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Strain for degrading pendimethalin pesticide and fungicide and application of strain

A technology of pendimethalin and bacterial strains, which is applied to the pendimethalin pesticide degrading strains and its bacterial agents and application fields, which can solve the problems of complex mechanism, environmental production, and low degradation efficiency, etc., and achieve good degradation effect

Active Publication Date: 2015-04-22
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Pendimethalin is very stable in the soil, has a long half-life in the environment, and is difficult to degrade quickly under natural conditions. In addition, frequent use for a long time may cause phytotoxicity of subsequent crops and pose a potential risk to the environment
[0005] However, the number of microbial strains that can efficiently degrade pendimethalin is still relatively limited, and the degradation efficiency is not very high, and the mechanism of using microorganisms to degrade pesticides is very complicated and highly targeted

Method used

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  • Strain for degrading pendimethalin pesticide and fungicide and application of strain
  • Strain for degrading pendimethalin pesticide and fungicide and application of strain
  • Strain for degrading pendimethalin pesticide and fungicide and application of strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Isolation and purification of Providencia rettgeri (Providencia rettgeri) JZB42C003

[0043] The potato farmland soil samples from Daxing District, Beijing, where pendimethalin was applied were divided into 2 parts, each 10 g, and were added to 100 mL enrichment medium containing pendimethalin under aseptic conditions (wherein , the concentration of pendimethalin in the enrichment medium is 50mg / L), cultivated on a shaker at 120r / min at 30°C for 7d; "Refer to the volume ratio of the inoculum and the inoculated medium) and transfer it to the next batch of enrichment medium containing pendimethalin (wherein, in this batch of enrichment medium, the concentration of pendimethalin is 100 mg / L), continue to cultivate for 7 days; then transfer to the enrichment medium containing pendimethalin (the concentration of pendimethalin in the enrichment medium is 200mg / L) according to the inoculum size of 10% , and then cultivated for 7d; then transferred to the basic i...

Embodiment 2

[0044] Embodiment 2: 16SrDNA identification of Providencia rettgeri (Providencia rettgeri) JZB42C003

[0045] 1. Acquisition of PCR template: it includes two methods, one is to extract genomic DNA to use it as a PCR template, and the other is to directly heat-treat the bacterial liquid to use the heat-treated bacterial liquid as a PCR template. Both methods are described below.

[0046] 1) Genomic DNA was extracted, using lysozyme plus 10% SDS method, as follows:

[0047] (a) Inoculate the purified bacterium in LB liquid medium, culture on a shaker at 180r / min at 28°C for 24h, then take 2ml of fresh culture solution, centrifuge at 12000rpm for 1-2min, and discard the supernatant ;

[0048] (b) Add 1mL, 1×TE (pH8.0) buffer to the precipitate, mix and wash, centrifuge at 12000rpm for 1-2min, and discard the supernatant;

[0049] (c) Add 400 μL, 5×TE (pH8.0) buffer to the precipitate, mix and wash, centrifuge at 12000 rpm for 1-2 min, and discard the supernatant;

[0050] (d)...

Embodiment 3

[0065] Example 3: Determination of the degradation effect of pendimethalin by strain JZB42C003 at different times

[0066] 1) Cultivate the purified bacterial strain JZB42C003 in LB liquid medium to the logarithmic period (the logarithmic period is determined by measuring the OD600 value, and the OD600 value reaches 0.8, which is the logarithmic period), and then inoculum with 10% (this implementation In the example, "10% inoculum size" refers to the volume ratio of the inoculum to the inoculated medium) is inserted into the basic inorganic salt culture solution containing pendimethalin as 50mg / L, at 30°C, at a constant temperature of 120r / min Vibrating culture on a shaker for 10 days, the 1, 3, 5, 7 and 10-day culture solution was used for the determination of the residual amount of pendimethalin, and at the same time, the basic inorganic salt culture solution without bacteria was set as a control, and each treatment was 3 repeat. After the cultivation, absorb 2 mL of the su...

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Abstract

The invention discloses a strain for degrading a pendimethalin pesticide and a fungicide and an application of the strain. The strain is Providencia rettgeri JZB42C003 which is preserved at CGMCC on 17th, November, 2014, wherein the preservation number is CGMCC No. 9977. The JZB42C003 strain is good in degrading effect on pendimethalin or beta-cypermethrin left in soil, fruits, vegetables or water bodies, and the degradation rate on 50mg / L pendimethalin in a basic inorganic salt culture solution treated by the JZB42C003 strain in 5 days can reach 68.5%, and the degradation rate on 50mg / L beta-cypermethrin in the basic inorganic salt culture solution treated by the JZB42C003 strain in 5 days can reach 55.7%.

Description

technical field [0001] The invention relates to a bacterial strain for degrading pesticides, in particular to a bacterial strain for degrading pendimethalin pesticides, bacterial agents and applications thereof. Background technique [0002] According to reports, my country's pesticide soil pollution is as high as 140 million mu. The problem of excessive pesticide residues in vegetables and fruits has become a trade barrier restricting the export of agricultural products in my country. Acute and chronic poisoning incidents also occur from time to time. The widespread and high-dose use of organic phosphorus, pyrethroids, heterocyclics and other pesticides not only seriously affects the food safety and quality of agricultural products, but also causes pollution of soil, surface water and groundwater. [0003] Pendimethalin belongs to dinitroaniline herbicides, chemical name: N-(ethylpropyl)-3,4-dimethyl-2,6-dinitroaniline. Pendimethalin is mainly used to prevent and control a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/04A62D101/28A62D101/26
CPCA62D3/02A62D2101/04A62D2101/26A62D2101/28C12N1/20C12N1/205C12R2001/01
Inventor 陈莉李文华贾春虹余苹中朱晓丹贺敏赵尔成
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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