Trichoderma strain and purpose thereof, and chlamydospore and preparation method thereof

A technology of Trichoderma and strains, applied in the field of agricultural biology, can solve the problems that the effect of disease prevention and control needs to be improved, and achieve the effect of improving the effect

Active Publication Date: 2015-06-10
珠海横琴协同投资管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Trichoderma can increase the number of beneficial microorganisms in the rhizosphere of crops, improve the microbial flora structure in the rhizosphere of crops, and play a role in disease control, but the effect of disease control still needs to be improved

Method used

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  • Trichoderma strain and purpose thereof, and chlamydospore and preparation method thereof
  • Trichoderma strain and purpose thereof, and chlamydospore and preparation method thereof

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preparation example Construction

[0018] The present invention also provides a method for preparing the chlamydospore of the bacterial strain, the method comprising: performing submerged fermentation on the above-mentioned Trichoderma strain to produce the chlamydospore.

[0019] Wherein, the operation of the liquid submerged fermentation can be carried out according to the conventional liquid submerged fermentation method, preferably, the operation of the liquid submerged fermentation includes the following steps: (1) Inoculation step, in the inoculation step, the above-mentioned Inoculate the conidia and / or mycelium of the above-mentioned Trichoderma strain into the liquid medium; (2) fermentation step, in the fermentation step, inoculate the conidia and / or mycelium of the Trichoderma strain The liquid medium is fermented in a fermenter.

[0020] Wherein, the conidia and / or mycelium of the Trichoderma strain can be obtained using a conventional slant culture method, for example, the mycelium of the Trichoder...

Embodiment 1

[0034] Get the potato 20g after peeling and boil for 20 minutes to get the juice, the juice is made into 100ml with 2g glucose, 1.5g agar and water, cool down after bottling and sterilize to obtain the PDA medium.

[0035] 195kg of starch, 130kg of yeast powder, 325kg of corn steep liquor, 19.5kg of CaCO 3 , 2.4kg of ZnSO 4 (so that Zn 2+ 0.015% by weight), 6.5kg of MgSO 4 (so that Mg 2+ 0.02% by weight), add water to 6.5m 3 , mixed and sterilized to obtain a liquid medium.

[0036] The Trichoderma harzianum strain with the preservation number CGMCC NO: 5812 was inoculated in PDA medium and cultured at 28°C for 6 days; the inoculated and cultivated PDA medium was excavated and inoculated into shake flask liquid medium (100ml liquid medium Inoculate 1 cm of bacterial lawn grown on PDA medium 2 ), cultured for 24 hours at 28°C and 200rpm as the fermented seed tank inoculum; the cultured shake flask inoculum was inserted into the sterilized seed tank medium (inoculum size 2...

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Abstract

The invention discloses a Trichoderma strain of Trichoderma harzianum, and the strain is preserved in General Microbiological Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CGMCC No.5812. The invention also provides the purpose of the Trichoderma strain in prevention and treatment of plant diseases. The invention also provides a preparation method of chlamydospores. The method includes submerged fermentation on the above Trichoderma strain to produce chlamydospores. The invention also provides the chlamydospores obtained by the above preparation method. Through the above technical scheme, the invention can significantly improve the disease control effect of Trichoderma.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a Trichoderma strain, the application of the Trichoderma strain, a chlamydospore and a preparation method of the chlamydospore. Background technique [0002] Trichoderma (Trichoderma spp.) belongs to the subphylum Deuteromycotina, Hyphophyceae, Synchospora, Myxosporum, is a kind of ubiquitous saprophytic fungi, and is the most potential plant disease biological control strain among fungi , can be used to control a variety of plant fungal diseases. Common species of Trichoderma include Trichoderma harzianum (T.harzianum), Trichoderma harsei (T.hamatum), Trichoderma polysporum (T.polysporum), Trichoderma koningii (T. Mold (T.pseudokoningii), Trichoderma viride (T.viride) and Trichoderma longibrachiatum (T.longibrachiatum), etc. Among many species, Trichoderma viride and Trichoderma harzianum are particularly outstanding in terms of plant disease control and control effec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12N3/00C12R1/885
Inventor 陈舒
Owner 珠海横琴协同投资管理有限公司
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