A kind of biocontrol bacterial strain, bacterial strain screening method and application of controlling sesame fusarium wilt

A technology for sesame fusarium wilt and sesame fusarium wilt, which is applied in the field of biocontrol strains for preventing and treating sesame fusarium wilt, can solve problems such as insufficient control effect, threat, and lack of better prevention and control measures for sesame fusarium wilt, so as to improve disease prevention and control. control effect, reduce prevention and control costs, and achieve the effect of increasing farmers' income

Active Publication Date: 2016-11-30
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sesame Fusarium wilt often leads to immature sesame seeds, thin grains, and easy to blow off before harvest, which seriously threatens and hinders the development of sesame industry
[0003] Due to the narrow genetic basis of sesame germplasm resources, no sesame germplasm resources that are immune to Fusarium wilt have been found so far
At the same time, due to the low proportion of materials with high resistance to fusarium wilt, and the fact that sesame fusarium wilt is a soil-borne disease, the effect of agricultural control and chemical control is not obvious enough
So far, there are no good control measures for sesame fusarium wilt at home and abroad

Method used

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  • A kind of biocontrol bacterial strain, bacterial strain screening method and application of controlling sesame fusarium wilt
  • A kind of biocontrol bacterial strain, bacterial strain screening method and application of controlling sesame fusarium wilt
  • A kind of biocontrol bacterial strain, bacterial strain screening method and application of controlling sesame fusarium wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Isolation of pathogenic bacteria:

[0045] Take the affected plants of sesame wilt, cut the stem into 2cm pieces with a sterile cutting knife at the junction of sesame stem disease, disinfect the surface with 75% alcohol for 30 seconds, disinfect the surface with 3% sodium hypochlorite for 3 minutes, and then wash it with sterile water for 4 times ; use a sterile cutting knife to cut the small segment of the stem longitudinally in half from the middle, so that the vascular tissue is exposed, gently forceps the central vascular tissue, and place it on the PDA medium containing 50 μg / mL chloramphenicol; Cultivate at constant temperature and moisture for 4 days in a 27°C incubator, select dominant colonies and inoculate them on PDA for 4 days, then isolate and purify single spores, and store them for future use.

[0046] PDA medium: 200g potato, 20g glucose, 15g agar powder, 1000mL distilled water, pH value 7.0-7.2; sterilize with damp heat at 121°C for 20min.

Embodiment 2

[0048] Isolation of soil microorganisms:

[0049] Take 1g of soil from the roots of strong sesame seeds in the sesame field in Fenyang City, Shanxi Province, and dilute it to 10% with sterile water. -5 ~10 -3, spread the diluted mixture evenly on 1 / 10 TSA medium, and incubate it upside down in a constant temperature incubator at 28°C for 2 days. Bacteria were separated and purified by streaking on the surface of TSA medium, and the purified bacteria were mixed with 50% glycerol in equal amounts, and stored in a -70°C freezer for future use.

[0050] 1 / 10 TSA medium: 3 g of TSB (Trypticase Soya Broth, Difco.), 15 g of agar, 1000 mL of distilled water, pH 7.0-7.2; sterilized by moist heat at 121 °C for 20 min.

[0051] TSA medium: 30g of TSB (Trypticase Soya Broth, Difco.), 15g of agar, 1000mL of distilled water, pH 7.0-7.2; sterilized at 121°C for 20min.

Embodiment 3

[0053] Screening of biocontrol strains:

[0054] The screening of biocontrol strains adopts the plate confrontation culture method. The Fusarium oxysporum block is placed in the center of the PDK medium, and the soil microorganisms are tested at a distance of 25mm from the block. They are placed in a 27°C incubator for 5 days to observe whether there is an inhibition zone. And measure the size of the inhibition zone, such as figure 1 shown. A biocontrol strain of sesame fusarium wilt was found in the antagonistic test, and it was named biocontrol strain SF4-24.

[0055] PDK medium: 200g potato, 20g glucose, 10g peptone, 15g agar, 1000mL distilled water, pH 7.0-7.2; sterilize at 121℃ for 20min.

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Abstract

The invention discloses a biocontrol bacterial strain for preventing and treating sesame fusarium wilt, a strain screening method and application thereof, and belongs to the technical field of sesame disease prevention and control. The biocontrol strain is Bacillus subtilis, preservation number: CCTCC NO: M2013572, preservation unit: China Collection Center for Typical Cultures, preservation address: Wuhan University Collection Center, Luojia Mountain, Wuchang, Wuhan City, Hubei Province, preservation date: November 14, 2013. The invention separates and purifies the sesame root soil to obtain the biocontrol bacterial strain capable of preventing and treating the sesame wilt, and has the effect of promoting the growth of the sesame plant. The biocontrol bacterium agent prepared by the bacterial strain is a biological agent and will not pollute the environment and ecology. Using the bacterial agent to treat sesame seeds or plants can effectively reduce the consumption of other chemical pesticides, reduce the cost of prevention and control of sesame diseases, and have the function of increasing sesame production.

Description

technical field [0001] The invention relates to a biocontrol bacterial strain for preventing and treating sesame fusarium wilt, as well as a screening method and application of the bacterial strain, and belongs to the technical field of sesame disease prevention and control. Background technique [0002] Sesamum (Sesamum indicum L., 2n=26), belonging to the genus Linaceae, is one of the oldest oil crops in the world and an important high-quality oil crop in my country. The annual planting area of ​​sesame in my country is about 12 million mu, and the total output is about 750,000 tons, which plays an important role in the international oil trade. Sesame Fusarium wilt (SFW) is a soil-borne fungal disease caused by Fusarium oxysporum Schl.f.sp.sesami (Zap.) infection. Sesame can be infected by Fusarium oxysporum during the whole growth period. The disease is a worldwide disease, and occurs to varying degrees in sesame-producing countries such as India, Sudan, Egypt, and Paki...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/125
Inventor 苗红梅张海洋常淑娴段迎辉魏利斌李春张体德李春明
Owner HENAN ACAD OF AGRI SCI
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