Biocontrol Strain bf6 for Controlling Tomato Yellow Leaf Curl Virus and Its Application

A technology of tomato yellowing curved leaves and bio-control strains, applied in the field of agricultural microorganisms, can solve the problems of high cost, limited resistant materials, and difficulty in large-scale promotion, and achieve the effect of saving costs and reducing residues

Active Publication Date: 2014-10-22
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Agricultural measures and chemical control methods are mainly used in production to prevent and control, the cost is high, the effect is not ideal, and even negatively affects the environment
Although cultivating disease-resistant varieties is the best way to prevent and control tomato yellow leaf curl virus disease, it is difficult to breed due to the complex inheritance of resistance to tomato yellow leaf curl virus disease and the limited commercially valuable resistant materials Breeding lines with stable resistance
Moreover, most of the existing disease-resistant varieties are regional, and it is not easy to promote them on a large scale.

Method used

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  • Biocontrol Strain bf6 for Controlling Tomato Yellow Leaf Curl Virus and Its Application
  • Biocontrol Strain bf6 for Controlling Tomato Yellow Leaf Curl Virus and Its Application
  • Biocontrol Strain bf6 for Controlling Tomato Yellow Leaf Curl Virus and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Screening method: Pseudomonas BF6 was isolated from tomato rhizosphere soil in tomato greenhouses in Gaoyou Town, Gaoyou City, Yangzhou City. Gently brush the soil attached to the tomato root surface with a soft brush, weigh 3g and add it to 27ml of sterilized 0.85% NaCl, oscillate on a shaker at 180rmp for 0.5h, let it stand for 5min, to obtain a 10-1 dilution, and absorb the supernatant Solution 0.1mL was diluted sequentially, and 10 -4 、10 -5 、10 -6 Apply 0.1 mL each of the three gradient dilutions on the LB plate, incubate at 28°C for 48 hours, pick a single colony and inoculate it into fresh LB solid medium, and obtain purified strains through repeated transfer. The purified strains were stored in 40% glycerol in a -70°C ultra-low temperature freezer.

[0023] Identification method: The strain was identified through morphological characteristics, physiological and biochemical experiments and 16S rDNA sequence analysis.

[0024] Morphological characteristics: Gr...

Embodiment 2B

[0031] The preparation of embodiment 2BF6 biocontrol agent

[0032] After inoculating Pseudomonas BF6 (CGMCC NO.7238) into LB culture medium for 16 hours at 30°C with shaking at 200rpm, take samples in the ultra-clean workbench every 2 hours to measure its OD value at 600nm. When the OD value is between 0.5- At 0.8 o'clock, the cultivation was finished, and this bacterial solution was used as the seed bacterial solution. Inoculate the seed bacteria liquid into the LB fermentation culture medium at a volume ratio of 1:500 for fermentation and culture, and cultivate at 30°C and 200rpm for 24 hours. The pH value of the fermentation liquid is maintained at 7.0, the dissolved oxygen is 20%, and the ventilation rate is 5-7m 3 / hour, tank pressure 0.05-0.1KPa. Then centrifuge at 6000rpm for 10min, take the precipitate and dilute it with sterilized water to make bacterial preparation, the total concentration of viable bacteria in the finished bacterial preparation is 1×10 9 -1×10 ...

Embodiment 3

[0033] Example 3 Greenhouse Test Measures the Control Effect of BF6 on Tomato Yellow Leaf Curl Virus Disease

[0034] The tomato variety is "He He 903", which is transplanted at the 4-leaf stage and sprayed 5 days after transplanting. Get the bacterium preparation prepared in Example 2, dilute with water to the final concentration of bacterium liquid and be 5 * 10 7 CFU / mL, the spray standard is the distribution of mist droplets on each leaf, subject to not falling. The tomato yellow leaf curl virus was inoculated on the 7th day after the biocontrol agent treatment. The second same treatment was carried out 15 days after the first treatment. The treatment of inoculating virus alone without spraying biocontrol bacteria was used as the control treatment. There were 24 tomato plants in each treatment group, with three repetitions. The tomatoes were cultivated in a greenhouse with a temperature of 28°C and a light of 12h / 12h. When the disease was detected, the disease grade wa...

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Abstract

The invention belongs to the technical field of agricultural microbe, and relates to a biocontrol strain BF 6 for preventing and treating tomato yellow leaf curl virus and application thereof. The biocontrol strain BF 6 is identified to be pseudomonas sp. The biocontrol strain BF 6 for preventing and treating tomato yellow leaf curl virus is preserved in common microorganism center of China Microbial Strain Preserving Management Committee on 27th, January, 2013, the strain preserving number is CGMCC No.7238. The strain can significantly control the tomato yellow leaf curl virus, reaching up to 59.51%. Field experiments show that the biocontrol strain BF 6 can significantly reduce the disease severity of the tomato yellow leaf curl virus disease with preventing efficiency of 50.79%-53.72%. The yield-increasing effect is more than 28%. The biocontrol strain BF 6 and especially prepared fungicide can be applied in the tomato yellow leaf curl virus disease control and yield increasing aspects.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a biocontrol bacterial strain BF6 for preventing and treating tomato yellow leaf curl virus disease and an application thereof. Background technique [0002] Tomato yellow leaf curl virus disease is caused by tomato yellow leaf curl virus (TYLCV) belonging to the genus Begomovirus in the family Geminiviridae. TYLCV was first discovered in 1939 along the Jordan River in Israel and was officially named in 1964. In the following 30 years, the virus spread rapidly to many countries and regions such as the Middle East, the Mediterranean coast, Africa, Europe, America, Asia and Australia. Compared with other viral diseases, tomato yellow leaf curl virus has the characteristics of rapid mutation, rapid expansion, strong harm and difficult treatment. It is called the "cancer" of plants and is a devastating disease in tomato production. disease. [0003] Since the 1990s, it has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P21/00C12R1/38
Inventor 刘红霞郭坚华丁雪玲
Owner NANJING AGRICULTURAL UNIVERSITY
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