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Providencia rettgeri strain and application thereof

A common technology of Providencia revii, applied to a strain of Providencia revii and its application field, can solve problems such as lack of application, and achieve the effects of fast growth rate, stable performance and good removal effect

Inactive Publication Date: 2015-07-15
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, the research on this new type of heterotrophic bacteria is still in its infancy, and its application in water treatment processes is relatively lacking.

Method used

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  • Providencia rettgeri strain and application thereof
  • Providencia rettgeri strain and application thereof
  • Providencia rettgeri strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0044] Embodiment 1: Providencia rettii ( Providencia retgeri ) YL for ammonia nitrogen organic wastewater treatment

[0045] Place Providencia rettgeri YL in ammonia nitrogen (NH 4 + -N) in the simulated wastewater as the only nitrogen source, the investigation of strain YL on NH 4 + -N and organic carbon (TOC) removal efficiencies. Artificial water distribution is (per liter): NH 4 Cl 0.688 g, glucose 4.5 g, NaCl 4 g, NaCl 2 HPO 4 2.66 g, KH 2 PO 4 1 g, trace element solution 3 mL. Each liter of trace element solution contains MgSO 4 ·7H 2 O 3 g, MnSO 4 3 g, ZnSO 4 ·7H 2 O 3 g, H 3 BO 3 1.12 g, FeSO 4 ·7H 2 O 0.3 g, CaCl 2 0.6 g. The culture conditions were 30°C and 120 rpm. The experimental results are attached figure 2 shown. Strain YL grew well in the simulated wastewater, and reached the maximum growth in 24 hours, and NH 4 + -N was removed rapidly, and the removal rate reached 18.7 mg N / L / h. After 96 hours of cultivation, the removal ...

Embodiment approach 2

[0046] Embodiment 2: Providencia rettii ( Providencia retgeri ) YL for nitrous nitrogen organic wastewater treatment

[0047] Place Providencia rettgeri YL in the presence of nitrite (NO 2 - -N) was the only nitrogen source in the simulated wastewater, and the strain YL was investigated for NO 2 - -N and TN removal efficiency. Artificial water distribution is (per liter): NaNO 2 0.049 g, glucose 0.249 g, NaCl 4 g, Na 2 HPO 4 2.66 g, KH 2 PO 4 1 g, trace element solution 3 mL. Each liter of trace element solution contains MgSO 4 ·7H 2 O 3 g, MnSO 4 3 g, ZnSO 4 ·7H 2 O 3 g, H 3 BO 3 1.12 g, FeSO 4 ·7H 2 O 0.3 g, CaCl 2 0.6 g. The culture conditions were 30°C and 120 rpm. The experimental results are attached image 3 shown. Strain YL can rapidly remove NO in wastewater 2 - -N, after 48h, NO 2 - The removal rate of -N reaches over 98%, and the removal rate of TN is about 83%.

Embodiment approach 3

[0048] Embodiment 3: Providencia rettii ( Providencia retgeri ) YL for nitrate organic wastewater treatment

[0049] Providencia rettgeri YL was exposed to nitrate (NO 3 - -N) was the only nitrogen source in the simulated wastewater, and the strain YL was investigated for NO 3 - -N and TN removal efficiency. Artificial water distribution is (per liter): NaNO 3 0.061 g, glucose 0.25 g, NaCl 4 g, Na 2 HPO 4 2.66 g, KH 2 PO 4 1 g, trace element solution 3 mL. Each liter of trace element solution contains MgSO 4 ·7H 2 O 3 g, MnSO 4 3 g, ZnSO 4 ·7H 2 O 3 g, H 3 BO 3 1.12 g, FeSO 4 ·7H 2 O 0.3 g, CaCl 2 0.6 g. The culture conditions were 30°C and 120 rpm. The experimental results are attached Figure 4 shown. Strain YL vs NO 3 - The removal rate of -N is 93%, and the removal rate of TN is about 90%.

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Abstract

The invention discloses a Providencia rettgeri strain and application thereof. The Providencia rettgeri YL is collected in China General Microbiological Culture Collection Center (CGMCC) on April 28th, 2013, and the collection number is CGMCC No.7544. The Providencia rettgeri is used for treating nitrogenous organic wastewater, and can effectively remove organic nitrogen, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen while removing organic carbon, and thus, the whole organic carbon and nitrogen synchronous removing process can be implemented only by one aerobic stage. The strain has the heterotrophic nitrification-aerobic denitrification function, can synchronously remove the organic carbon and nitrogen under aerobic conditions, can greatly reduce the occupied area and construction investment, can shorten the system start-up time, reduces the treatment cost, and enhances the treatment efficiency.

Description

technical field [0001] The present invention relates to a strain of Providencia rettii ( Providencia retgeri ) and its application in wastewater treatment. The strain has the function of heterotrophic nitrification-aerobic denitrification, and can effectively remove organic nitrogen, ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and their mixture in water under completely aerobic conditions, and simultaneously remove organic carbon in wastewater , especially suitable for the biological treatment of high-concentration nitrogen-containing organic wastewater. Background technique [0002] Biological treatment of wastewater is a widely used method of wastewater treatment. Although the aerobic biological treatment process represented by activated sludge method and biofilm method has good removal effect on organic carbon in wastewater, but the removal effect on nitrogen is not good. Therefore, for nitrogen-containing organic wastewater, additional treatment structures ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01
CPCY02W10/15Y02W10/10
Inventor 安强赵彬叶君陈杰吕清浩
Owner CHONGQING UNIV
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