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Streptomyces strain for preventing rice sheath disease and rice blast disease

A technology for rice sheath blight and rice blast, applied in the direction of bacteria, fungicides, and microbial-based methods, can solve the problems of pathogenic bacteria resistance, increased drug resistance, and environmental pollution

Inactive Publication Date: 2012-04-25
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since tricyclazole drugs are chemically synthesized drugs, although their application effect is good in the short term, if similar chemical agents are used continuously in a large area for a long time, it will seriously pollute the environment and lead to a substantial increase in the resistance and drug resistance of pathogenic bacteria.

Method used

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  • Streptomyces strain for preventing rice sheath disease and rice blast disease
  • Streptomyces strain for preventing rice sheath disease and rice blast disease
  • Streptomyces strain for preventing rice sheath disease and rice blast disease

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: the screening method of bacterial strain

[0036] Take 1 gram of turmeric rhizosphere soil from the turmeric planting site in Ya’an, Sichuan, and place it in a 250ml triangular flask filled with 60ml of 6% sterile peptone, cultivate it at 180rpm for 30min at 28°C, then take 1ml of the supernatant, and spread it on the ISP according to the gradient method -1 medium (tryptone 5.0g, yeast extract 3.0g, distilled water 1L, pH 7.0), cultured in a constant temperature incubator at 28°C for 2 days, picked a single colony, repeated purification before testing. The rice sheath blight pathogen and the rice blast pathogen were used as indicator strains, and the obtained single colony was used to carry out confrontation culture on the plate with the pathogen.

Embodiment 2

[0037] Embodiment 2: Antibiotic research of antagonistic bacteria

[0038] A. Determination of the teratogenic effect of antagonistic bacteria on sheath blight mycelia and rice blast: Inoculate antagonistic bacteria with the confrontation method, pick the sheath blight mycelium and normal mycelium at the edge of the confrontation and observe under an ordinary optical microscope , and examined with a scanning electron microscope.

[0039] It can be seen that the protoplasm of the control normal mycelium is uniform, the mycelium is round and strong, and the mycelium nodes are longer. The hyphae of sheath blight co-cultured with antagonistic bacteria shortened internodes, enlarged growth points, agglutinated protoplasts, enlarged mycelia tops, fractured mycelia, and severely twisted mycelia.

[0040] B, the influence of antagonistic strains on the germination of sheath blight sclerotia: insert the sterile glass slide into the melted sterile PDA medium, make it with a layer of me...

Embodiment 3

[0043] Embodiment 3: Identification of bacterial strain

[0044] A. Morphological characteristics: After culturing on sucrose Chapeauer's medium for 4 days at 28°C, conventional bacterial morphology was observed with an optical microscope and a scanning electron microscope.

[0045] B. Culture characteristics: sucrose Chase medium, potato medium, oatmeal medium, yeast maltose extract, inorganic salt starch medium, glycerol asparagine medium, tyrosine medium, cultured at 28°C for 7 days and observed Colony morphology and cell color.

[0046] C. Physiological and biochemical experiments: Physiological and biochemical identification of bacterial strains was carried out with reference to the contents of "Bergey's Bacterial Identification Manual (Eighth Edition)".

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Abstract

The invention discloses a Streptomyces griseus strain XZJ.SP12 which has strong antagonistic effect to pathogenic germs of rice sheath disease and rice blast disease by means of a method of inoculating points on a plate for prescreening. The strain is preserved in China Center for Type Culture Collection in Oct, 27th, 2009, with a preserving number of CCTCC No: M209236. The strain XZJ.SP12 has resistance to pathogenic germs of rice sheath disease and rice blast disease; seeondav metabolltes of the strain XZJ.SP12 can significantly inhibit growth of pathogenic germs of rice sheath disease and rice blast disease on the culture medium; in an infection test of rice sheath disease in fields, a control group has 67.8% infection index, however, the rice coated with the fermentation liquid of the strain has only 24.1% infection index, thereby showing that seeondav metabolltes of the strain XZJ.SP12 has a good resistance to the rice sheath disease and possesses an important intrinsic value to prevent rice diseases.

Description

technical field [0001] The present invention relates to a strain of Streptomyces, in particular to a Streptomyces strain XZJ which has a strong antagonistic effect on rice blast pathogens and rice blast pathogens through primary screening using Streptomyces screening technology. .SP12, the secondary metabolites of the strain have a good control effect on rice blast. Background technique [0002] Rice is the main food crop in my country. The planting area accounts for about 1 / 4 of the country's cultivated land area, and its annual output accounts for 1 / 2 of the country's total grain output. However, with the rapid development of economy, the gradual increase of population and the sharp decrease of arable land, coupled with the attacks of diseases and insect pests, rice production is facing increasing pressure. [0003] Rice blast (Pyricularia) and sheath blight (Thanatephorus cucumeris) are the two most harmful diseases in my country's rice fields. Rice blast is widely dist...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/545
Inventor 徐正君钟凯李利华高晓玲山口五十麿张小平高鸿徐永菊马炳田朱建清邓晓建姜凯王平荣黄静
Owner SICHUAN AGRI UNIV
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