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A strain of Bacillus amyloliquefaciens and its application in the control of celery soft rot

A technology of amylolytic spores and bacilli, which is applied in the fields of application, pest control, bacteria, etc., can solve the problems of difficult breeding for disease resistance, and achieve the effect of preventing and controlling celery soft rot

Active Publication Date: 2020-12-08
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Breeding for disease resistance is more difficult, and agricultural measures also have greater limitations

Method used

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  • A strain of Bacillus amyloliquefaciens and its application in the control of celery soft rot
  • A strain of Bacillus amyloliquefaciens and its application in the control of celery soft rot
  • A strain of Bacillus amyloliquefaciens and its application in the control of celery soft rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1. Isolation, screening and identification of Bacillus amyloliquefaciens ZF75

[0067] Several strains were isolated from rhizosphere soil collected from greenhouses where celery had been continuously planted for many years in Wuqing District, Tianjin. Pick a single colony and culture it in LB liquid medium to obtain a bacterial suspension; use the celery stem section method to screen out the strains with antibacterial effect on celery soft rot from the above-mentioned selected colonies (see Table 1). Among them, the strain numbered ZF75 has significantly better control effect on celery soft rot than other strains.

[0068] Morphological characteristics of strain ZF75: ZF75 is a Gram-positive bacterium, rod-shaped, endophytic spores, and without flagella.

[0069] Strain ZF75 formed milky white colonies on beef extract peptone medium (LB medium), with folds and irregular edges. figure 1 Colony morphology of strain ZF75.

[0070] The optimal growth temperature...

Embodiment 2

[0076] Embodiment 2, the preparation of bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZF75 bacterial suspension

[0077] A single colony of purified Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZF75 was inoculated in LB liquid medium and cultured at 28° C. at 180 r / min for 2 days to produce a large number of spores of Bacillus amyloliquefaciens. Gained bacterium suspension contains the effective content of described bacillus amyloliquefaciens is 10 7 ~10 9 cfu / mL.

Embodiment 3

[0078] Embodiment 3, simple identification of the inhibitory effect of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZF75 on celery soft rot pathogen

[0079] Pick a single colony of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZF75, inoculate it in LB liquid medium, and culture it with shaking at 28°C for 24 hours for later use. 8 cfu / mL of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZF75 bacterial suspension in a humid box, it is advisable to soak 0.5-1cm stalks in the bacterial suspension. After soaking for 20 minutes, the stems were taken out, placed horizontally and evenly on the filter paper, and air-dried indoors for 1 hour. Then in the same way in the celery soft rot pathogen (carrot soft rot pectin bacillus (Pectobacterium carotovorum)) bacteria suspension (concentration 1 × 10 8 cfu / mL) for 1h. The infection of pathogenic bacteria was observed every 8 hours. When the incidence rate of the water control treatment reached more than 90...

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Abstract

The invention discloses a strain of bacillus amyloliquefaciens and its application in preventing and treating celery soft rot. The invention provides an understanding of Bacillus amyloliquefaciens ZF75, whose preservation number in the General Microorganism Center of China Microorganism Culture Collection Management Committee is CGMCC No.16777. The bacillus amyloliquefaciens ZF75 provided by the invention is a celery soil rhizosphere microorganism, which is safe for humans, livestock and crops, and friendly to the environment. The strain has a strong antagonistic effect on celery soft rot. Utilizing the strain for biocontrol of celery soft rot is a highly efficient and stable biocontrol method for celery soft rot, which utilizes the easy action of the Bacillus amyloliquefaciens on celery plants and strong antagonism to celery soft rot The characteristics of the function can better meet the requirements of the prevention and treatment of celery soft rot in agricultural production.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a strain of bacillus amyloliquefaciens and its application in preventing and treating soft rot of celery. Background technique [0002] Celery soft rot is a worldwide epidemic disease caused by Pectobacterium carotovorum. It is one of the important diseases in celery production and seriously affects the yield of celery. In the early stage, water-soaked transparent soft rot appeared, and in the later stage, it turned yellow and gradually turned dark brown, accompanied by a fishy smell. In severe cases, the entire plant was paralyzed and rotted, causing huge economic losses to the growers. [0003] For the prevention and treatment of celery soft rot, great attention has been paid to both at home and abroad for many years. At present, there are problems such as environmental pressure and pathogen resistance caused by the use of chemical pesticides for p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/10A01N63/22A01P1/00A01P3/00A01P21/00C12R1/07
CPCC12N1/20C12P17/10A01N63/00C12R2001/07C12N1/205A01N63/10
Inventor 谢学文李宝聚郑斐李磊石延霞柴阿丽
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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