Microbial organic fertilizer, and preparation method and purpose thereof

A technology of microbial organic fertilizer and preparation steps, which is applied in the field of agricultural biology, can solve the problems that the effect of disease prevention and control needs to be improved, and achieve the effect of improving the effect

Active Publication Date: 2014-02-19
珠海横琴协同投资管理有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Trichoderma can increase the number of beneficial microorganisms in the rhizosphere of crops and improve the structure of microbial flora in the rhizosphere of crops. As a component of microbial organic fertilizer, it can prevent and control diseases, but the effect of disease control still needs to be improved

Method used

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  • Microbial organic fertilizer, and preparation method and purpose thereof
  • Microbial organic fertilizer, and preparation method and purpose thereof

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preparation example Construction

[0015] The invention provides a preparation method of microbial organic fertilizer, which comprises the following steps: (1) a preparation step of chlamydospores, in which a Trichoderma strain is subjected to liquid submerged fermentation to produce chlamydospores Cystospores, wherein the Trichoderma strain is a strain of Trichoderma harzianum, preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC NO: 5812; (2) The preparation step of manure granules, In the step of preparing manure granules, feces, humic acid and water are mixed, inoculated with deodorizing bacteria for fermentation and deodorization, and then granulated and dried to obtain manure granules; (3) mixing step, in the mixing step, The manure particles were mixed with the chlamydospores.

[0016] Wherein, the physiological state of the Trichoderma strain is at least one of mycelia, chlamydospores and conidia.

[0017] Wh...

Embodiment 1

[0031] Get the potato 20g after peeling and boil for 20 minutes to get the juice, the juice is made into 100ml with 2g glucose, 1.5g agar and water, cool down after bottling and sterilize to obtain the PDA medium.

[0032] 195kg of starch, 130kg of yeast powder, 325kg of corn steep liquor, 19.5kg of CaCO 3 , 2.4kg (so that Zn 2+ 0.015% by weight) of ZnSO 4 , 6.5kg of MgSO 4 (so that Mg 2+ 0.02% by weight), add water to 6.5m 3 , mixed and sterilized to obtain a liquid medium.

[0033] Inoculate Trichoderma harzianum strain with preservation number CGMCC NO: 5812 in PDA medium and culture at 28°C for 6 days; inoculate the inoculated and cultivated PDA medium into shake flask liquid medium (100ml liquid medium inoculation 1 cm lawn grown on PDA medium 2 ), cultured for 24 hours at 28°C and 200rpm as the fermented seed tank inoculum; the cultured shake flask inoculum was inserted into the sterilized seed tank medium (inoculum size 2% (v / v)), under the conditions of 28°C an...

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Abstract

The invention provides a preparation method of a microbial organic fertilizer. The method comprises the following steps: (1) chlamydospore preparation by carrying out submerged fermentation on Trichoderma harzianum strains with preservation number of CGMCC NO:5812 to produce chlamydospores; (2) manure particle preparation by mixing manure, humic acid and water, and inoculating deodorizing bacteria for fermentation deodorization and granulating and drying to obtain the manure particles; and (3) mixing the manure particles and the chlamydospores. The invention also provides a microbial organic fertilizer obtained from the above preparation method. The invention also provides purpose of the microbial organic fertilizer in control of plant diseases. Through the above technical scheme, the invention can significantly improve the disease control effect of Trichoderma spp.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a microbial organic fertilizer, a preparation method of the microbial organic fertilizer and an application of the microbial organic fertilizer. Background technique [0002] Organic fertilizer is mainly composed of livestock and poultry manure, crop straw, humic acid and other organic matter. It can provide the nutrients needed for crop growth, improve the soil structure, and continuously release nutrients. The environment-friendly fertilizer. At the same time, organic fertilizer is also a medium that can provide good conditions for the reproduction of microorganisms, and is an important direction for the development of fertilizer science. [0003] Trichoderma (Trichoderma spp.) belongs to the subphylum Deuteromycotina, Hyphophyceae, Synchospora, Myxosporum, is a kind of ubiquitous saprophytic fungi, and is the most potential plant disease biological control strain amon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05F11/08
Inventor 陈舒
Owner 珠海横琴协同投资管理有限公司
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