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Bacillus amyloliquefaciens and inocula, inocula preparation method and application thereof

A technology of amylolytic bacillus and bacillus, applied in the field of microorganisms, can solve the problems of low survival rate, inability to survive, unstable control effect, etc., and achieve a strong killing effect

Active Publication Date: 2018-04-06
ANHUI LIUGUO CHEM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although biological control has received more and more attention, it is still relatively small that can be actually applied to the control of plant diseases. The microorganisms with good biocontrol effects isolated in the laboratory may appear in the application process due to environmental factors or The cause of the bacteria itself is that the survival rate in the soil is low or cannot survive, and its control effect is unstable

Method used

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  • Bacillus amyloliquefaciens and inocula, inocula preparation method and application thereof
  • Bacillus amyloliquefaciens and inocula, inocula preparation method and application thereof
  • Bacillus amyloliquefaciens and inocula, inocula preparation method and application thereof

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Embodiment 1A

[0036] Isolation, identification and preservation of embodiment 1AMCC100200 bacterial strain

[0037] 1.1 Isolation of AMCC100200 strain

[0038] A strain with broad-spectrum resistance to plant diseases was screened from the rhizosphere soil of plants, named AMCC100200 strain, and the specific isolation method was as follows:

[0039] Take 10 g of plant rhizosphere soil, put it into a 250 mL Erlenmeyer flask filled with sterile glass beads and 90 mL of sterile water, and shake well. Place the shaken Erlenmeyer flask in a shaker at 37°C and shake at 180rpm for 30min. After taking it out, carry out gradient dilution on the ultra-clean bench to obtain 10 -3 Gradient, 10 -4 Gradient, 10 -5 Gradient, 10 -6 Gradient and 10 -7 Gradient dilutions. The dilutions of these several gradients were spread on the LB medium plate respectively, and 6 parallels were set for each dilution gradient, sealed with a parafilm and then placed in a 37°C incubator for cultivation. After 24 hour...

Embodiment 2

[0047] The preparation of embodiment 2 bacterial agents

Embodiment 2-1

[0049] S1. Strain activation: Streak inoculation of Bacillus amyloliquefaciens AMCC100200 on LB solid medium plate, and culture at 37°C for 24 hours;

[0050] S2. Seed solution preparation: Inoculate the activated Bacillus amyloliquefaciens AMCC100200 in a 5% inoculation amount into a conical flask equipped with LB liquid medium, and vibrate in a constant temperature oscillator at 37° C. and 180 rpm for 24 hours to obtain a seed solution;

[0051] S3, seed tank fermentation culture: the seed solution in step S2 was inoculated into a 50L seed tank with an inoculation amount of 5%, and the liquid medium in the seed tank was filled with a liquid volume of 30L, and cultured at 37°C for 8h to obtain a fermentation culture;

[0052] S4, fermentation culture: the fermentation culture in the step S3 is inoculated in the 500L fermenter with the inoculum amount of 5%, and the liquid culture medium filling capacity in the fermenter is 350L, cultivates at 37 ℃ for 24h, obtains Bacillus amy...

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Abstract

The invention relates to bacillus amyloliquefaciens and inocula, an inocula preparation method and an application thereof. The bacillus amyloliquefaciens AMCC100200 is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No.14170. The inocula of bacillus amyloliquefaciens is obtained through spray drying of bacteria liquid of bacillus amyloliquefaciens AMCC100200. The bacillus amyloliquefaciens AMCC100200 has strong inhibition effect on a plurality of plant pathogens. The field trial result shows that after the inocula of the bacillus amyloliquefaciens AMCC100200 is used for processing, cucumber anthracnose condition index of the plant is decreased by 74.41%, and the tomato root knot nematode disease condition index is decreased by 65%.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of bacillus amyloliquefaciens and its bacterial agent, a preparation method and application of the bacterial agent. Background technique [0002] Plant diseases are one of the main factors affecting high and stable agricultural yields and high quality. The pathogens mainly include fungi, bacteria, nematodes and viruses. For example, Fusarium oxysporum is a soil-borne fungal disease, which can cause the occurrence of hundreds of plant wilts such as melons and nightshades. It occurs widely all over the country, with a general incidence rate of 10% -30%, and it can reach more than 50% in severe cases. , with the gradual expansion of the planting area of ​​melons, Fusarium wilt has become one of the most important factors restricting the production of melons; Worldwide, annual losses from plant-parasitic nematodes amount to $100 billion, with root-knot nematode...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P5/00A01P3/00A01G13/00C12R1/07
CPCA01G13/00A01N63/00C12N1/20C12R2001/07C12N1/205
Inventor 周波沈浩王建宇郑之银刘荣张俊孙诚李婷许琦嫚彭克荣盛子璜张红星朱广飞陈晨童荟梁靖
Owner ANHUI LIUGUO CHEM CO LTD
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