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Bacillus amyloliquefaciens strain and application thereof in prevention and control of celery bacterial soft rot

A technology of amylolytic spores and bacilli, which is applied in the fields of application, pest control, bacteria, etc., can solve the problems of difficult breeding for disease resistance, and achieve the effect of preventing and controlling celery soft rot

Active Publication Date: 2019-08-16
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Breeding for disease resistance is more difficult, and agricultural measures also have greater limitations

Method used

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  • Bacillus amyloliquefaciens strain and application thereof in prevention and control of celery bacterial soft rot
  • Bacillus amyloliquefaciens strain and application thereof in prevention and control of celery bacterial soft rot
  • Bacillus amyloliquefaciens strain and application thereof in prevention and control of celery bacterial soft rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1. Isolation, screening and identification of Bacillus amyloliquefaciens ZF75

[0067] Several strains were isolated from rhizosphere soil collected from greenhouses where celery has been continuously grown for many years in Wuqing District, Tianjin. A single colony was picked and cultured in LB liquid medium to obtain a bacterial suspension; the celery stem method was used to screen out the bacterial strains with antibacterial effect on celery soft rot from the selected colonies (see Table 1). Among them, the ZF75 strain was significantly better than other strains in controlling soft rot of celery.

[0068] Morphological characteristics of strain ZF75: ZF75 is a gram-positive bacteria, rod-shaped, endophytic spores, without flagella.

[0069] Strain ZF75 formed milky white colonies on beef extract peptone medium (LB medium) with wrinkles and irregular edges. figure 1 The colony morphology of strain ZF75.

[0070] The optimum growth temperature of the strain ZF75 is 28...

Embodiment 2

[0076] Example 2. Preparation of Bacillus amyloliquefaciens ZF75 bacterial suspension

[0077] A single colony of purified Bacillus amyloliquefaciens ZF75 was inoculated in LB liquid medium and cultured at 28°C at 180r / min for 2 days to produce large amounts of spores of Bacillus amyloliquefaciens. The obtained bacterial suspension contains the effective content of the Bacillus amyloliquefaciens of 10 7 ~10 9 cfu / mL.

Embodiment 3

[0078] Example 3 Simple identification of the inhibitory effect of Bacillus amyloliquefaciens ZF75 on soft rot fungus of celery

[0079] Pick a single colony of Bacillus amyloliquefaciens ZF75, inoculate it in LB liquid medium, and culture it with shaking at 28°C for 24 hours for use. Place 7 5 cm long celery segments in vertical stacks. 8 Cfu / mL Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZF75 bacterial suspension in the humidifier, it is appropriate to soak 0.5-1cm stems in the bacterial suspension. After soaking the bacteria for 20 minutes, take out the stems, place them on filter paper evenly horizontally, and let them air dry for 1 hour indoors. Then in the same way, the celery soft rot fungus (Pectobacterium carotovorum) bacterial suspension (concentration 1×10 8 cfu / mL) soak in 1h. Observe the infection of pathogenic bacteria every 8h. When the incidence of the water control treatment reaches more than 90%, investigate the disease index of each treatment celery...

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Abstract

The invention discloses a bacillus amyloliquefaciens strain and application thereof in prevention and control of celery bacterial soft rot. The bacillus amyloliquefaciens refers to bacillus amyloliquefaciens ZF75 whose preservation number is CGMCC No.16777 in the China General Microbiological Culture Collection Center. The bacillus amyloliquefaciens ZF75 is a celery soil rhizospheric microorganismwhich is safe to humans, animals and crops and environmental-friendly. The bacillus amyloliquefaciens strain has strong antagonism to the celery bacterial soft rot. The bacillus amyloliquefaciens strain is adopted for biocontrol of the celery bacterial soft rot, which is an effective and stable biocontrol manner for the celery bacterial soft rot, the characteristics that the bacillus amyloliquefaciens strain is prone to acting on celery plants and has strong antagonism to the celery bacterial soft rot are made use of, and the requirement on celery bacterial soft rot prevention and control inagricultural production can be satisfied better.

Description

Technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a strain of Bacillus amyloliquefaciens and its application in preventing and treating soft rot of celery. Background technique [0002] Celery soft rot is a worldwide epidemic disease caused by Pectobacterium carotovorum (Pectobacterium carotovorum). It is one of the important diseases in the production of celery, which seriously affects the production of celery. In the early stage, it appeared water-soaked transparent soft rot, and later turned yellow and gradually turned dark brown, accompanied by a fishy smell. In severe cases, the entire plant was weakened and rotted, causing huge economic losses to growers. [0003] The prevention and treatment of soft rot of celery has been attached great importance to at home and abroad for many years. At present, the use of chemical pesticides for prevention and treatment has problems such as environmental pressure ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/10A01N63/00A01N63/02A01P1/00A01P3/00A01P21/00C12R1/07
CPCC12N1/20C12P17/10A01N63/00C12R2001/07C12N1/205A01N63/10
Inventor 谢学文李宝聚郑斐李磊石延霞柴阿丽
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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