Biological repairing agent of soil
A soil biology and remediation agent technology, applied in the field of agricultural microorganisms, can solve the problems of soil microbial imbalance, backward fertilization technology, and unfavorable crop absorption and utilization.
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Embodiment 1
[0133] Embodiment 1: preparation of soil bioremediation agent of the present invention
[0134] This embodiment implements as follows:
[0135] First, prepare the fermentation product of Sphingobacterium multivorum
[0136] A. Activation culture
[0137] Prepare beef extract peptone liquid medium: add 5g beef extract, 10g peptone, 10g glucose, and 5g NaCl to 1000ml distilled water, boil to dissolve, and then sterilize at 121°C for 30min to obtain beef extract peptone liquid medium;
[0138] Under sterile conditions, inoculate 0.1 g of Sphingobacterium multivorum into 200 mL of the above-mentioned beef extract-peptone liquid medium, and culture it for 48 hours at a temperature of 30°C and a rotation speed of 180 r / min to obtain Sphingobacterium multivorum activated cultures;
[0139] B. Strain purification
[0140] Add 5.0g beef extract, 10.0g peptone, 5.0g sodium chloride and 18-20g nutrient agar to 1000ml water, dissolve, and then sterilize at 121°C for 30min to obtain be...
Embodiment 2
[0153] Embodiment 2: preparation of soil bioremediation agent of the present invention
[0154] This embodiment implements as follows:
[0155] First, prepare the fermentation product of Sphingobacterium multivorum
[0156] A. Activation culture
[0157] Beef extract peptone broth was prepared in the same manner as in Example 1.
[0158] Under sterile conditions, inoculate 0.1 g of Sphingobacterium multivorum into 200 mL of the above-mentioned beef extract-peptone liquid medium, and culture it for 48 hours at a temperature of 30°C and a rotation speed of 180 r / min to obtain Sphingobacterium multivorum activated cultures;
[0159] B. Strain purification
[0160] Beef extract peptone solid medium was prepared in the same manner as in Example 1, and strains were purified.
[0161] C. Seed liquid culture
[0162] A seed liquid medium was prepared in the same manner as in Example 1.
[0163] Pick a single Sphingobacterium multivorum colony from the beef extract peptone mediu...
Embodiment 3
[0171] Embodiment 3: preparation of soil bioremediation agent of the present invention
[0172] This embodiment implements as follows:
[0173] First, prepare Sphingobacterium multivorum
[0174] A. Activation culture
[0175] Beef extract peptone broth was prepared in the same manner as in Example 1.
[0176] Under sterile conditions, inoculate 0.1g of Sphingobacterium multivorum provided by Shanghai Fengshou Biotechnology Co., Ltd. into 200mL of the above-mentioned beef extract peptone liquid medium, under the conditions of temperature 30°C and rotation speed 180r / min Cultivate for 48 hours to obtain an activated culture of Sphingobacterium multivorum;
[0177] B. Strain purification
[0178] The preparation of beef extract peptone solid medium was carried out in the same manner as in Example 1, and strain purification was carried out.
[0179] C. Seed liquid culture
[0180] A seed liquid medium was prepared in the same manner as in Example 1.
[0181] Pick a single ...
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