Method for raising disease resistance of paddy rice to rice blast by utilization of alginooligosaccharide
A technology of alginic acid oligosaccharides and sodium alginate oligosaccharides, applied in botany equipment and methods, chemicals for biological control, applications, etc., can solve problems such as drug residues and ecological environment pollution, and achieve growth promotion, The method is simple and easy, and the effect of improving the activity of defense enzymes
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Embodiment 1
[0026] (1) Sodium alginate oligosaccharide preparation: Take 1 kg of sodium alginate, dissolve it in 100 liters of water, add 10 liters of 30% hydrogen peroxide, react at 80°C for 90 minutes, freeze-dry for 48 hours, and grind into powder .
[0027] (2) Preparation of sodium alginate oligosaccharide solution: 100 g of prepared sodium alginate oligosaccharide was dissolved in 400 liters of water to prepare a solution.
[0028] (3) Spraying: The sodium alginate oligosaccharide solution is sprayed when the rice seedlings grow to four leaves and one heart in the experimental field of Dalian, Liaoning, and are about 15-20cm high. The spraying amount is the volume (ml) of the sodium alginate oligosaccharide solution: Rice area (m 2 )=100:1.
[0029] It can be seen from Table 1 that after spraying with sodium alginate oligosaccharide solution, the defense enzyme activities including phenylalanine ammonia lyase, peroxidase, and catalase in rice seedlings were significantly higher th...
Embodiment 2
[0031] (1) Sodium alginate oligosaccharide preparation: Take 1 kg of sodium alginate, dissolve it in 100 liters of water, add 10 liters of 30% hydrogen peroxide, react at 80°C for 90 minutes, freeze-dry for 48 hours, and grind into powder .
[0032] (2) Preparation of sodium alginate oligosaccharide solution: 100 g of prepared sodium alginate oligosaccharide was dissolved in 1000 liters of water to prepare a solution.
[0033] (3) Spraying: The sodium alginate oligosaccharide solution is sprayed when the rice seedlings grow to four leaves and one heart in the experimental field of Dalian, Liaoning, and are about 15-20cm high. The spraying amount is the volume (ml) of the sodium alginate oligosaccharide solution: Rice area (m 2 )=1000:1.
[0034] It can be seen from Table 1 that after spraying with sodium alginate oligosaccharide solution, the defense enzyme activities including phenylalanine ammonia lyase, peroxidase, and catalase in rice seedlings were significantly higher ...
Embodiment 3
[0035] Embodiment 3 (blank sample)
[0036] Water spraying: routine water spraying treatment was carried out when the rice seedlings grew to four leaves and one center in the experimental field in Dalian, Liaoning, and were about 15-20 cm high.
[0037] Table 1 Effect of sodium alginate oligosaccharides on defense enzymes in rice seedling leaves
[0038]
[0039]
[0040] Table 2 Control effect of sodium alginate oligosaccharides on rice blast
[0041] Detection Indicator
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