59 results about "Pythium oligandrum" patented technology

The invention relates to a composite microbial bacterial fertilizer with functions of disease prevention, growth promotion and yield increase used in the crop cultivation process and application thereof. The composite microbial bacterial fertilizer comprises a fungicidal composition consisting of two microbial fungicidal strains such as pythium oligandrum and trichoderma harzianum, wherein the spore number ratio of the pythium oligandrum to trichoderma harzianum is (1:4)-(1:2). Based on the biological control principle of preparing bacteria through bacteria and the drug-fertilizer dual-effect idea, the pythium oligandrum and trichoderma harzianum with high-efficiency hyperparasitism effect on the soil-borne pathogen and a bio-fertilizer containing humic acid, amino acids and the like are proportioned and mixed, the composite microbial bacterial fertilizer with the disease-resistant and nutritive effects is developed, the functions of inhibiting and killing bacteria and increasing crop nutrition are integrated, the fertilizer is conveniently used, the manual cost is saved, and the yield and effect are increased; and moreover, the fertilizer has obvious prevention and control effects on strawberry soil-bore diseases.

The invention discloses a whole-course biological control method of tobacco diseases and insect pests. The method comprises the following steps: 1) in the seedling stage: using predatory mites for controlling thrips and tomato spotted wilf viruses; using harmonia axyridis and aphidius gifuensis for controlling myzus persicae and the thrips; using penicillium to inactivate mycelia, and 0.5% of chitosan and 0.5% to 2% of amino-oligosaccharin for controlling tobacco mosaic diseases; 2) in the field period: using the predatory mites and harmonia axyridis for controlling the thrips, the tomato spotted wilf viruses and the myzus persicae; using sex attractants for controlling prodenia litura adults and helicoverpa assulta adults; using Cahtheconidea furcellata for controlling prodenia litura larvae; using 0.5% of chitosan and 0.5% to 2% of amino-oligosaccharin for controlling the tobacco mosaic diseases; using Holder Burkholderia, and Pythium oligandrum for controlling tobacco black shank, black root rot, root knot nematode diseases and the like. The whole-course biological control method of the tobacco diseases and insect pests disclosed by the invention thoroughly eliminates pollution of chemical pesticides on the environment and tobacco pesticide residues and effectively controls the diseases and insect pests continuously and effectively, and the loss of tobacco quality for the diseases and insect pests is controlled within 5%.

The invention discloses a broad-spectrum composite microbial fungicide and a preparation method thereof. Original composite conidial powder consists of the following three microorganisms: bacillusthuringiensis GSICC32807, bacillus amyloliquefaciens GSICC32826 and pythiumoligandrum GSICC61615. Two types of bacterial spore powder and a type of fungal spore powder are mixed according to the weight ratio of 1:1:1 to prepare a broad-spectrum composite microbial fungicide product. The broad-spectrum composite microbial fungicide product provided by the invention can be quickly planted around the root of a plant to obviously prevent, resist and kill microbial diseases of various crops by competing infection sites with pathogenic bacteria, implementing the hyperparasitism action, secreting auxin, promoting nutrient absorption of the plant and the like; furthermore, the road-spectrum composite microbial fungicide has the effects of promoting the growth of the crops, increasing the yield and improving the quality.

Disclosed is a continuous-cropping bacteria resistant agent. The continuous-cropping bacteria resistant agent is composed of bacillus licheniformis bacteria powder, streptomyces microflavus powder, pythium oligandrum yeasts, green wooden mold powder, edible fungi residues, compound sodium nitrophenolate and alpha-naphthalene acetic acid. By the arrangement, continuous-cropping diseases of crops can be alleviated, diseases of bacterial wilt, tobacco mosaic and the like caused by continuous cropping are highly prevented, various diseases of thielariopsis basicola, blight, root-knot nematode, damping off and the like can be prevented, and incidence rate and disease index of flue-cured tobacco black shank disease, the thielariopsis basicola and common mosaic are effectively reduced; by the aid of the continuous-cropping bacteria resistant agent, flue-cured tobacco can be promoted in early growth and quick tiller, growth period is brought forward, plant height and stem thickness of the flue-cured tobacco are increased, yield of flue-cured tobacco leaves is increased, and appearance quality of the tobacco leaves is improved.

The invention relates to novel soil conditioner which mainly comprises actinomycetes nitrogen-fixing bacteria, phosphobacteria, potassium bacteria, bacilli and pythium oligandrum and additionally comprises fulvic acid, organic fertilizer and medium trace elements. The novel soil conditioner has the following efficacies: through the soil application, rapidly regulating the chemical, physical, organic and biological fertility of the soil, providing excellent soil environment for the production of crops, and having excellent effect on rapid seedling recuperation, seedling death reduction, healthy and strong growth, and improvement in crop immunity after crop planting or transplanting, so as to lay a solid foundation for high yield and realizing sustainable and sound development of agriculture. The final product of the novel soil conditioner is power.

The invention relates to a method for preparing a pythium oligandrum fermentation liquor and a method for effectively controlling tobacco black shank by utilizing the fermentation liquor, wherein a 'polyversum. pythium oligandrum' oospore preparation (strain: pythium oligandrum DV74) is utilized to obtain pythium oligandrum strains, and the pythium oligandrum fermentation liquor is prepared through liquid fermentation, filter concentration and other steps. Compared with a pythium oligandrum oospore preparation, the pythium oligandrum fermentation liquor has good and stable effect on controlling the tobacco black shank, is slightly influenced by the environmental conditions, and has a simple application method and strong practicability; the cost is low, and the application cost is only 20%of the pythium oligandrum oospore preparation; and pythium oligandrum fermentation equipment is simple and universal, less resources are consumed, the industrial production requirement is low, and a common microbial fermentation factory can satisfy the production.

The invention relates to an inducer for breaking dormancy of pseudo-ginseng root tuber and a preparation method and an application thereof. The inducer is composed of the following raw materials, by weight, 1.3-1.6 parts of spermidine, 1.8-2.2 parts of spermine, 0.0008-0.0012 part of pythium oligandrum, 0.6-1 part of potassium metasilicate, 0.05-0.15 part of sodium selenite, 0.005-0.01 part of brassinolide, 8-10 parts of organic zinc, 0.1-0.2 part of inositol and 10000 parts of water. In late October and in early November, when pseudo-ginseng plant leaves grow old before beginning to show a sign of yellowing, the inducer is used to spray pseudo-ginseng plants and fibrous roots. Each pseudo-ginseng plant and the fibrous root are sprayed with 5-10 ml of the inducer, and each plant is sprayed for one time. The inducer provided by the invention has advantages of fast effect, short time, high emergence rate, no side-effect, low dosage, convenience in operation and the like.

The invention discloses an application of a microbial agent in seedling raising of Angelica sinensis, and a prevention and treatment method for soil-borne diseases. The microbial agent provided by the invention comprises three self-prepared microbial agents and a commercial microbial agent which are respectively used for seed dressing of Angelica sinensis and seedling raising in an uncultivated land; and through counting of the weight of an Angelica sinensis seedling, the number of Angelica sinensis plants and the level of Angelica sinensis seedlings in a test plot of Angelica sinensis seedlings, feasibility of the application the microbial agent in seedling raising of Angelica sinensis and prevention and treatment effects on the soil-borne diseases are researched. A composite fungal agent (Pythium oligandrum and Beauveria bassiana, PO+BB) provided by the invention has best effects on seedling raising of Angelica sinensis and the prevention and treatment of the soil-borne diseases, and is superior to the commercial fungal agent Polyversum Pythium oligandrum oospores; and the composite fungal agent has good application prospects in agriculture and production of traditional Chinese medicinal materials.

The invention relates to a biological repairing agent of soil. The biological repairing agent is prepared from bacillus cereus bacterium powder, pythium oligandrum bacterium powder, trichoderma viride bacterium powder, a sphingobacterium multivorum fermented product, mushroom dreg of edible mushrooms, calcium amino acid chelate, zinc amino acid chelate and DA-6; the biological repairing agent can be used for degrading pollution of petroleum agriculture and pesticide residues, and can be applied to a farmland so that the aims of ameliorating soil and preventing diseases, reducing the residues and promoting growth so as to improve the micro-ecological activity and self-repairing capability of the soil, increase the yield of crops, improve the quality and maintain the agricultural ecological health can be realized.

The invention provides an agrobacterium-mediated pythium oligadrum gene transformation method. The agrobacterium-mediated pythium oligadrum gene transformation method comprises the following steps that 1, a pythium oligadrumsuspension is prepared; 2, an agrobacterium solution containing a target gene carrier is prepared; 3, the agrobacterium solution infects the pythium oligadrum suspension; 4, culture is recovered; 5 converter screening is performed. The agrobacterium-mediated pythium oligadrum gene transformation method adopts a liquid oscillation and co-culture mode to improve the transformation rate, the transformation rate is improved in a liquid culture recovery mode, the operating process is simplified, the pollution rate is reduced, and resistant single colonies can be conveniently selected for purified culture and identification. The agrobacterium-mediated pythium oligadrum gene transformation method is simple, convenient to operate, efficient and stable, and a research way is provided for transformation by utilizing functional genes and their properties of biological control fungi.

The present invention provides a freeze-dried powdered, dried and suspended fungicide and a preparation method thereof. The fungicide consists of the following raw materials in percentages by weight: 15-30% of pythium oligandrum oospore powder and fermented liquid freeze-dried powder, 9-18% of wetting and dispersing agent, 0.2-0.5% of ultraviolet protective agent, 3-12% disintegrant, 3.0-10% of binder and 29.5-69.8% of carrier. All the raw materials are mixed, mist sprayed and granulated to obtain the finished product. The fungicide makes up the drawbacks of using pythium oligandrum oospore powder and pythium oligandrum fermented liquid freeze-dried powder individually, this dosage form improves the germination rate and suspension rate of oospores, and the fungicide is fast in wetting and disintegrating speed, excellent in dispersion and thermal storage stability, and provides a new material for plant disease prevention and cure.

The invention discloses a preparation method and an application of Pythium oligandrum microbial fertilizer. The preparation method comprises steps as follows: firstly, a Pythium oligandrum strain or a Pythium oligandrum oospore is subjected to solid propagation, and a propagated strain is obtained; the propagated strain is subjected to liquid fermentation, a fermentation broth is obtained and evenly mixed with a liquid medium used for liquid fermentation, and a mixed liquid is obtained; the mixed liquid is mixed with a crushed and sterilized carrier material, a mixture is obtained, covered with a plastic film and composted for 20-30 d, and the Pythium oligandrum microbial fertilizer is prepared. The Pythium oligandrum microbial fertilizer can promote growth of crops, vegetables and fruit trees, promote the plant growth amount to increase by 7.0% generally, can control about 60% of plant fungal diseases caused by pathogenic fungi such as phytophthora, botrytis cinerea, verticillium, alternaria, botrytis, rhizoctonia, pythium and the like and has growth promoting and disease preventing effects superior to those of existing oospore preparations.

The invention discloses a biocontrol pythium oligandrum strain for preventing and controlling pythium root rot of seedlings of cucumis sativus L., gray blight of cucumis sativus L. and meloidogyneoryzae diseases and a separation and application method thereof, and belongs to the technical field of crop disease prevention and control. The biocontrol strain is pythium oligandrum GAQ1, and the preservation number is CGMCC No.17470. The invention further discloses the separation method of the biocontrol pythium oligandrum and the application of the biocontrol pythium oligandrum in preventing and treating oomycete diseases of cucumis sativus L. and meloidogyneoryzae diseases. The pythium oligandrum CGMCC 17470 can significantly inhibit the infection of crops by pythium oxysporum, phytophthora capsici and meloidogyneoryzae. The strain GAQ1 is isolated from the soil and harmoniously integrated with the ecological environment, has no toxicity or pathogenicity, and can be prepared into a biological preparation for biological prevention and control over plant diseases.

The invention provides a preparation and regeneration method of pythium oligandrum protoplasts. The reparation and regeneration method comprises the following steps: a, preparing thallospore hybrids; b, preparing protoplasts; c, purifying the protoplasts; and d, regenerating the protoplasts. According to the method, a complex enzyme system containing lysing enzyme, cellulsase and lyticase is adopted for carrying out enzymolysis on the mycelia of pythium oligandrum, then the pythium oligandrum protoplasts are obtained, and the protoplasts are regenerated through liquid shaking culture adopting a regeneration culture medium taking mannitol as an osmotic pressure stabilizer. The number of the pythium oligandrum protoplasts prepared by adopting the method is large, the yield is high, and the activity is good; through the liquid culture regeneration path, relatively high regeneration rate can be obtained, and the regeneration rate can achievce 1.25%. The method is easy and convenient to operate and good in stability, the obtained pythium oligandrum protoplasts can completely satisfy the research and application such as late gene genetic transformation and cell fusion. The invention further provides composite enzymatic hydrolysate for preparing the pythium oligandrum protoplasts, the composite enzymatic hydrolysate is a key factor in the preparation process of the pythium oligandrum protoplasts, and the enzymatic hydrolysate is suitable for being further developed and applied.

The invention belongs to the technical field of agricultural planting and discloses a planting method of high-yield papaya. The planting method comprises the following steps: (1) covering planting ground with nutrient matrix; (2) selecting cuttings and peeling the ends of the cuttings and soaking in 0.01% indoleacetic acid aqueous solution, and soaking in rooting solution after washing, and finally soaking in NAA solution; (3) inserting the treated cuttings into the culture medium, building a shed, applying with compound organic fertilizer once every 10 days, and spraying with 600 times liquidof pythium oligandrum wettable powder every week; and (4) carrying out a first fertilization with compound fertilizer after the cuttings transplantation, spraying nutrient solution on leaves one weekafter the first fertilization, carrying out root topdressing 40-60 days after transplantation, and killing insect pests with an insecticide of controlling diseases and insect pests. The papaya planted the method has high survival rate of the cuttings, the yield of the papaya per mu is significantly increased, the nutrient value of the papaya is increased, and the pesticide residues are low.

The invention relates to a pythium oligandrum spore production inducer, a pythium oligandrum spore production inducing medium containing the inducer, and a preparation method and application of the pythium oligandrum spore production inducing medium. By adding the spore production inducer, the pythium oligandrum mycelium can quickly produce a large number of spores, the spore production rate is increased, and the spore production quantity is more than 5 times of the non-induced spore production quantity. Meanwhile, the biocontrol and growth promoting capabilities of the prepared pythium oligandrum spores are not reduced.

The invention discloses a disease-pest-resistant angelica sinensis seedling culture substrate, and a preparation method thereof. The substrate comprises the following forming components in a weight proportion: 25-30 parts of a fermentation material, 20-40 parts of turf, 20-40 parts of coconut residuum, 4-6 parts of a humic acid, 4-6 parts of bone meal, and 1-2 parts of a compound microbial agent,wherein the fermentation material is prepared by mixing and fermenting cow dung, saw dust and corn straw; the bone meal is one of yak bone meal, beef cattle bone meal, and dairy cattle bone meal or multiple combinations in arbitrary proportions; and the compound microbial agent is prepared by a bacteria vector and a microbial agent, and the microbial agent is formed by one of pythium oligandrum, bacillus polymyxa, trichoderma harzianum and bacillus subtilis or multiple combinations in arbitrary proportions. The angelica sinensis seedling culture substrate is easy to obtain raw materials, low in cost, and easy to operate in a preparation process of the substrate. Compared with a current angelica sinensis culture substrate commodity available in the market, a survival rate and a yield of angelica sinensis seedlings cultured by using the substrate are improved, plant diseases and insect pests of angelica sinensis are remarkably reduced, and pesticide application is reduced.

The invention belongs to the technical field of prevention and treatment on fruit plant diseases, and particularly relates to a prevention and treatment method for plum tree gummosis. The prevention and treatment method is characterized by comprising a prevention method for gummosis and a treatment method for gummosis. The prevention and treatment method disclosed by the invention adopts pythium oligandrum to prevent and treat gummosis, is high in cure rate and low in recurrence rate, and has good effect on a plum tree orchard.

The invention discloses application of pythium oligandrum fermentation liquor as a preservative for oranges and tangerines. The application method comprises the following steps: preparing pythium oligandrum fermentation liquor; conducting centrifugation with a speed of 4000 rounds/min for five minutes; adding 2.5%(W/V) edible solid polyethylene glycol in supernatant; adding water according to a volume ratio of fermentation liquor and water as 1:0-2; stirring the fermentation liquor and the water uniformly; soaking oranges and tangerines for 0.5-1 minute; fishing out the oranges and tangerines, and drying the oranges and tangerines at sun shading and ventilating place; packing the oranges and tangerines in baskets (bags); storing the oranges and tangerines in a natural ventilation storehouse, a cellar chamber or a refrigerating chamber. According to the invention, secondary metabolite can be produced conveniently, economically and on a large scale; the defect that living microbial agents are interfered by human and environmental factors is overcome; the application method is safe, nontoxic, environmental-friendly, convenient to operate, and good in fresh-keeping effect; compared with natural fresh-keeping, the rotting rate of the oranges and tangerines is reduced by more than 80%.

The invention discloses a process for producing a composite biological bacterial fertilizer from livestock and poultry manure, which comprises a composite biological bacterial fertilizer process, and the composite biological bacterial fertilizer process is divided into four sections which are first-stage fermentation treatment, second-stage fermentation treatment, third-stage fermentation treatment and fourth-stage fermentation treatment in sequence. A four-stage fermentation technology of different biological beneficial bacteria is adopted, pollution of ammonia gas to the environment is prevented, the lignin is added, the adsorption effect of the lignin on the heavy metal is fully utilized, damage of the heavy metal to soil and crops is avoided, the comprehensive effect of the fertilizer efficiency and the biological effect is achieved, and according to the characteristics and characteristics of existing biological beneficial bacteria, the fertilizer efficiency is improved. Aerobic fermentation and anaerobic fermentation are combined, and through high-temperature fermentation, high-temperature treatment, in-pile culture of an insecticidal fungicide and culture of soil pathogenic bacteria pythium oligandrum, harmless treatment of the livestock and poultry manure is achieved, and damage to crops and soil caused by application of the livestock and poultry manure which is directly or simply treated into the soil is comprehensively avoided.