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A kind of plant root rot biocontrol bacteria and application thereof

A technology of plant roots and biocontrol bacteria, applied in the direction of plant growth regulators, application, plant gene improvement, etc., to achieve the effect of increasing plant yield and reducing disease severity

Active Publication Date: 2022-02-01
瀚星生物科技(南京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In summary, the prior art still lacks a kind of green, efficient environmental probiotics for preventing plant root rot

Method used

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  • A kind of plant root rot biocontrol bacteria and application thereof
  • A kind of plant root rot biocontrol bacteria and application thereof
  • A kind of plant root rot biocontrol bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The screening of embodiment 1 bacterium Pythium oligandrum Pyo34-3

[0056] 1. Primary screening: Isolation of potential biocontrol bacteria

[0057] 1.1. Acquisition of test materials: Select well-growing soybean plants from a field where root rot occurs all year round (soybean field in Jiangning District, Nanjing City), and take their root tissue and rhizosphere soil.

[0058] 1.2. Isolation and cultivation of soybean root microorganisms: cut the soybean root tissue, rinse it three times with sterile water, cut the cleaned root tissue into 0.5-1cm segments with a sterilized scalpel, and dry it with sterile absorbent paper The water on the surface of the root tissue was placed on the V8 agar solid medium containing ampicillin, rifampicin and pentachloronitrobenzene, and placed in an incubator at 25°C for 2 days.

[0059] 1.3. Isolation and cultivation of soybean rhizosphere soil microorganisms: Take 0.25 g of soil with sterilized tweezers, put it on the V8 agar solid ...

Embodiment 2

[0081] The influence of embodiment 2 bacterium Pythium oligandrum Pyo34-3 on the root rot that different plant root rot pathogens cause

[0082] The inventor of the present invention has carried out the test of the influence of the bacteria Pythium oligandrum Pyo34-3 that a large amount of embodiment 1 screens causes to the root rot of different plant root rot pathogens, below with Phytophthora sojae, Pythium ultima, oxysporum Fusarium and Phomopsis are illustrated as examples.

[0083] 【Phytophthora soybean】

[0084] The test method for the influence of Pythium oligandrum Pyo34-3 on the plant root rot caused by Phytophthora soybean comprises the steps:

[0085] R1. Inoculate Pythium oligandrum strain Pyo34-3 and Phytophthora sojae P6497 in a 9cm-diameter petri dish containing 20mL of 10% unfiltered V8 medium, and incubate at 25°C for 4d and 7d in the dark, respectively. , until the strains are covered with petri dishes;

[0086] R2. Select a pot with a diameter of 12 cm, a...

Embodiment 3

[0116] Field test of embodiment 3 Pythium oligandrum Pyo34-3

[0117] Step 1. Inoculate Pythium oligandrum strain Pyo34-3 in a 9cm-diameter medium containing 20mL of 10% unfiltered V8 medium, and incubate at 25°C in the dark for 4 days until it covers the Petri dish.

[0118] Step 2: Sterilize the matrix (soybean straw: soybean flour: vermiculite = 100:5:10) at 121°C for 20 minutes, and put it into a sterile solid fermentation bag with air holes after cooling. Each bag of matrix is ​​1500g.

[0119] Step 3: Cut the culture medium of Pythium oligandrum Pyo34-3 into small pieces of 0.5cm×0.5cm, inoculate two pieces in each bag of matrix, add sterile water to make the moisture content in the matrix reach 70%, seal it Place it in an incubator at 28°C for 15 days in the dark to prepare the solid fermentation agent of Pythium oligandrum Pyo34-3.

[0120] Step 4, microscopic examination of the solid fermentation agent of Pythium oligandrum Pyo34-3, its oospore quantity is 1 * 10 8...

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Abstract

The invention discloses a plant root rot biocontrol bacterium and its application. The biocontrol bacterium is named Pythium oligandrum Pyo34-3, and the depository unit is: General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC NO.20224; applications include the application in the control of plant root rot caused by Phytophthora soybean, Pythium ultima, Fusarium oxysporum and Phomopsis etc. The invention provides a beneficial microorganism capable of effectively preventing and controlling plant root rot and application thereof.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a plant root rot biocontrol fungus and its application. Background technique [0002] Root rot is a plant disease that occurs in the roots of plants, resulting in underdeveloped plant roots, short and thin aerial parts, light green leaves, and significantly reduced branching and fruiting. In the initial stage of root rot, only individual branch roots and fibrous roots are infected, and gradually expand to the main root. After the main root is infected, the early plants do not show symptoms. Later, as the degree of root rot intensifies, the function of the plant to absorb water and nutrients gradually weakens, and the leaves of the plant wilt. When the condition is severe, the root bark turns brown and separates from the pith, the leaves wilt, and the plant stumbles and dies. Root rot is a major serious disease in my country's agricultural production. It occurs in almost all...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N15/31A01N63/30A01P3/00C05G3/60C05F11/08C12R1/645
CPCC12N1/14C07K14/37A01N63/30C05G3/60C05F11/08C12R2001/645C12N1/145
Inventor 郑小波杨波王源超张正光叶文武柳泽汉孙哲冯慧王晓莉
Owner 瀚星生物科技(南京)有限公司
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