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Seedling raising method for Angelica sinensis by using microbial agent, and prevention and treatment method for soil-borne diseases

A microbial agent and self-made microorganism technology, which is applied in the application field of agricultural microbial ecological technology, can solve the problems of environmental pollution, drug residue exceeding the standard, etc., and achieve the effect of improving quality and reducing dosage and residue

Active Publication Date: 2016-10-12
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Another object of the present invention is to provide a new method for the prevention and treatment of soil-borne diseases of Angelica sinensis through the approach of growing seedlings with microbial agents, so as to solve the problems of environmental pollution and drug residues exceeding the standard caused by excessive use of chemical pesticides

Method used

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  • Seedling raising method for Angelica sinensis by using microbial agent, and prevention and treatment method for soil-borne diseases
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  • Seedling raising method for Angelica sinensis by using microbial agent, and prevention and treatment method for soil-borne diseases

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Experimental program
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Effect test

Embodiment 1

[0028] 1. Selection of Angelica Seedling Land

[0029] The selection of the Angelica seedling-raising land of the present invention: the shady slope habitat of Wagougou, Minxian County, altitude: 2570 meters. The area of ​​the experimental plot is 1m×5m=5m 2 , with three replicates per treatment.

[0030] 2. Seed selection

[0031] The present invention uses naturally air-dried, worm-free, and mildew-free Angelica sinensis seeds as the provenance, the thousand-grain weight of the seeds is 2.0 g, the normal germination rate is 30%-35%, and the conventional seedling density is 200 plants / m 2 According to this, the dosage of angelica seeds is calculated as 6g / m 2 . Therefore, the amount of seeds used in each experimental plot is 30g.

[0032] 3. Microbial agent seed dressing

[0033] PGPR bacterial agent treatment: Weigh 90g of Angelica sinensis seeds into a sterile container, add 9ml of effective viable bacteria to 10 10 The laboratory made PGPR bacterial agent, and then ...

Embodiment 2

[0051] 1. Selection of Angelica Seedling Land

[0052]The selection of the Angelica seedling-raising land of the present invention: the shady slope habitat of Wagougou, Minxian County, altitude: 2570 meters. The area of ​​the experimental plot is 1m×5m=5m 2 , with three replicates per treatment.

[0053] 2. Seed selection

[0054] The present invention uses naturally air-dried, worm-free, and mildew-free Angelica sinensis seeds as the provenance, the thousand-grain weight of the seeds is 2.0 g, the normal germination rate is 30%-35%, and the conventional seedling density is 200 plants / m 2 According to this, the dosage of angelica seeds is calculated as 6g / m 2 . Therefore, the amount of seeds used in each experimental plot is 30g.

[0055] 3. Microbial agent seed dressing

[0056] AMF inoculant treatment: Weigh 90g of Angelica sinensis seeds into a sterile container, add 90g of self-made AMF inoculum containing mycelia inside and outside the root, infected root segments a...

Embodiment 3

[0074] 1. Selection of Angelica Seedling Land

[0075] The selection of the Angelica seedling-raising land of the present invention: the shady slope habitat of Wagougou, Minxian County, altitude: 2570 meters. The area of ​​the experimental plot is 1m×5m=5m 2 , with three replicates per treatment.

[0076] 2. Seed selection

[0077] The present invention uses naturally air-dried, worm-free, and mildew-free Angelica sinensis seeds as the provenance, the thousand-grain weight of the seeds is 2.0 g, the normal germination rate is 30%-35%, and the conventional seedling density is 200 plants / m 2 According to this, the dosage of angelica seeds is calculated as 6g / m 2 . Therefore, the amount of seeds used in each experimental plot is 30g.

[0078] 3. Microbial agent seed dressing

[0079] PO+PB compound fungal agent treatment: Weigh 90g Heduo angelica seeds into a sterile container, add 10 6 4.5g of PO+PB composite fungal agent per spore / g, and then add 90ml of sterile water to...

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Abstract

The invention discloses an application of a microbial agent in seedling raising of Angelica sinensis, and a prevention and treatment method for soil-borne diseases. The microbial agent provided by the invention comprises three self-prepared microbial agents and a commercial microbial agent which are respectively used for seed dressing of Angelica sinensis and seedling raising in an uncultivated land; and through counting of the weight of an Angelica sinensis seedling, the number of Angelica sinensis plants and the level of Angelica sinensis seedlings in a test plot of Angelica sinensis seedlings, feasibility of the application the microbial agent in seedling raising of Angelica sinensis and prevention and treatment effects on the soil-borne diseases are researched. A composite fungal agent (Pythium oligandrum and Beauveria bassiana, PO+BB) provided by the invention has best effects on seedling raising of Angelica sinensis and the prevention and treatment of the soil-borne diseases, and is superior to the commercial fungal agent Polyversum Pythium oligandrum oospores; and the composite fungal agent has good application prospects in agriculture and production of traditional Chinese medicinal materials.

Description

technical field [0001] The invention relates to a method for raising seedlings with microbial agent of angelica and a method for preventing and controlling soil-borne diseases, and belongs to the application field of agricultural microorganism ecological technology. Background technique [0002] Angelica [ Angelica sinensis (Oliv.) Diels], also known as Mingui, Qingui, Xianggui, Chuangui, etc., is a perennial herb of Umbelliferae and a well-known authentic Chinese medicinal material. Angelica is suitable for planting in the alpine and damp areas at an altitude of 2000-2800m. Gansu is the main producing area of ​​angelica in my country, and its annual cultivation area and total output account for more than 90% of the country. Due to the contradiction between the limited suitable living area and the annual demand of the market, the phenomenon of continuous cropping of angelica is common, resulting in serious soil-borne damage such as root rot of angelica root rot, resulting i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01C1/06A01G13/00A01N63/00A01P1/00A01P3/00
CPCA01C1/06A01G13/00A01G22/00A01N63/00
Inventor 祝英杨晖王治业彭轶楠巩晓芳郭增祥周剑平张军季彬
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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