Continuous-cropping bacteria resistant agent
A technology of stubble bacteria agent and bacteria residue, which is applied in the field of anti-cropping bacteria agent, and can solve the problems of low number of viable bacteria, high energy consumption, and inconspicuous effect
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Embodiment 1
[0102] Embodiment 1: Preparation of the anti-cropping bacterial agent of the present invention
[0103] The implementation steps of this embodiment are as follows:
[0104] First, prepare the Pythium oligandrum ferment
[0105] A. Preparation of CMA medium
[0106] Boil 60g corn kernels in 1000mL distilled water for 90min, filter with double-layer gauze, add 20g glucose to the obtained filtrate, then add water to make up to 1000mL, subpackage, and sterilize at a temperature of 121°C for 20min to obtain the CMA Culture medium;
[0107] Inoculate 0.1 g of Pythium oligandrum provided by Beijing Biorui Biotechnology Co., Ltd. into 200 mL of CMA liquid medium, and carry out shaking culture in a shaking culture shaker at a temperature of 28 ° C and a rotation speed of 180 r / min for 4 days;
[0108] B. Activation culture
[0109] 200g of potatoes, 20g of glucose, 15-20g of agar and 1000ml of tap water were used to prepare PDA medium by conventional methods.
[0110] Use a steril...
Embodiment 2
[0120] Embodiment 2: Preparation of the anti-cropping bacterial agent of the present invention
[0121] The implementation steps of this embodiment are as follows:
[0122] First, prepare the Pythium oligandrum ferment
[0123] A. Preparation of CMA medium
[0124] Boil 60g corn kernels in 1000mL distilled water for 90min, filter with double-layer gauze, add 20g glucose to the obtained filtrate, then add water to make up to 1000mL, subpackage, and sterilize at a temperature of 121°C for 20min to obtain the CMA Culture medium;
[0125] Inoculate 0.1 g of Pythium oligandrum provided by Beijing Jinjiufeng Technology Development Co., Ltd. into 200 mL of CMA liquid medium, and carry out shaking culture in a shaking culture shaker at a temperature of 28 ° C and a rotation speed of 180 r / min for 4 days;
[0126] B. Activation culture
[0127] PDA medium was prepared in the same manner as in Example 1.
[0128] Use a sterile inoculation loop to pick a small amount of mycelium fro...
Embodiment 3
[0138] Embodiment 3: preparation of the anti-cropping bacterial agent of the present invention
[0139] The implementation steps of this embodiment are as follows:
[0140] First, prepare the Pythium oligandrum ferment
[0141] A. Preparation of CMA medium
[0142] Boil 60g corn kernels in 1000mL distilled water for 90min, filter with double-layer gauze, add 20g glucose to the obtained filtrate, then add water to make up to 1000mL, subpackage, and sterilize at a temperature of 121°C for 20min to obtain the CMA Culture medium;
[0143] Inoculate 0.1 g of Pythium oligandrum provided by Beijing Biorui Biotechnology Co., Ltd. into 200 mL of CMA liquid medium, and carry out shaking culture in a shaking culture shaker at a temperature of 28 ° C and a rotation speed of 180 r / min for 4 days;
[0144] B. Activation culture
[0145] PDA medium was prepared in the same manner as in Example 1.
[0146] Use a sterile inoculation loop to pick a small amount of mycelium from the culture...
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