Microbial fertilizer for increasing yield of ecological planting of potato and preparation method thereof
A technology of microbial fertilizer and ecological planting, which is applied in the field of microbial fertilizer and its preparation for improving the yield of potato ecological planting, can solve the problems of rampant damage of pathogenic bacteria, break the balance of flora, kill pathogenic bacteria, etc., and achieve growth promotion and improvement. Yield effect
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Embodiment 1
[0034] Present embodiment is a kind of preferred scheme of microbial fertilizer of the present invention and preparation method thereof:
[0035] Step 1: Cultivate Bacillus subtilis, Streptomyces aureus and Lactobacillus plantarum;
[0036] (1) Transfer Bacillus subtilis into the nutrient broth medium for shaking culture, the culture temperature is 37°C, the rotation number of the shake culture is 200rpm, the culture time is 36h, and then put into the refrigerator at 2-4°C Save for later use.
[0037] (2) transfer Streptomyces aureus into soybean meal powder liquid medium for shaking culture, the temperature of its cultivation is 28°C, the rotation number of its shaking culture is 200rpm, the time of its cultivation is 66h, and then put into 2 Stored in a -4°C refrigerator for later use; the above-mentioned soybean cake powder liquid medium for cultivating Streptomyces aureus is: 1 L of boiling water extract of 10% soybean cake powder, 10 g of glucose, and 2.5 g of NaCl.
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Embodiment 2
[0043] The present embodiment is another preferred version of the microbial fertilizer of the present invention and its preparation method:
[0044] Step 1: Cultivate Bacillus subtilis, Streptomyces aureus and Lactobacillus plantarum;
[0045] (1) Transfer Bacillus subtilis into the nutrient broth medium for shaking culture, the culture temperature is 30°C, the rotation number of the shake culture is 220rpm, the culture time is 48h, and then put into the refrigerator at 2-4°C Save for later use.
[0046] (2) transfer Streptomyces aureus into soybean meal powder liquid medium and carry out shaking culture, the temperature of its cultivation is 30 ℃, the revolution number of its shaking culture is 180rpm, the time of its cultivation is 60h, then put into 2 Store in a -4°C refrigerator for later use; the above-mentioned soybean cake powder liquid medium for cultivating Streptomyces aureus is: 1 L of boiling water extract of 10% soybean cake powder, 15 g of glucose, and 1 g of Na...
Embodiment 3
[0052] This example is a comparative experiment carried out in the field using the finished microbial fertilizer prepared in Example 2, and the comparison object is a commercially available chemical fertilizer.
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