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Trichoderma harzianum strain for controlling plant fungus diseases and application thereof

A technology of Trichoderma harzianum and microbial inoculum, which is applied in the field of microorganisms and biological fertilizers, can solve the problems of limited competitive survival ability, single action object, poor effect, etc., and achieves good biological control effect and effect of good control effect.

Inactive Publication Date: 2014-07-09
赵斌 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, most of the Trichoderma fungi known to have control effects on plant fungal diseases are not effective, the target of action is relatively single, they are easily affected by the natural environment, and their ability to compete and survive is limited.

Method used

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  • Trichoderma harzianum strain for controlling plant fungus diseases and application thereof
  • Trichoderma harzianum strain for controlling plant fungus diseases and application thereof
  • Trichoderma harzianum strain for controlling plant fungus diseases and application thereof

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Experimental program
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Effect test

Embodiment 1

[0031] The collection of embodiment 1 soil sample and the separation of Trichoderma harzianum strain of the present invention

[0032] 1. Sample collection

[0033] The samples were collected from the rhizosphere soil of cucumber field in Raoyang, Hebei.

[0034] Remove the 3-5cm topsoil layer in the field with soil-borrowing equipment, dig out the soil together with the roots of the plants, pack them in polyethylene plastic bags, and bring them back to the laboratory for separation.

[0035] 2. Isolation and acquisition of Trichoderma harzianum strain MSB1211

[0036] Air-dry the root system adhering to the soil, pat the root system lightly to make the soil adhering to it fall off, dilute it with sterile water gradient, draw 0.1mL of the diluted solution and drip it into the TSM medium plate base (MgSO 4 ,·7H 2 O 0.2 g, KH 2 PO 4 3H 2 O 0.9 g, KCI 0.25 g, NH 4 NO 3 1.0 g of glucose, 3.0 g of glucose, 0.15 g of rose bengal, 0.25 g of chloramphenicol, 18.0 g of agar, ...

Embodiment 2

[0038] Embodiment 2 Identification of Trichoderma harzianum strain of the present invention

[0039] 1 Morphological identification

[0040] According to the "Fungus Identification Manual", "Trichoderma Classification and Identification" and other methods, use PDA medium to cultivate fungi, observe the growth characteristics of the colony with the naked eye, measure the growth rate of the colony, and observe the shape of the hyphae, whether there is a diaphragm, and the size of the spores under an optical microscope. , shape, type, etc. Through observation, it was found that the mycelium block of 5 mm MSB1211 strain was inoculated on PDA medium, and after 24 hours of constant temperature cultivation at 28 ° C, clear mycelium could be seen with the naked eye, and the colony expanded rapidly. Thick, dense and fluffy, the colony is white at the beginning, and turns green at the later stage due to the production of spores. The back of the colony is colorless at first, and turn...

Embodiment 3

[0045] Embodiment 3 The antibacterial effect of Trichoderma harzianum strain of the present invention

[0046] Experimental group: Trichoderma harzianum MSB1211 bacteria cake and pathogenic bacteria cake were inoculated on the same PDA plate for confrontation culture (the vertical distance between the centers of the two bacteria cakes was 7cm); the PDA plate only inoculated with pathogenic bacteria cake was used as the control group; 25°C After cultivating in the dark for a certain period of time, measure the colony diameter of the pathogen (the average value of the longest diameter and the shortest diameter) and calculate the inhibition rate as shown in Table 2. figure 1 As shown, the antibacterial effect on Fusarium oxysporum of melon is as follows figure 2 As shown, the antibacterial effect on tomato cinerea image 3 shown. It can be seen from the figure that Trichoderma harzianum MSB1211 of the present invention inhibits its growth and continues to grow and parasitiz...

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Abstract

The invention relates to a high-efficiency Trichoderma harzianum for preventing and controlling plant fungal diseases and promoting plant growth. The Trichoderma harzianum described in the present invention is preserved in the General Microorganism Center of China Microorganism Culture Preservation Management Committee, and the preservation number is CGMCC No.7230. The Trichoderma harzianum involved in the present invention has a higher ability to inhibit the growth of phytopathogenic fungi, does not pollute the environment, and is ecologically safe, and can be widely applied to phytopathogenic fungi, especially Pythium spp. (Pythium spp.), Fusarium spp. .), the control of Botrytis cinerea, is a strain with high application value in the biological control of fruits, vegetables and crops. The high-efficiency Trichoderma harzianum is used alone or together with other organic and inorganic nutrients required for plant growth, and the prepared biological compound fertilizer has significant effects on promoting growth, anti-aging, preventing diseases, increasing yield and improving product quality. .

Description

technical field [0001] The invention relates to the field of microorganisms and biological fertilizers, in particular to Trichoderma harzianum, specifically, the invention relates to a Trichoderma harzianum for preventing and controlling plant fungal diseases and an application thereof. Background technique [0002] Plant diseases are one of the main factors affecting agricultural production, and chemical pesticides have always played a very important role in the prevention and control of plant diseases and insect pests. However, due to the extensive use of chemical pesticides, it has brought many negative effects, such as polluting the ecological environment, endangering humans and animals, producing drug resistance, harming non-target organisms, and pesticide residues, which have seriously affected a series of problems such as human health and ecological balance. It prompts people to explore new plant pest control strategies that are harmless to humans and the environment ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01P3/00A01P21/00C05G3/00C12R1/885
Inventor 赵斌董金皋赵巍
Owner 赵斌
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