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Preparation method and application of Pythium oligandrum microbial fertilizer

A technology of Pythium oligandrum and Pythium oligandrum, which is applied in the field of preparation of Pythium oligandrum fertilizer, can solve problems such as poor effect, poor stability, and single function, and achieve no price pressure, good stability, and overcome single function Effect

Inactive Publication Date: 2017-02-22
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is: for Pythium oligandrum oospore preparations in the prior art, it is difficult to germinate and grow mycelia in the field soil, the stability is poor, and the spores in the oospore preparations The number is small, and it cannot effectively compete with microorganisms in the soil, thereby promoting plant growth and inhibiting the technical problems of microorganisms, and providing a Pythium oligandrum rich in oospores and hyphae and low in preparation cost. The preparation method and application of fertilizer overcome the defects of poor stability, poor effect and single function of existing bacterial fertilizer preparations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] One, a kind of preparation method of Pythium oligandrum fertilizer, comprises the steps:

[0022] (1) Solid multiplication strains:

[0023] 1) Use self-isolated Pythium oligandrum strains for solid propagation: use tap water to prepare a solid medium (pH6.3), so that the prepared medium contains 2.0g / L (NH 4 ) 2 SO 4 , 1.0g / L KH 2 PO 4 , 1.0g / L MgSO 4 ,0.5g / LCaCl 2 ,0.5g / L Asparagine, 20.0g / L Glucose, 0.2g / L Vitamin B 1 , 2mL / L trace element mixed solution [every 1L aqueous solution contains 5.0mg MnSO 4 ,5.0mg FeSO 4 ,3.0mg H 3 BO 3 ,2.0mg ZnCl 2 , 0.3mgCuSO 4 ,0.25mg (NH 4 ) 2 MoO 4 ], 20g / L agar.

[0024] 2) Sterilize the prepared solid culture based on steam at 121°C and 1.5 atmospheric pressure for 30 minutes, cool to 45°C, take a 5cm diameter petri dish, pour 5mL of sterilized medium into it, and cool to room temperature. The Pythium oligandrum CQ2010 strain preserved in the petri dish was taken, the hyphae were picked along the edge of the colon...

Embodiment 2

[0034] One, a kind of preparation method of Pythium oligandrum fertilizer, comprises the steps:

[0035] (1) Use the oospore preparation of "Doliweisheng · Pythium oligandrum" for solid propagation:

[0036] 1) Obtain Pythium oligandrum species by oospore preparation:

[0037] Medium selects the improved Martin's agar medium of pH 5.5 for use, and solvent is water and comprises the solute component of following concentration: 20g / L agar, 1.5g / L (NH 4 ) 2 SO 4 , 0.5g / LKH 2 PO 4 , 0.5g / L MgSO 4 , 0.25g / L CaCl 2 , 0.25g / L asparagine, 10.0g / L glucose, 0.1g / L vitamin B 1 , 1mL / L trace element mixed solution; the solvent of described trace element mixed solution is water, and comprises the solute component of following concentration: 2.5mg / LMnSO 4 , 2.5mg / LFeSO 4 , 1.5mg / L H 3 BO 3 , 1.0mg / LZnCl 2 , 0.15mg / L CuSO 4 and 0.15mg / L (NH 4 ) 2 MoO 4 , dissolve at 100°C and shake well, take 5mL culture base into 25mL test tube, steam sterilize at 121°C for 30 minutes, cool ...

Embodiment 3

[0049] One, a kind of preparation method of Pythium oligandrum fertilizer, comprises the steps:

[0050] (1) Solid multiplication strains:

[0051] 1) Use self-isolated Pythium oligandrum strains for solid propagation: use tap water to prepare a solid medium (pH6.35), so that the prepared medium contains 1.0g / L (NH 4 ) 2 SO 4 , 0.5g / LKH 2 PO 4 , 0.5g / L MgSO 4 ,0.25g / LCaCl 2 ,0.25g / L Asparagine, 10.0g / L Glucose, 0.1g / L Vitamin B 1 , 1mL / L trace element mixed solution [every 1L aqueous solution contains 5.0mg MnSO 4 ,5.0mg FeSO 4 ,3.0mg H 3 BO 3 ,2.0mg ZnCl 2 , 0.3 mg CuSO 4 ,0.25mg (NH 4 ) 2 MoO 4 ], 20g / L agar.

[0052] 2) Sterilize the prepared solid culture based on steam at 121°C and 1.5 atmospheric pressure for 30 minutes, cool to 45-50°C, take a petri dish with a diameter of 5-7cm, pour 5-8mL of sterilized culture medium, and cool to room temperature. Take the Pythium oligandrum CQ2010 preserved in the petri dish, pick the mycelium along the edge of the c...

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Abstract

The invention discloses a preparation method and an application of Pythium oligandrum microbial fertilizer. The preparation method comprises steps as follows: firstly, a Pythium oligandrum strain or a Pythium oligandrum oospore is subjected to solid propagation, and a propagated strain is obtained; the propagated strain is subjected to liquid fermentation, a fermentation broth is obtained and evenly mixed with a liquid medium used for liquid fermentation, and a mixed liquid is obtained; the mixed liquid is mixed with a crushed and sterilized carrier material, a mixture is obtained, covered with a plastic film and composted for 20-30 d, and the Pythium oligandrum microbial fertilizer is prepared. The Pythium oligandrum microbial fertilizer can promote growth of crops, vegetables and fruit trees, promote the plant growth amount to increase by 7.0% generally, can control about 60% of plant fungal diseases caused by pathogenic fungi such as phytophthora, botrytis cinerea, verticillium, alternaria, botrytis, rhizoctonia, pythium and the like and has growth promoting and disease preventing effects superior to those of existing oospore preparations.

Description

technical field [0001] The invention belongs to the technical field of soil fertilizers, and in particular relates to a preparation method and application of Pythium oligandrum fertilizer. Background technique [0002] Fertilizer is the grain of crops, and the application of chemical fertilizers is an important means to increase crop yields. However, long-term large-scale fertilization will cause a series of production and environmental problems, such as fertilizer waste, reduced utilization rate, diminishing fertilizer efficiency, serious diseases, soil compaction, and environmental pollution. At present, the utilization rate of chemical nitrogen fertilizer in my country generally does not exceed 35%, and the utilization rate of phosphorus fertilizer is even lower. Applying bacterial fertilizer to promote crop growth, maintaining or reducing the amount of fertilizer is one of the important means to improve fertilizer efficiency. At present, my country mainly produces bacte...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F17/00
CPCC05B7/00C05F17/00C05G3/00C05C3/00C05C11/00C05D3/00C05D5/00C05D9/02C05F5/002C05F9/04C05F11/08Y02W30/40
Inventor 袁玲黄建国杜如万王剑王勇陈玉蓝
Owner SOUTHWEST UNIVERSITY
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