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Bacillus subtilis and its application in control of stemphyllium botryosum of tomato

A technology of Bacillus subtilis and Puccinia leaf spot, applied in the fields of application, bacteria, and fungicides, can solve the problems of chemical resistance to environmental pollution and achieve the effect of easy action

Active Publication Date: 2019-09-03
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the resistance of tomato leaf spot and the serious environmental pollution caused by chemical control, it is of great significance to carry out research on the biological control of tomato leaf spot.

Method used

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  • Bacillus subtilis and its application in control of stemphyllium botryosum of tomato
  • Bacillus subtilis and its application in control of stemphyllium botryosum of tomato
  • Bacillus subtilis and its application in control of stemphyllium botryosum of tomato

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Example 1. Isolation, screening and identification of Bacillus subtilis ZF161

[0051] Strains were isolated from the rhizosphere soil collected from the greenhouses where tomato had been continuously planted for many years in Daxing District, Beijing. Pick single bacterium colony and cultivate in LB liquid medium, obtain bacterium suspension; Screen out from the bacterium colony that step (1) chooses with plate confrontation method (Stemphylium solani Weber) ) strains with antibacterial activity (Table 1). Finally, a strain named ZF161 was obtained.

[0052]Table 1 Screening the inhibitory effect of antagonistic bacterial strains on Puccinia solani

[0053] deal with concentration Colony diameter (mm) Bacteriostatic rate (%) IVF19 10 8 cfu / mL

23.00±0.41 70.51±0.93ab IVF43 10 8 cfu / mL

31.67±0.47 59.40±1.60d A-5 10 8 cfu / mL

31.00±2.27 60.26±5.67d ZF161 10 8 cfu / mL

19.00±1.08 75.64±2.60a 18051709-2-...

Embodiment 2

[0068] Embodiment 2, the preparation of bacillus subtilis (Bacillus subtilis) ZF161 bacterial suspension

[0069] A single colony of purified Bacillus subtilis ZF161 was inoculated in LB liquid medium, and cultured at 180 r / min at 28° C. for 2 days to produce a large number of Bacillus subtilis spores. Gained bacterium suspension contains the effective content of described Bacillus subtilis to be 10 7 ~10 9 cfu / mL.

Embodiment 3

[0070] Embodiment 3, the simple identification of the inhibitory action of Bacillus subtilis (Bacillus subtilis) ZF161 to tomato stolonifer leaf spot bacterium

[0071] Tomato solani leaf spot bacterium (Stemphylium solani Weber, provided by the Vegetable and Flower Research Institute, Chinese Academy of Agricultural Sciences) was received by the PDA plate center and cultivated for 24h, and the method of the embodiment of the present invention 1 was cultivated for 24 hours. Bacillus subtilis (Bacillus subtilis) ZF161 bacterial suspension was symmetrically inserted into the PDA plate in a square shape, 2.5cm away from the center, and three replicates and a blank control were set. Inhibition rate.

[0072] Inhibition rate=[(control colony diameter-treatment colony diameter) / (control colony diameter-stipe diameter)]×100%

[0073] The results are shown in Table 2 and Figure 5 , under the blank culture medium, the leaf spot fungus of Puccinia tomato grows rapidly, and the diamet...

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Abstract

The invention discloses Bacillus subtilis and its application in control of stemphyllium botryosum of tomato. The present invention provides Bacillus subtilis ZF161, which is preserved in the China Microbial Culture Collection Management Committee General Microbiology Center with a preservation number of CGMCC No. 16776. The Bacillus subtilis ZF161 provided by the invention is a soil rhizosphere microorganism of tomato, which is safe to humans, livestock and crops, and is environmentally friendly. The strain has a strong antagonistic effect on the stemphyllium botryosum of tomato. The biocontrol on the stemphyllium botryosum of tomato is carried out by using the strain, which is a highly effective and stable biological control method of stemphyllium botryosum of tomato, the strain has theadvantages of being easy to act on tomato plants and having strong antagonistic action against stemphyllium botryosum of tomato, and can better meet the requirements for controlling stemphyllium botryosum in tomato production.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a strain of bacillus subtilis and its application in the control of tomato peduncle leaf spot. Background technique [0002] Tomato lycopersici leaf spot can be caused by Stemphylium solani Weber and tomato lycopersici (Stemphylium lycopersici (Enjoji) Yamamoto). Tomato leaves, stems and fruits were extensively necrotic, resulting in reduced tomato yield. At present, chemical control methods are mainly used for the control of tomato stolonifer leaf spot. However, due to the resistance of tomato peduncle leaf spot and the serious environmental pollution caused by chemical control, it is of great significance to carry out research on the biological control of tomato peduncle leaf spot. [0003] At present, although chemical fungicides still play a dominant role in plant disease control, Bacillus-based biocontrol fungicides have gradually attracted att...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 谢学文李宝聚李新宇李磊石延霞柴阿丽
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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