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Nano-selenium-organic selenium nutrient solution and preparation method and application thereof

A technology of organic selenium and nano-selenium, applied in the biological field, can solve the problems of limited scale application, high production cost, high equipment and process control requirements, and achieve the effects of avoiding dissolved oxygen demand, high production efficiency, and easy fermentation process amplification

Active Publication Date: 2021-05-04
武汉华硒生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because it is a photosynthetic bacterium, the cultivation conditions require special lighting equipment compared with ordinary bacteria, and the requirements for equipment and process control are higher, and the high production cost limits its large-scale application.

Method used

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  • Nano-selenium-organic selenium nutrient solution and preparation method and application thereof
  • Nano-selenium-organic selenium nutrient solution and preparation method and application thereof

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preparation example Construction

[0036] Among the present invention, the preparation method of described Providencia rettgeri seed liquid seed liquid comprises the following steps:

[0037] (1) Strain activation: inoculate Providencia rettgeri on LB solid medium or beef extract peptone solid medium, and place it in a 37°C incubator for constant temperature cultivation for 16-48h to obtain activated strains;

[0038] (2) Preparation of seed solution: inoculate the activated bacteria into LB liquid medium or beef extract-peptone liquid medium, and cultivate for 16-24 hours to obtain the seed solution.

[0039] In a preferred embodiment, the OD of the seed solution 600 The value is 0.5-2.5.

[0040] In a preferred embodiment, the volume ratio of the seed solution to the culture solution containing inorganic selenium is (0.01-1):1.

[0041] culture medium containing inorganic selenium

[0042] In the present invention, the chemical valence of selenium in the inorganic selenium is +4 or +6.

[0043] In a pre...

Embodiment 1

[0065] Embodiment 1 provides a kind of preparation method of nano selenium-organic selenium nutrient solution, comprises the following steps:

[0066] (1) Activation of strains: Inoculate Providencia rettgeri (Providenciarettgeri) on beef extract peptone solid medium, and place it in a 37°C incubator for constant temperature cultivation for 24 hours to obtain activated strains;

[0067] (2) Seed solution preparation: inoculate the activated strain in the beef extract peptone liquid medium, cultivate until the OD value of the seed solution is 1.5, and obtain the seed solution;

[0068] (3) Inoculation: insert the seed liquid into the liquid medium containing inorganic selenium, the volume ratio of the seed liquid and the medium containing inorganic selenium is 0.05:1, ferment and cultivate for 120h, to obtain final product;

[0069] The components of the medium containing inorganic selenium are: beef extract 15g / L, peptone 8g / L, sodium chloride 6g / L, sodium selenite 445mmol / L, ...

Embodiment 2

[0071] Embodiment 2 provides a kind of preparation method of nano selenium-organic selenium nutrient solution, comprises the following steps:

[0072] (1) Activation of strains: Inoculate Providencia rettgeri (Providenciarettgeri) on beef extract peptone solid medium, and place it in a 37°C incubator for constant temperature cultivation for 24 hours to obtain activated strains;

[0073](2) Seed solution preparation: inoculate the activated strain in the beef extract peptone liquid medium, cultivate until the OD value of the seed solution is 1.5, and obtain the seed solution;

[0074] (3) Inoculation: insert the seed liquid into the liquid medium containing inorganic selenium, the volume ratio of the seed liquid and the medium containing inorganic selenium is 0.05:1, ferment and cultivate for 120h, to obtain final product;

[0075] The components of the medium containing inorganic selenium are: beef extract 15g / L, peptone 8g / L, sodium chloride 6g / L, sodium selenite 250mmol / L, a...

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Abstract

The invention relates to the technical field of biology, in particular to a nano-selenium-organic selenium nutrient solution and a preparation method and application thereof. The preparation method of the nano-selenium-organic selenium nutrient solution comprises the following steps that Providenciaretgeri is inoculated into a culture solution containing inorganic selenium for being fermented for 16 to 150 hours, so that the nano-selenium-organic selenium nutrient solution is obtained; and the preservation number of the providenciaretgeri is CCTCC M 2020956. The providenciaretgeri is facultative anaerobic bacteria, during large-scale fermentation culture, the requirement of strict aerobic bacteria on dissolved oxygen of the culture solution is avoided, and the requirement on fermentation equipment is relatively low; and meanwhile, the fermentation control difficulty is further reduced, and the fermentation process is easier to amplify.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nano-selenium-organic selenium nutrient solution and a preparation method and application thereof. Background technique [0002] Selenium is an essential trace element that is very important to humans and animals. It plays an important role in anti-cancer, anti-oxidation and enhancing immunity, and many chronic diseases are related to selenium deficiency in the human body. However, my country is a big selenium-deficient country. According to relevant geological surveys, as many as 72% of my country's areas are selenium-deficient and low-selenium areas. From Heilongjiang Province in the northeast to the Qinghai-Tibet Plateau in the southwest, there is a selenium-deficient belt distribution area. Most of the provinces and cities along the way are selenium-deficient or low selenium strip. Residents living in selenium-deficient or low-selenium areas have a series of problems due to in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C05F11/08C05F17/20C05G5/20C05G1/00C12R1/01
CPCC12P3/00C05D9/02C05F11/08C05F17/20C05G5/20Y02W30/40
Inventor 刘涛刘宏辉
Owner 武汉华硒生物科技有限公司
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