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Pythium oligandrum graphene material composite biocontrol preparation and preparation method thereof

A graphene-based material, Pythium oligandrum technology, applied in the field of biological control, can solve the problems of polluted soil and water sources, healthy diet and green environment, insignificant effects, hidden dangers of ecological safety, pesticide residues, etc., and meets the requirements of low production equipment, Good promotion and application prospects, low cost effect

Active Publication Date: 2021-06-01
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of the first control method is not significant and there are hidden dangers in ecological security, while drug control cannot meet the requirements of human beings to pursue a healthy diet and a green environment due to the shortcomings of pesticide residues, polluted soil and water sources, etc.

Method used

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  • Pythium oligandrum graphene material composite biocontrol preparation and preparation method thereof
  • Pythium oligandrum graphene material composite biocontrol preparation and preparation method thereof
  • Pythium oligandrum graphene material composite biocontrol preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 Effect of different concentrations of graphene materials on the growth of Pythium oligandrum

[0024] The PDA liquid medium was divided into 100mL Erlenmeyer flasks, 40mL per bottle. Experimental group: Three carbon materials, graphene, graphene oxide and three-dimensional network activated carbon, were added to the liquid medium at a ratio of 5 mg / L, 50 mg / L and 100 mg / L respectively, and shaken well; the control group did not add additional, PDA liquid medium only. Three parallels were set up for each experimental group.

[0025] Put the same amount of Pythium oligandrum agent into the above-mentioned Erlenmeyer flask, and culture it in a constant temperature shaker at 28°C at 150r / min. After 5-7 days, a large amount of flocculent mycelium grew in each bottle. And when the culture medium of the experimental group became clear and translucent, the culture was stopped at the same time.

[0026] The mycelium in each Erlenmeyer flask was taken out, rinsed ...

Embodiment 2

[0027] Example 2 Preparation of Pythium oligandrum Graphene Composite Biocontrol Preparation

[0028] 1. Divide the PDA liquid medium into 100mL Erlenmeyer flasks, 40ml per bottle. Add graphene to the Erlenmeyer flask by 5mg / L, 50 mg / L and 100mg / L, and stir evenly;

[0029] 2. Add Pythium oligandrum to the liquid medium prepared in step 1, and cultivate at 28°C and 150r / min until 5-7 days later, a large number of flocculent mycelia of Pythium oligandrum in dark gray clusters grow, and when the medium is clear and translucent Stop culturing, such as figure 1 shown;

[0030] 3. Take out the mycelium, wash off the culture medium on the mycelia with deionized water, and the resulting Pythium oligandrum graphene composite biocontrol preparation.

Embodiment 3

[0031] Example 3 Preparation of Pythium oligandrum Graphene Oxide Composite Biocontrol Preparation

[0032] 1. Divide the PDA liquid medium into 100mL Erlenmeyer flasks, 40ml per bottle. Add graphene oxide to the Erlenmeyer flask at 5 mg / L, 50 mg / L and 100 mg / L, and stir evenly;

[0033] 2. Add Pythium oligandrum to the liquid medium prepared in step 1, and cultivate at 28°C and 150r / min until 5-7 days later, a large number of flocculent mycelia of Pythium oligandrum in dark gray clusters grow, and when the medium is clear and translucent stop cultivating;

[0034] 3. Take out the mycelium, wash off the culture medium on the mycelia with deionized water, and the resulting Pythium oligandrum graphene oxide composite biocontrol preparation.

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Abstract

The invention discloses a pythium oligandrum graphene material composite biocontrol preparation and a preparation method thereof, the pythium oligandrum graphene material composite biocontrol preparation is prepared by introducing a graphene material into a liquid culture medium, and the biocontrol performance of pythium oligandrum can be effectively improved. The inhibition rate of the biocontrol preparation of the pythium oligandrum composite graphene material provided by the invention on alternaria solani, fusarium oxysporum cucumber specialty and fusarium solani is greatly higher than that of a common pythium oligandrum agent. The preparation method of the pythium oligandrum graphene material composite biocontrol preparation is simple in preparation process, low in requirement for production equipment, low in cost and suitable for large-scale production, and the composite biocontrol preparation is good in biocontrol effect and has good application and popularization prospects.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to a Pythium oligandrum graphene material composite biocontrol preparation and a preparation method thereof. Background technique [0002] When crops are grown on a large scale, they are often threatened by a variety of pathogenic bacteria, such as oomycete pathogens of Pythium and Phytophthora, which reduce yields. Usually, the transmission routes of pathogenic bacteria mainly include wind, diseased seedlings, soil, irrigation or rainwater, etc. When they come into contact with crops with water flow, they will parasitize on the surface and form resting spores, and then infect and enter the host body. Infestation can cause Pythium root rot on seedlings. Under suitable environmental conditions, the outbreak will cause the seedlings to wither gradually in a short period of time, and in severe cases, it may cause failure of the harvest. [0003] At present, the preventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01N59/00A01P3/00C12R1/645
CPCC12N1/14A01N63/30A01N59/00
Inventor 郑群毛婷王沛雅方彦昊季彬王治业杨晖祝英
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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