to the above fermentation conditions, ferment to obtain Vibrio alginolyticus antagonistic bacteria starter, the pH of which is 8.0-8.5. The concentration of Providencia No. 22 re...

Embodiment 3

[0043] Embodiment 3: Artemia application simulation experiment

[0044] Weigh 0.2g of Artemia (brine shrimp) eggs into 500ml of sterile seawater, and hatch overnight at 30°C under the condition of pumping air at a constant temperature. Use a 12-well plate to do experiments, prepare in advance the Vibrio alginolyticus antagonistic bacteria starter fermented in Example 2, put 2ml sterile seawater and 30 Artemia in each well, and add only Vibrio alginolyticus (10 8 cfu / ml), only add Vibrio alginolyticus antagonistic bacteria starter (10 8 cfu / ml), and Vibrio alginolyticus (10 8 cfu / ml) and Vibrio alginolyticus antagonistic bacteria starter (10 8 cfu / ml), the control group did not add any bacteria, only Artemia. Three parallels were set up in each group, and the conditions were not changed during the experiment. After 4 days of observation, the mortality rate of each group was counted. The specific results are shown in Table 2 and figure 2 shown.

[0045] Table 2: Survival ...

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Abstract

The invention discloses providencia vermicola and application of providencia vermicola in preparing antibacterial drugs. The preservation number of providencia vermicola No. 22 is CCTCC No. M2013696. The providencia vermicola No. 22 has antagonism, so that the providencia vermicola No. 22 can be used for preparing preparation for preventing and controlling vibrio alginolyticus or preparing assistant hemolysis vibrio preparation; the preparation for preventing and controlling vibrio alginolyticus or the assistant hemolysis vibrio preparation can be used for beneficial microbial preparation to replace chemical medicine, improves water quality, and eliminates vibrio alginolyticus or assistant hemolysis vibrio in an aquatic product cultivation system, so that damage to aquatic product cultivation objects from the vibrio alginolyticus or assistant hemolysis vibrio is prevented, the development of the aquatic product cultivation industry is promoted, aquatic products are prevented from carrying the vibrio alginolyticus or assistant hemolysis vibrio, and health and safety of human beings are ensured.

Description

Technical field: [0001] The invention belongs to the field of aquaculture, and in particular relates to a Providencia strain with high stability of anti-active components, which can be used as an antagonistic bacteria of various bacteria and its application in the preparation of antibacterial drugs. Background technique: [0002] Vibrio alginolyticus (V.alginolyticus) belongs to Vibrio family (Vibrionaceae), Vibrio (Vibrio), the bacteria exist in seawater, estuaries, and marine animals. Vibrio alginolyticus has strong pathogenicity to fish, shrimp, shellfish and other farmed animals, and can cause acute hemorrhagic sepsis in farmed animals. This disease, also known as explosive epidemic, vibriosis, body surface ulcer, etc., can infect a variety of mariculture species, such as large yellow croaker, sea bass, flounder, spotted grouper, black sea bream, flat sea bream, cobia , Penaeus chinensis, Penaeus japonica, Penaeus monodon, Litopenaeus vannamei, clams, squid, etc. are con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/741A61P31/04C12R1/01
Inventor 陈偿韦露胡超群
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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