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A Root Knot Nematode Biocontrol Bacteria, Biocontrol Bacteria and Its Application

A technology for root-knot nematodes and biocontrol fungicides, applied in the directions of nematicides, applications, bacteria, etc., to achieve the effect of high control efficiency and low cost

Active Publication Date: 2018-07-24
广西泰宝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nematicidal properties of this microorganism have not been reported

Method used

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Embodiment 1

[0026] Embodiment 1: the isolation of Providencia rettgeri (Providencia rettgeri) bacterial strain UN06 bacterial strain

[0027] The present inventor isolated and purified a batch of endophytic bacteria from the stems of tomato in Shouguang greenhouse in Shandong Province using a selective medium, and screened out Providence retwig with high efficiency for killing root-knot nematodes after bioassays on root-knot nematodes. Providencia rettgeri strain UN06. The strain was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on December 26, 2014 (Address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing), and the preservation number: CGMCC No.10246 .

[0028]The biological characteristics of the bacterial strain are as follows: the colonies on the AMS medium plate are milky white, 2 mm in diameter, translucent, round and smooth. The bacteria are rod-shaped, w...

Embodiment 2

[0030] Embodiment 2: the preparation of Providencia rettgeri (Providencia rettgeri) bacterial strain UN06 biocontrol agent

[0031] Beef extract peptone solid medium, the formula is: beef extract 5g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, pH 7.2-7.4;

[0032] Beef extract peptone liquid medium, the formula is: beef extract 5g, peptone 10g, NaCl 5g, water 1000mL, pH7.2-7.4;

[0033] SRC medium, the formula is: sucrose 12.5g, rice flour 50g, crab shell powder 37.5g, NaCl 2.5g, MgSO 4 1.25g, KH 2 PO 4 2.5g, add water to make up to 1000ml, pH 6.5.

[0034] The preparation method specifically comprises the following steps:

[0035] (1) Cultivation of slant seeds: the thallus of the bacterial strain was moved to the slant of beef extract peptone solid medium, and cultivated at 36° C. for 2 days to obtain slant seeds;

[0036] (2) Liquid seed culture: inoculate the slant seeds into the beef extract-peptone liquid medium in the triangular flask, and culture on a shaker at ...

Embodiment 3

[0039] Embodiment 3: Field test of UN06 granule to control greenhouse tomato root-knot nematode

[0040] Test site: vegetable greenhouse in Shouguang City, Shandong Province.

[0041] Experimental crop: tomato (variety: Fendu-80).

[0042] Test agent: a. The number of live bacteria is 3.6×10 9 Each / gram of UN06 granules; b. 2% Abamectin EC.

[0043] Test method: The following three treatments were set up, each treatment had 3 replicates, and each replicate had 120 tomato plants.

[0044] Treatment 1: 100 kg / mu UN06 granules. When preparing the site, spread UN06 granules evenly on the soil surface at an amount of 75 kg / mu, and mix it into the soil with a tiller; apply 10 grams of UN06 granules to the roots when transplanting (according to For 2500 plants, the application rate in holes is 25 kg / mu).

[0045] Treatment 2: Dilute 2% Abamectin EC with water 1000 times for root irrigation.

[0046] Treatment 3: blank control without any nematicide application.

[0047] Invest...

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Abstract

The invention discloses a root-knot nematode biocontrol bacterium, a biocontrol bacterium agent and applications thereof. The biocontrol bacterium is Providencia rettgeri (Providencia rettgeri) strain UN06, and the preservation number is CGMCC No.10246. The present invention utilizes the fermented liquid obtained by the fermentation of the Providencia rettgeri UN06 strain to mix evenly with adsorption carriers such as bacterial residue and peat and urea, a nutrient substance required for bacterial growth, and then granulate to prepare the UN06 granule. The control effects of applying 100 kg of UN06 granules per mu on tomato root-knot nematode, cucumber root-knot nematode and tobacco root-knot nematode were 90.17%, 88.66% and 85.98%, respectively. The root-knot nematode biological control bacterial agent of the invention has the characteristics of low cost, high control effect, no residue, and safety to humans and animals, and is a high-efficiency biological pesticide for preventing and treating root-knot nematode.

Description

technical field [0001] The invention relates to a root-knot nematode biocontrol bacterium, a biocontrol bacterium and an application thereof in preventing and treating root-knot nematode disease, belonging to the technical field of microbial pesticides. Background technique [0002] Diseases caused by pathogenic nematodes of crops have become the second largest category of diseases in agricultural production. The direct economic loss caused by crop pathogenic nematodes is as high as 157 billion U.S. dollars (Abad P, Gouzy J, Aury JM.etal.2008.Genome sequence of the metazoan plant-parasitic nematode Meloidogyneincognita.Nat.Biotechnol.26(8):909 -15.doi:10.1038 / nbt.1482.), among which the root-knot nematode is the most harmful and causes the greatest loss. Root-knot nematode can harm more than 3,000 kinds of plants such as grain, oilseeds, vegetables, tobacco, and fruits. Root-knot nematodes infect crop roots with second-instar larvae and induce nematode root nodules in plant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N25/12A01P5/00C12R1/01
CPCA01N25/12A01N63/00C12N1/20C12N1/205C12R2001/01A01N2300/00
Inventor 周峰莫明和潘克静
Owner 广西泰宝生物科技有限公司
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