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Broad-spectrum compound microbial bactericide and preparation method thereof

A technology of compound microorganisms and fungicides, applied in the fields of botanical equipment and methods, fungicides, biocides, etc., can solve the problems of frequent occurrence of human and animal poisoning, increased resistance of pests, and aggravated environmental pollution, and achieves easy reproduction and promotion. The effect of growing crops and a wide range of hosts

Active Publication Date: 2016-03-02
甘肃绿能农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a broad-spectrum compound microbial fungicide to solve the problems that the existing fungicides make the environmental pollution worse, the poisoning of humans and animals occurs frequently, and the insecticide resistance of pests is enhanced.

Method used

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  • Broad-spectrum compound microbial bactericide and preparation method thereof

Examples

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Effect test

Embodiment 1

[0037] 1) Preparation of two bacterial spore powders

[0038] The activated Bacillus thuringiensis and Bacillus amyloliquefaciens slant culture strains were respectively inoculated into 500mL primary liquid seed medium according to the inoculation amount of 5%, cultured on a shaker at 28-30°C and 180 rpm, Ferment for 2 days. Then transfer to the second-stage seed shaker flask, cultivate on a shaker at 28-30°C and 180 rpm, and ferment for 2 days. Transfer to 50L bacterial fermenter culture medium again, temperature 28-30 ℃, rotating speed 180 rpm, ferment for 2 days. Transfer the strains of the culture medium of the bacterial fermenter to a 500L production tank at a temperature of 28-30°C and a rotation speed of 180 rpm, and ferment for 3 days to obtain a liquid bacterial suspension. The two kinds of bacterial suspensions are concentrated and dried at low temperature to prepare spore powder. Bacillus thuringiensis contains 10 billion effective viable bacteria / g; Bacillus amyl...

Embodiment 2

[0044] 1) Preparation of two bacterial spore powders

[0045] Two activated slant culture strains of Bacillus thuringiensis and Bacillus amyloliquefaciens were respectively inoculated into 500mL primary liquid seed medium according to the inoculum amount of 8%, cultured on a shaker at 28-30°C and 180 rpm, Ferment for 2 days. Then transfer to the second-stage seed shaker flask, cultivate on a shaker at 28-30°C and 180 rpm, and ferment for 2 days. Transfer to 50L bacterial fermenter culture medium again, temperature 28-30 ℃, rotating speed 180 rpm, ferment for 2 days. Transfer the strains of the culture medium of the bacterial fermenter to a 500L production tank at a temperature of 28-30°C and a rotation speed of 180 rpm, and ferment for 3 days to obtain a liquid bacterial suspension. The two kinds of bacterial suspensions are concentrated and dried at low temperature, and then prepared into spore powder. Bacillus thuringiensis contains 11 billion effective viable bacteria / g; ...

Embodiment 3

[0051] 1) Preparation of two bacterial spore powders

[0052] The activated Bacillus thuringiensis and Bacillus amyloliquefaciens slant culture strains were respectively inoculated into 500mL primary liquid seed medium according to the inoculation amount of 10%, cultured on a shaker at 28-30°C and 180 rpm, Ferment for 2 days. Then transfer to the second-stage seed shaker flask, cultivate on a shaker at 28-30°C and 180 rpm, and ferment for 2 days. Transfer to 50L bacterial fermenter culture medium again, temperature 28-30 ℃, rotating speed 180 rpm, ferment for 2 days. Transfer the strains of the culture medium of the bacterial fermenter to a 500L production tank at a temperature of 28-30°C and a rotation speed of 180 rpm, and ferment for 3 days to obtain a liquid bacterial suspension. The two bacterial suspensions were concentrated and dried at low temperature to prepare spore powder. Bacillus thuringiensis contained 11.3 billion viable bacteria / g; Bacillus amyloliquefaciens ...

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Abstract

The invention discloses a broad-spectrum composite microbial bactericide and a preparation method thereof. The original composite spore powder is composed of the following three microorganisms: Bacillus thuringiensis ( Bacillus? Thuringiensis ) GSICC? 32807, Bacillus amyloliquefaciens ( Bacillus? ? amyloliquefaciens ? ) GSICC? 32826 and Pythium oligandrum ( Pythium oligandrum ) GSICC? 61615. Mix two kinds of bacterial spore powders and one kind of fungal spore powders according to the weight ratio of 1:1:1 to make a broad-spectrum compound microbial fungicide product. The broad-spectrum compound microbial fungicide product provided by the invention can quickly colonize the root circle of plants, and can significantly prevent, antagonize and kill pathogenic bacteria by competing with pathogenic bacteria for infection sites, reparasitizing, secreting auxin, and promoting plant nutrient absorption. A variety of crop microbial diseases; at the same time, it has the effect of promoting crop growth, increasing yield and improving quality.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and relates to a broad-spectrum composite microbial bactericide and a preparation method thereof. Background technique [0002] The global economic losses caused by crop diseases and insect pests are huge. Chemical pesticides have played a huge role in promoting the prevention and control of diseases and insect pests and the development of agricultural production. Enhance pest resistance. Therefore, the development and use of high-efficiency and broad-spectrum environmentally friendly biopesticides is of great significance to the sustainable development of agriculture, the protection of human health and food safety, and the maintenance of ecological environment and biodiversity. In the national medium and long-term scientific and technological development program, "creation of environmentally friendly pesticides and ecological agriculture" is listed as a key area of ​​agricultural d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P1/00A01P3/00
Inventor 周剑平祝英
Owner 甘肃绿能农业科技股份有限公司
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