Seedling raising method for Angelica sinensis by using microbial agent, and prevention and treatment method for soil-borne diseases
A microbial agent and self-made microorganism technology, which is applied in the application field of agricultural microbial ecological technology, can solve the problems of environmental pollution, drug residue exceeding the standard, etc., and achieve the effect of improving quality and reducing dosage and residue
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Embodiment 1
[0028] 1. Selection of Angelica Seedling Land
[0029] The selection of the Angelica seedling-raising land of the present invention: the shady slope habitat of Wagougou, Minxian County, altitude: 2570 meters. The area of the experimental plot is 1m×5m=5m 2 , with three replicates per treatment.
[0030] 2. Seed selection
[0031] The present invention uses naturally air-dried, worm-free, and mildew-free Angelica sinensis seeds as the provenance, the thousand-grain weight of the seeds is 2.0 g, the normal germination rate is 30%-35%, and the conventional seedling density is 200 plants / m 2 According to this, the dosage of angelica seeds is calculated as 6g / m 2 . Therefore, the amount of seeds used in each experimental plot is 30g.
[0032] 3. Microbial agent seed dressing
[0033] PGPR bacterial agent treatment: Weigh 90g of Angelica sinensis seeds into a sterile container, add 9ml of effective viable bacteria to 10 10 The laboratory made PGPR bacterial agent, and then ...
Embodiment 2
[0051] 1. Selection of Angelica Seedling Land
[0052]The selection of the Angelica seedling-raising land of the present invention: the shady slope habitat of Wagougou, Minxian County, altitude: 2570 meters. The area of the experimental plot is 1m×5m=5m 2 , with three replicates per treatment.
[0053] 2. Seed selection
[0054] The present invention uses naturally air-dried, worm-free, and mildew-free Angelica sinensis seeds as the provenance, the thousand-grain weight of the seeds is 2.0 g, the normal germination rate is 30%-35%, and the conventional seedling density is 200 plants / m 2 According to this, the dosage of angelica seeds is calculated as 6g / m 2 . Therefore, the amount of seeds used in each experimental plot is 30g.
[0055] 3. Microbial agent seed dressing
[0056] AMF inoculant treatment: Weigh 90g of Angelica sinensis seeds into a sterile container, add 90g of self-made AMF inoculum containing mycelia inside and outside the root, infected root segments a...
Embodiment 3
[0074] 1. Selection of Angelica Seedling Land
[0075] The selection of the Angelica seedling-raising land of the present invention: the shady slope habitat of Wagougou, Minxian County, altitude: 2570 meters. The area of the experimental plot is 1m×5m=5m 2 , with three replicates per treatment.
[0076] 2. Seed selection
[0077] The present invention uses naturally air-dried, worm-free, and mildew-free Angelica sinensis seeds as the provenance, the thousand-grain weight of the seeds is 2.0 g, the normal germination rate is 30%-35%, and the conventional seedling density is 200 plants / m 2 According to this, the dosage of angelica seeds is calculated as 6g / m 2 . Therefore, the amount of seeds used in each experimental plot is 30g.
[0078] 3. Microbial agent seed dressing
[0079] PO+PB compound fungal agent treatment: Weigh 90g Heduo angelica seeds into a sterile container, add 10 6 4.5g of PO+PB composite fungal agent per spore / g, and then add 90ml of sterile water to...
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