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Lysobacter antibioticus HY strain and application thereof

A technology of antibiotic lysobacteria and microbial strains, applied in the direction of application, bacteria, biocide, etc., can solve the problems of unsatisfactory control effects, etc., and achieve the effects of good growth promotion effect, easy industrial production, and strong antibacterial ability

Inactive Publication Date: 2013-12-04
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, agricultural cultivation measures, disease-resistant varieties, and chemical control measures are mainly used to prevent and control the above diseases, but the control effects are not satisfactory.
[0006] According to the literature search, there is no public report on the use of the antibiotic Lysobacterium HY to prepare biological agents and apply them to control cruciferous vegetable clubroot, tobacco black shank and konjac soft rot in field.

Method used

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  • Lysobacter antibioticus HY strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Pharmacodynamic test of biocontrol bacteria HY preparation on Chinese cabbage clubroot

[0024] (1) In vitro antibacterial test of biocontrol strains

[0025] 1. Test material :

[0026] 1.1 Test strains: Antibiotic Lysobacter ( Lysobacter antibioticus ) HY.

[0027] Test pathogens

[0028] Plant pathogenic bacteria (Konjac Bacterial Soft Rot, Anthurium Leaf Phytophthora, Rice Bacterial Blight, Rice Bacterial Stripe, Chinese Cabbage Soft Rot, Cabbage Black Rot, Potato Ralstonia, Tobacco Wildfire Bacteria) , Pathogenic fungi (Phytophthora notoginseng, Fusarium oxysporum, Rhizoctonia solani, Tobacco black shank) were provided by the Bacterial Laboratory of the National Engineering Center for Biodiversity and Disease Control, Yunnan Agricultural University.

[0029] For reagents and culture medium bacteria

[0030] NA medium (5g peptone, 3g beef extract, 1g yeast extract, 10g sucrose, 17g agar, 1000mL water, pH 7.0); PDA medium (200g potat...

Embodiment 2

[0038] Embodiment 2: Greenhouse disease control effect of biocontrol bacteria HY preparation on Chinese cabbage clubroot

[0039] 1. Experimental materials and methods

[0040] 1.1. Test bacteria : Bio-control bacteria HY liquid

[0041] 1.2. Test crops : Chinese cabbage

[0042] 1.3. Prevention and control objects : Chinese cabbage clubroot

[0043] 1.4. Experimental site : In the greenhouse of the College of Plant Protection, Yunnan Agricultural University, potted Chinese cabbage variety 83-1 with high susceptibility to clubroot disease was set up with 3 replicates for each treatment.

[0044] Preparation of pathogenic bacteria and biocontrol agents:

[0045] 1.5.1 Preparation of inoculum

[0046] Take 15 g of fresh homogenate for each germ, prepare 100 mL of bacterial liquid, and use the colony counting method to make the spore concentration of the bacterial liquid to be 12 × 10 7 Add the bacterial solution to 300 g of air-dried peat soil, stir evenly,...

Embodiment 3

[0066] Embodiment 3: Field efficacy test of biocontrol bacteria HY preparation for preventing and treating Chinese cabbage clubroot

[0067] Chinese cabbage seeds were sown, and the variety was Chengza No. 5. The test materials were randomly arranged, and each material was planted in 15 rows, with 6 seedlings in each row, and three replicates were set. The experimental site was in Guanwa, Lufeng County, Yunnan Province.

[0068] The solid preparation of biocontrol bacterial agent HY was diluted 100 times, 200 times and 500 times with water, respectively, and the bacteria suspension of the prepared biocontrol strain HY was irrigated 7 days, 14 days and 21 days after sowing by watering the roots. root. Each Chinese cabbage seedling was irrigated with 200 mL of bacteria.

[0069] The 2000-fold dilution of the control fungicide Cyfamid was used to water the roots in the same way as above.

[0070] Blank control: water was applied, roots were irrigated by the same method a...

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Abstract

The invention relates to a lysobacter antibioticus HY strain and application thereof and belongs to the technical field of plant protection. The lysobacter antibioticus HY strain has already been preserved in the China General Microbiological Culture Collection Center on July 11, 2013 and the preservation number is CGMCC No.7912. The konjak rhizosphere bacteria lysobacter antibioticus HY strain is used for preparation of a medicine used for preventing and treating brassicaceous vegetable clubroots, tobacco black shanks and konjak bacterial soft rots in fields. The lysobacter antibioticus HY strain is efficient, non-toxic, safe, free of residue, capable of achieving the disease-control purpose by means of live bacteria, good in plant growth promoting effect, good in comprehensive performance, and suitable for industrialized production, bulk production and application.

Description

technical field [0001] The invention relates to an antibiotic lysobacterium HY and its application, belonging to the technical field of plant protection. Background technique [0002] Cruciferous crops include rapeseed and bulk vegetables, such as Chinese cabbage, cabbage, cauliflower, kohlrabi, stem mustard, mustard greens, radish, Chinese cabbage, mustard mustard (mustard) and special crops such as Radix isatidis and wasabi, etc., where clubroot occurs every year Reaching more than 20 million mu (times), especially once it occurs, the disease will increase year by year. Clubroot of cruciferous plants is caused by Plasmodium brassicae ( Plasmodiophora brassicae Woron) infection. The occurrence of cruciferous clubroot seriously affects the yield of vegetables. In extreme cases, it leads to a 70%-80% reduction in yield, and even makes vegetable farmers lose their harvest. Cruciferous crops cannot be planted on the same plot. Due to the large-scale and long-distance introdu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/01
Inventor 姬广海刘峰魏兰芳何月秋周丽洪方敦煌
Owner YUNNAN AGRICULTURAL UNIVERSITY
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