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Biocontrol strain KMXU22 capable of preventing and treating soft rot of konjak and antibiological inoculant of biocontrol strain KMXU22

A KMXU22, biocontrol strain technology, applied in biocides, plant growth regulators, bacteria, etc., can solve the problems of high chemical pesticides and pesticide residues, difficult control and high cost of konjac soft rot, and improve konjac planting soil. , Control the occurrence of soft rot disease and improve the effect of biocontrol

Inactive Publication Date: 2013-04-03
KUNMING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The object of the present invention is to provide a kind of biocontrol bacterial strain KMXY22 and biocontrol strain KMXY22 for preventing and treating konjac soft rot in order to solve the problems that the control of konjac soft rot is difficult and costly, and chemical pesticides and pesticide residues are not suitable for use in current agricultural production. Bacteria

Method used

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  • Biocontrol strain KMXU22 capable of preventing and treating soft rot of konjak and antibiological inoculant of biocontrol strain KMXU22
  • Biocontrol strain KMXU22 capable of preventing and treating soft rot of konjak and antibiological inoculant of biocontrol strain KMXU22

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of biocontrol agent

[0028] Biocontrol bacterium agent of the present invention obtains through following steps:

[0029] a. Transplant strain KMXY22 into a test tube containing LB medium for one-ring activation, and culture at 32°C for 48 hours; the formula of LB liquid medium is: tryptone (Tryptone) 10g / L, yeast extract (Yeast extract) 5g / L L, sodium chloride (NaCl) 5g / L, pH7.0;

[0030] b. Take a ring of activated bacterial lawn and transfer it into a 600ml 1000ml Erlenmeyer flask. The formula is: tryptone (Tryptone) 10g / L, yeast extract (Yeast extract) 5g / L, sodium chloride (NaCl) 5g / L , in the LB culture medium with pH 7.0, cultured at 120 rpm and 32°C for 72 hours to obtain the fermentation broth;

[0031] c. Inoculate the fermentation broth in the same LB culture broth as in step b in the seed tank at the ratio of inoculum 1:200 for fermentation and culture. The temperature of the seed tank is 32°C, and it is obtained after 18 hou...

Embodiment 2

[0033] Embodiment 2: plate antagonism test

[0034] The inhibitory effect of Konjac soft rot pathogen was evaluated by punching method. Konjac soft rot disease pathogenic bacteria suspension is evenly spread on the beef extract peptone medium (beef extract 3.0g, NaCl 5.0g, peptone 10.0g, water 1000ml, agar 10.0g, pH7.0, the same below), with no Punch four 6mm holes evenly with a bacteria puncher, and add 50 μl of KMXY22 fermentation broth into the holes. After culturing at 32°C for one day, the average diameter of the inhibition zone reached 30.2 mm. It shows that the KMXY22 strain has a significant inhibitory effect on Konjac soft rot.

Embodiment 3

[0035] Embodiment 3: Greenhouse control effect test of konjac soft rot

[0036] Set up three treatments, treatment 1 is the sterile water control group, treatment 2 is the KMXY22 bacterial agent, and treatment 3 is the bactericide 72% agricultural streptomycin sulfate (Qingdao Nongle Chemical Weiye Co., Ltd.) 2000 times solution. Konjac annual bulbs are planted in the soil of Konjac soft rot fungus. Before planting, soak the seeds with KMXY22 bacterial agent for 30 minutes. After 15-20 days of planting, use 1:40 times of KMXY22 bacterial agent for hole irrigation, 8-10 times per kilogram of liquid hole irrigation. crops; 100 days after the crops are planted, use 1:40 times KMXY22 bacterial agent to irrigate the roots once, and irrigate the roots of 5-6 crops per kilogram of liquid. Other clear water controls and 72% agricultural streptomycin sulfate pesticides were treated with the same method and dosage as above. The amount of the preparation applied per 667㎡ is determined a...

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Abstract

The invention relates to a biocontrol strain KMXU22 capable of preventing and treating soft rot of konjak and an antibiological inoculant of the biocontrol strain KMXU22, and belongs to the technical field of biopesticide. The preservation number of the strain KMXU22 provided by the invention is CGMCC NO. 6597. The antibiological inoculant is obtained by the following steps of: transplanting the strain KMXU22 into a test tube, carrying out one-cycle activation, and culturing for 48 hours at the temperature of 32 DEG C, wherein LB liquid culture medium in the test tube contains 10g / L tryptone, 5g / L yeast extract and 5g / L sodium chloride, and the pH value is 7.0; transferring an activated bacterial lawn into 600ml of LB culture solution which is filled in a 1000ml conical flask and has the same formula as a., and culturing for 72 hours under the conditions that the rotating speed is 120r / min and the temperature is 32 DEG C, so that zymogeneous bacteria solution is obtained; inoculating the zymogeneous bacteria in a seed tank in the ratio of 1: 200 by inoculation amount, and fermenting for 18-20 hours at the temperature of 32 DEG C, so that fermentation seed strains are obtained; and inoculating the fermentation seed strains into a fermentation tank in the ratio of 1: 20 by the inoculation amount, fermenting for 72-96 hours at the temperature of 32 DEG C, and storing the fermented seed strains as the antibiological inoculant when strain content is more than 2*10<10>-2*10<11> CFU / ml and the pH value is 7.0-7.6. The antibiological inoculant provided by the invention has the advantages that cost is low, no position or pollution is produced, and biocontrol effect is good.

Description

Technical field: [0001] The invention relates to a biocontrol bacterial strain KMXY22 for preventing and treating konjac soft rot and a biocontrol bacterial agent thereof, belonging to the technical field of biological pesticides. Background technique: [0002] Konjac (Amorphophallus konjac) is a perennial herb and the only special economic crop high in konjac glucomannan (KGM) found so far in the world (Liu Peiying, 2004). Because of its unique physical and chemical properties and functions such as gel compounding, konjac products are currently widely used in food, health care, chemical, pharmaceutical, environmental protection and other fields, and are rated as one of the top ten health foods by WHO. In addition, konjac can also be used as a renewable resource, which can effectively replace other plant gums and relieve resource pressure. The global konjac planting areas are mainly distributed in Asia and Africa, and the artificial large-scale planting is mainly in Japan a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/39
Inventor 余磊赵建荣
Owner KUNMING UNIV
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