Method and applications for fast detecting pathogenic bacteria molecules of bacterial soft rotting disease for banana

Abstract

The invention discloses a method and applications for fast detecting pathogenic bacteria molecules of bacterial soft rotting disease for banana. The method comprises the steps that: a specific primer is utilized to conduct PCR (polymerase chain reaction) on DNA contained in a sample, and the sample contains the pathogenic bacteria molecules of bacterial soft rotting disease for banana when the PCR product presents a 171bp condition. The method is simple to operate, takes less time and is large in flux. The method is used for conducting determined detection to banana seedlings, soil leach liquor of soil in a planting area, irrigation water and the like, thus realizing the purposes of construction and safety production in no disease banana seedling production base in China and simultaneously stopping of introduction of dangerous foreign disease banana.

Description

technical field

[0001] The invention belongs to the fields of crop disease prevention and plant quarantine, and in particular relates to a method and application for rapid detection of pathogenic bacteria of banana bacterial soft rot disease, that is, using polymerase chain reaction (PCR) molecular biology technology to detect banana bacterial soft rot The high-sensitivity and rapid detection of disease pathogens can be used for banana seedling quarantine, early diagnosis of banana soft rot in the field, and monitoring and identification of pathogens. Background technique

[0002] The pathogen of banana bacterial soft rot is Pectobacterium chrysanthemi, which belongs to the genus Erwinia of the family Enterobacteriaceae. The pathogenic bacteria is highly pathogenic and may be a highly pathogenic subspecies. This is a new dangerous disease that has invaded my country. It was first discovered in September 2009 in the banana planting area of ​​Panyu, Guangzhou. The disease is...

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