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Streptomyces albidoflavus and applications thereof in microbial fertilizers

A technology of Streptomyces albicans and microbial fertilizer, which is applied in the field of microorganisms and can solve the problems of poor fermentation performance of strains and poor field test results

Active Publication Date: 2016-08-24
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these soil fungal disease biocontrol strains have strong antifungal activity, the field test effect is poor, and many strains have poor fermentation performance, and there are usually certain geographical limitations in application

Method used

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  • Streptomyces albidoflavus and applications thereof in microbial fertilizers
  • Streptomyces albidoflavus and applications thereof in microbial fertilizers
  • Streptomyces albidoflavus and applications thereof in microbial fertilizers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Isolation, identification and biological characteristics of strains

[0049] 1. Isolation and purification of strains

[0050] In this experiment, several strains of actinomycetes were isolated from marine activated sludge. The process is as follows: add 1g of sample to a sterilized 300ml Erlenmeyer flask filled with small glass beads and 99ml of sterile saline, and incubate on a shaker at 28°C for 1h . Take 1 ml suspension and dilute to 10 by gradient dilution method -4 , take stock solution, 10 -2 Diluent and 10 -4 The dilution solution was coated with potassium dichromate at a final concentration of 50 μg / ml Gaoshi No. 1 medium, and single colonies were picked on the 5th, 7th, and 10th days, and then streaked on the PDA medium. for purification and isolation.

[0051] The resulting purified strain was stored at -80°C with 20% glycerol.

[0052] 2. Identification of strains

[0053] 1. Morphological identification

[0054] For the morphological identification ...

Embodiment 2

[0070] Antifungal Activity Test of Streptomyces albus W68 Isolated from Deep Sea Sludge

[0071] Determination of antifungal activity of Streptomyces microalbicans W68 by plate confrontation method:

[0072] (1) Streptomyces cultivation and collection: use the streaking method to inoculate Streptomyces microalbicans Streptomyces microalvanus W68 on a PDA plate, culture at 28°C for 5-7 days until sufficient spores are produced; scrape the spores into appropriate amount of sterile water to prepare The spore suspension is ready for use.

[0073] (2), the cultivation of pathogenic bacteria: inoculate cucumber blight, cabbage wilt, wheat root rot, rice blast fungus, cotton fusarium wilt, and cotton Verticillium dahliae on the PDA plate, and use it after cultivating for one week at 28°C.

[0074] (3), plate stand-off test: respectively inoculate the bacterial block of pathogenic bacteria culture in the center of the PDA plate, inoculate 5 ul of Streptomyces microalbicans W68 spore ...

Embodiment 3

[0076] Streptomyces albus W68 as the material microbial agent:

[0077] (1) Preparation of liquid seeds

[0078] Pick the aerial hyphae and spores of Streptomyces alboflavinus W68 grown on the PDA plate for 4-5 days, inoculate them into 2×YT liquid medium, culture at 28-30°C, 180rpm for 1-2 days, and then use it as a seed solution .

[0079] (2) Preparation of solid fermented seeds

[0080] The liquid seeds are inserted into the tissue culture bottle equipped with the solid fermentation material according to the mass ratio of 10%, and cultivated for 4-6 days at 28-30 DEG C to obtain the solid fermentation seeds.

[0081] (3) Microbial agents

[0082] Using agricultural waste, livestock and poultry manure, edible fungus residue, bran or soybean meal as basic raw materials, add a certain proportion of MnSO 4 , (NH) 2 SO 4 , The ratio of material to water is 1:1. Inoculate solid fermented seeds according to the ratio of 10%. Cultivate at 28-30°C for 5-7 days, air-dry afte...

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Abstract

The invention discloses streptomyces albidoflavus W68 (with the preservation number CGMCC NO.12210) having wide-spectrum antifungal activity and being separated from deep-sea sludge and application technologies of microbial agents prepared by adopting streptomyces albidoflavus W68 and microbial fertilizers prepared by taking streptomyces albidoflavus W68 as a material. The application technologies of the microbial agents prepared by adopting streptomyces albidoflavus W68 separated from the deep-sea sludge and the microbial fertilizers prepared by taking streptomyces albidoflavus W68 as the material are simple, convenient and efficient, and are environmentally friendly, and the spore concentration of the antifungal preparation obtained through solid fermentation can achieve tens of billions. The pot experiment proves that streptomyces albidoflavus W68 separated from the deep-sea sludge has significant prevention and treatment effects for the soil-borne fungal diseases including wheat root rot, melon fusarium wilt, phytoph-thora capsici leonian and wheat take-all. Streptomyces albidoflavus W68 has great application prospect in the pollution-free prevention and control for the soil-borne fungal diseases.

Description

technical field [0001] The invention relates to a marine-derived Streptomyces albidoflavus strain W68 with broad-spectrum antifungal activity and biocontrol application potential, belonging to the application of marine microorganisms and the field of microbial technology such as crop disease prevention and control. Background technique [0002] Fungal diseases are the main cause of crop yield and quality reduction. The frequent use of chemical pesticides has led to a sharp increase in the resistance of some pathogenic fungi, pollution of the environment and agricultural products, and damage to the balance of microorganisms in and out of plants, which has exacerbated the outbreak of diseases. [0003] The research on the prevention and control of soil-borne plant fungal diseases has always been a hot and difficult point, and the fungicides on the market generally have no or little effect on it. Over the years, people have also screened some biocontrol bacteria resistant to s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C05F15/00C12R1/465
CPCA01N63/00C05F3/00C12N1/205C12R2001/465C05F11/00C05F11/08
Inventor 李少杰张振颖孙宪昀
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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