5 results about "Pythium oligandrum" patented technology

The invention relates to the field of bactericides, and particularly discloses a microbial bactericide and a preparation method thereof. The microbial bactericide is prepared from the following raw material components: 40 to 45 parts of microbial source fermentation liquor; 50 to 60 parts of a carrier; 2-3 parts of a dispersing aid; 3-5 parts of a surfactant; 0.5 to 1 part of a synergist; microbial sources in the microbial source fermentation liquor comprise bacillus, trichoderma harzianum, trichoderma viride and pythium oligandrum according to the weight ratio of 50: (20-25): (10-15): 10, and the carrier comprises at least two of sodium lactate, calcium sulfate and manganese sulfate; the preparation method comprises the following steps: I, activating and culturing a microbial source; iI, preparing microbial source fermentation liquor; and III, preparing the microbial bactericide. The composition disclosed by the invention has high bactericidal ability, high stability and long-acting bactericidal ability, the prevention and treatment effect can be not lower than 71.5%, and the suspension rate is not lower than 97.85%.

The invention relates to the technical field of hemp plant disease and insect pest control, in particular to a hemp plant disease and insect pest biological control fungicide and a preparation method thereof. The invention provides a biological control fungicide for plant diseases and insect pests of cannabis sativa plants. The microbial agent is prepared from the following components in parts by mass: 10 to 15 parts of bifidobacterium bifidum, 12 to 16 parts of paenibacillus polymyxa, 16 to 20 parts of bacillus amyloliquefaciens, 12 to 16 parts of burkholderia, 13 to 17 parts of pythium oligandrum, 6 to 10 parts of lactobacillus plantarum, 15 to 19 parts of bacillus velezensis, 15 to 19 parts of pseudomonas aeruginosa and 12 to 16 parts of metarhizium anisopliae. The biological control fungicide for the plant diseases and insect pests of the cannabis sativa has a good control effect on various plant diseases and insect pests of the cannabis sativa, has a remarkable growth promoting effect on the cannabis sativa when being used as a cannabis sativa seed dressing agent, and has a good application prospect.

A Pythium oligandrum strain EMF915 is preserved in China Center for Type Culture Collection of Wuhan University on May 24, 2022, with a preservation number of CCTCC NO: M 2022710 and an address of Wuhan University, Hubei Province, China, and a nucleotide sequence as shown in the sequence table SEQ ID NO: 1. The strain is used for preparing a Prunus domestica, Fragaria ananassa, Prunus cerasus, Actinidia deliciosa biological preservative. Compared with general storage, the biological preservative treatment reduces the rot rate of Prunus domestica by 65-75%, reduces the rot rate of Fragaria ananassa by 20-30%, reduces the rot rate of Prunus cerasus by 40-50%, reduces the rot rate of Actinidia deliciosa by 50-60%, and reduces the rot rate of Lycopersicon lycopersicum by 80-90%. The biological preservative prepared by the Pythium oligandrum strain EMF915 can be used for postharvest preservation of fruits and vegetables and prolongs the shelf life.

This invention discloses a broad-spectrum Pythium oligandrum strain, Pyo-P2, with broad-spectrum growth-promoting and disease-resistant functions, and its applications. The preservation number of this Pythium oligandrum strain, Pyo-P2, is CGMCC No. 42579. This strain can significantly promote the plant growth and increase biomass of various crops such as potatoes, rice, and soybeans. Simultaneously, it exhibits significant inhibitory activity against multiple pathogens, including Phytophthora soybeanae, Phytophthora pathogenica, Rhizoctonia solani, and Fusarium graminearum. This strain and its microbial preparations possess both growth-promoting and disease-preventing functions, are environmentally friendly, and have broad application prospects in agriculture.

The application belongs to the technical field of genetic engineering, and specifically discloses a gene PoRWP2 for improving the production of Pythium oligandrum oospores and an application thereof. The application analyzes and transforms the Pythium oligandrum PoRWP2 gene, which is composed of 837 nucleotides, and the protein coded by the gene contains 278 amino acids, and can regulate the sexual development and oospore formation process of Pythium oligandrum. The application verifies the function of the PoRWP2 gene, provides a new direction for regulating the physiological function of Pythium oligandrum by using the gene and improving the sporulation capacity of the biocontrol bacteria, and provides a theoretical basis and technical support for developing biocontrol agents based on Pythium oligandrum and carrying out biological control of oomycete diseases.