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Pythium oligandrum spore production inducer as well as preparation method and application thereof

A technology of Pythium oligandrum and inducer, which is applied in the field of life sciences, can solve the problems that it is difficult to meet mass production, and the number and efficiency of Pythium oligandrum spore suspension need to be improved, so as to improve the sporulation rate and save precious time , good economic and social benefits

Pending Publication Date: 2022-07-29
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The process of biopesticide processing usually involves the preparation of fungal spores. The spores of Pythium oligandrum are generally directly placed into the liquid culture medium to obtain the mycelium, and then the spores are obtained by whipping or shaking. The number and efficiency of the spore suspension of Pythium oligandrum spores obtained by this method need to be improved, and it is difficult to meet the needs of mass production

Method used

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  • Pythium oligandrum spore production inducer as well as preparation method and application thereof
  • Pythium oligandrum spore production inducer as well as preparation method and application thereof
  • Pythium oligandrum spore production inducer as well as preparation method and application thereof

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preparation example Construction

[0020] On the other hand, the present application provides a preparation method of any of the aforementioned Pythium oligandrum sporulation inducers, comprising the following steps:

[0021] Weigh 1-50 g of sodium molybdate, 1-100 g of ammonium sulfate, 1-100 g of sodium iron EDTA, make up to 1000 ml with water, mix well and sterilize to obtain a sporulation inducer.

[0022] In another aspect, the present application provides a medium for inducing sporulation of Pythium oligandrum, comprising a basal medium and any one of the aforementioned Pythium oligandrum sporulation inducers.

[0023] In some embodiments, in the sporulation medium for inducing Pythium oligandrum, the final concentration of any of the aforementioned Pythium oligandrum sporulation inducers is 0.01-10%; preferably 0.1-5%; more preferably 0.1 -2%.

[0024] In some embodiments, the basal medium is V8 medium.

[0025] In some embodiments, the V8 medium comprises V8 vegetable juice, calcium carbonate and wate...

Embodiment 1

[0043] Example 1: Preparation of inducing sporulation medium

[0044] (1) Take an appropriate amount of V8 vegetable juice (American Campbell, item number: Y6GpRQmQ), centrifuge at 4000 r / min for 10 min, take 100 ml of supernatant, add 1 g of calcium carbonate to it, mix and dissolve, and use supernatant to dissolve the obtained V8 vegetable juice calcium carbonate solution. The volume of pure water was adjusted to 1000 mL, and it was sterilized at 121° C. for 20 min to obtain V8 medium.

[0045] (2) Weigh 1 g of sodium molybdate, 1 g of ammonium sulfate, and 10 g of sodium iron EDTA, make up to 1000 mL with ultrapure water, and sterilize at 121° C. for 20 min after mixing to obtain a Pythium oligandrum sporulation inducer.

[0046] (3) When using, add Pythium oligandrum sporulation inducer to the V8 medium to make the final concentration of the Pythium oligandrum sporulation inducer to be 0.1%, and mix well for use.

Embodiment 2

[0047] Example 2: Preparation of inducing sporulation medium

[0048] (1) Take an appropriate amount of V8 vegetable juice, centrifuge at 4000 r / min for 10 min, take 100 ml of supernatant, add 1 g of calcium acid to it, mix and dissolve, and dilute the obtained V8 vegetable juice calcium carbonate solution to 1000 mL with ultrapure water. It was sterilized at 121°C for 20 minutes.

[0049](2) Weigh 5 g of sodium molybdate, 5 g of ammonium sulfate, and 50 g of sodium iron EDTA, make up to 1000 mL with ultrapure water, and sterilize at 121° C. for 20 minutes after mixing to obtain a Pythium oligandrum sporulation inducer.

[0050] (3) When in use, add Pythium oligandrum sporulation inducer to the V8 medium to make the final concentration of the Pythium oligandrum sporulation inducer to be 5%, and mix well for use.

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Abstract

The invention relates to a pythium oligandrum spore production inducer, a pythium oligandrum spore production inducing medium containing the inducer, and a preparation method and application of the pythium oligandrum spore production inducing medium. By adding the spore production inducer, the pythium oligandrum mycelium can quickly produce a large number of spores, the spore production rate is increased, and the spore production quantity is more than 5 times of the non-induced spore production quantity. Meanwhile, the biocontrol and growth promoting capabilities of the prepared pythium oligandrum spores are not reduced.

Description

technical field [0001] The invention relates to the technical field of life sciences, in particular to a sporulation inducer of Pythium oligandrum, a preparation method and an application thereof. Background technique [0002] Pythium oligandrum, belonging to the phylum Oomycetes, is a powerful aggressive parasitic microorganism that widely exists in soil and plant rhizosphere, and has broad-spectrum resistance to pathogenic microorganisms. Pythium oligandrum colonizes the rhizosphere of crops and can promote plant rooting and growth. It is a microorganism with both disease prevention and growth promotion functions. The spores of Pythium oligandrum are the main components that can inhibit the infection of pathogenic bacteria and promote the growth of plants. The currently commercialized product "Dolly Life" is a product jointly developed by ImageTrade International Co., Ltd. and Czech Biologics Co., Ltd. The microbial preparation for broad-spectrum sterilization of the acti...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/14C12N3/00C12R1/645
CPCC12N1/38C12N1/14C12N3/00
Inventor 张雷刚王子洋熊雨洁生弘杰余向阳
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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