Biological repairing agent of soil

A soil biology and remediation agent technology, applied in the field of agricultural microorganisms, can solve the problems of soil microbial imbalance, backward fertilization technology, and unfavorable crop absorption and utilization.

Inactive Publication Date: 2017-06-13
三门峡龙飞生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There are two main technical bottlenecks restricting the industry of soil improvement and restoration technology products. One is the destruction of the agricultural ecological environment, the imbalance of microorganisms in some soils, and the decline in self-healing ability. The application technology of soil improvement and restoration of beneficial microorganisms is far from satisfactory due to The adverse effects caused by the destruction of soil micro-ecology, and most of the microbial application technologies for soil improvement and restoration are still in th

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0133] Embodiment 1: preparation of soil bioremediation agent of the present invention

[0134] This embodiment implements as follows:

[0135] First, prepare the fermentation product of Sphingobacterium multivorum

[0136] A. Activation culture

[0137] Prepare beef extract peptone liquid medium: add 5g beef extract, 10g peptone, 10g glucose, and 5g NaCl to 1000ml distilled water, boil to dissolve, and then sterilize at 121°C for 30min to obtain beef extract peptone liquid medium;

[0138] Under sterile conditions, inoculate 0.1 g of Sphingobacterium multivorum into 200 mL of the above-mentioned beef extract-peptone liquid medium, and culture it for 48 hours at a temperature of 30°C and a rotation speed of 180 r / min to obtain Sphingobacterium multivorum activated cultures;

[0139] B. Strain purification

[0140] Add 5.0g beef extract, 10.0g peptone, 5.0g sodium chloride and 18-20g nutrient agar to 1000ml water, dissolve, and then sterilize at 121°C for 30min to obtain be...

Embodiment 2

[0153] Embodiment 2: preparation of soil bioremediation agent of the present invention

[0154] This embodiment implements as follows:

[0155] First, prepare the fermentation product of Sphingobacterium multivorum

[0156] A. Activation culture

[0157] Beef extract peptone broth was prepared in the same manner as in Example 1.

[0158] Under sterile conditions, inoculate 0.1 g of Sphingobacterium multivorum into 200 mL of the above-mentioned beef extract-peptone liquid medium, and culture it for 48 hours at a temperature of 30°C and a rotation speed of 180 r / min to obtain Sphingobacterium multivorum activated cultures;

[0159] B. Strain purification

[0160] Beef extract peptone solid medium was prepared in the same manner as in Example 1, and strains were purified.

[0161] C. Seed liquid culture

[0162] A seed liquid medium was prepared in the same manner as in Example 1.

[0163] Pick a single Sphingobacterium multivorum colony from the beef extract peptone mediu...

Embodiment 3

[0171] Embodiment 3: preparation of soil bioremediation agent of the present invention

[0172] This embodiment implements as follows:

[0173] First, prepare Sphingobacterium multivorum

[0174] A. Activation culture

[0175] Beef extract peptone broth was prepared in the same manner as in Example 1.

[0176] Under sterile conditions, inoculate 0.1g of Sphingobacterium multivorum provided by Shanghai Fengshou Biotechnology Co., Ltd. into 200mL of the above-mentioned beef extract peptone liquid medium, under the conditions of temperature 30°C and rotation speed 180r / min Cultivate for 48 hours to obtain an activated culture of Sphingobacterium multivorum;

[0177] B. Strain purification

[0178] The preparation of beef extract peptone solid medium was carried out in the same manner as in Example 1, and strain purification was carried out.

[0179] C. Seed liquid culture

[0180] A seed liquid medium was prepared in the same manner as in Example 1.

[0181] Pick a single ...

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Abstract

The invention relates to a biological repairing agent of soil. The biological repairing agent is prepared from bacillus cereus bacterium powder, pythium oligandrum bacterium powder, trichoderma viride bacterium powder, a sphingobacterium multivorum fermented product, mushroom dreg of edible mushrooms, calcium amino acid chelate, zinc amino acid chelate and DA-6; the biological repairing agent can be used for degrading pollution of petroleum agriculture and pesticide residues, and can be applied to a farmland so that the aims of ameliorating soil and preventing diseases, reducing the residues and promoting growth so as to improve the micro-ecological activity and self-repairing capability of the soil, increase the yield of crops, improve the quality and maintain the agricultural ecological health can be realized.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of agricultural microorganisms. More specifically, the present invention relates to a soil bioremediation agent. [0003] 【Background technique】 [0004] Soil is an important carrier and place for material transformation and energy exchange in agricultural production, and it is also the core part of the ecosystem. Among various environmental elements, soil is the final receptor of pollutants. With the widespread application of petroleum products and chemical pesticides on farmland, petroleum pollutants and pesticide residues left in the soil have become an urgent problem to be solved because they are difficult to eradicate. [0005] A series of outstanding problems caused by petroleum agriculture, such as the deterioration of the ecological environment, soil salinization and hardening, the proliferation of diseases and insect pests, serious white pollution, and the decline in the quality of agricult...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/04C12N1/20C12N1/14C12R1/085C12R1/645C12R1/885C12R1/01C05G3/80
CPCC05C11/00C05G3/00C05G3/80C12N1/14C12N1/20C05F11/08
Inventor 赵跃进赵跃文赵跃民易卿谢松张盼赵洁赵科
Owner 三门峡龙飞生物工程有限公司
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