2 results about "L-asparagine" patented technology

The invention belongs to the technical field of biology, and particularly relates to an additive composition for CHO cell culture and application of the additive composition. The composition is prepared from 0.1 g / L to 0.5 g / L of carboxymethyl-beta-cyclodextrin, 0.4 g / L to 1.2 g / L of asparagine and 0.02 g / L to 0.1 g / L of sodium pyruvate. By adding the composition, the antibody yield can be effectively increased, meanwhile, the glycosylation modification proportion of G0F is increased, and a simple, convenient and effective yield and quality control method is provided for research, development and production of antibody drugs.

This invention discloses a culture medium and process for alleviating ammonium accumulation in cells. The concentrations of the substances added to the culture medium are as follows: 2 g / L glutamate, 0 g / L glutamine, 2 g / L aspartic acid, 2 g / L asparagine, 1 g / L serine, 1 g / L pyruvate, 0.5 g / L α-ketoglutarate, 0.5 g / L oxaloacetic acid, 0.5 g / L proline, 0 g / L glucose, 8 g / L galactose, and 0.1% glycerol. Compared with the prior art, the advantages of this application are: this method mainly achieves the purpose of maintaining cell viability and reducing the cell metabolic byproduct ammonium ions by adjusting the proportion of substances related to cell metabolic pathways and adding additional cell protectants; using the optimized culture medium of this invention, the cell viability can be increased from 42.38% to 95.21% and the ammonium ion concentration can be reduced from 6.308 mM to 0.914 mM by day 7.