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Rhodopseudomonas palustris strains, application of rhodopseudomonas palustris strains, fungicide pesticide as well as preparation method and application of fungicide pesticide

A technology of rhodopseudomonas marsh and strains, applied in the fields of botany equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of difficult control of rice false smut, prominent chemical resistance, pesticide residues, etc., to achieve Improve crop disease resistance, improve defense enzyme activity, and reduce production costs

Active Publication Date: 2014-04-16
湖南新长山农业发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims to overcome the deficiencies of the prior art and provide a low production cost, non-toxic, green, The easy-to-use strains of Rhodopseudomonas palustris and the corresponding bacterial agents and medicaments prepared therefrom, and the preparation method and application of the corresponding bacterial agents and medicaments are also provided, which can prevent and treat rice false smut and improve the disease resistance of rice

Method used

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  • Rhodopseudomonas palustris strains, application of rhodopseudomonas palustris strains, fungicide pesticide as well as preparation method and application of fungicide pesticide
  • Rhodopseudomonas palustris strains, application of rhodopseudomonas palustris strains, fungicide pesticide as well as preparation method and application of fungicide pesticide
  • Rhodopseudomonas palustris strains, application of rhodopseudomonas palustris strains, fungicide pesticide as well as preparation method and application of fungicide pesticide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040]A strain of Rhodopseudomonas palustris of the present invention, its name is Rhodopseudomonas palustris CS-1, specifically a strain of photosynthetic bacteria, and its preservation number in the China Center for Type Culture Collection is CCTCC No: M 2012224, the depository address is located at Wuhan University, China, and the deposit date is June 12, 2012. The initial sample of the bacterial strain of the present invention was collected on November 2, 2010 in a dry pond in Hunan Academy of Agricultural Sciences. When the initial sample was collected, in order to maintain the anaerobic environment and the freshness of the sample, the sediment at a depth of 3cm to 4cm in the pond sludge and the mixture of pond mud and water were intercepted. Take 5ml of the collected water sample, 5ml of the cement sample after precipitation, and 5ml of the sludge sample after mixing with an appropriate amount of sterile water. 7500Lx, temperature is 30 ℃ light incubator constant temper...

Embodiment 2

[0046] Example 2: biocontrol agent for Rhodopseudomonas palustris CS-1 and its preparation method.

[0047] A biocontrol agent for Rhodopseudomonas palustris strains, which is obtained after activation, seed cultivation and production cultivation of the Rhodopseudomonas paluscans strains obtained in the above-mentioned embodiment 1.

[0048] The specific preparation method of the biocontrol agent of the present embodiment comprises the following steps:

[0049] (1) Activation: the cryopreservation species of Rhodopseudomonas palustris CCTCC M 2012224 of embodiment 1 is cultivated by the double-layer plate culture method, and it is cultivated until red single colony occurs; the photosynthetic bacteria solid medium that adopts is: (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.02%, NaHCO 3 0.5%, K 2 HPO 4 0.05%, NaCl 0.02%, yeast extract 0.15% and agar 1.5%, pH7.0~7.2;

[0050] (2) Seed culture: Put the red single colony cultivated in step (1) into a serum bottle with a volume of about 12...

Embodiment 3

[0054] Example 3: biocontrol agent for Rhodopseudomonas palustris CS-1 and its preparation method.

[0055] A biocontrol agent for Rhodopseudomonas palustris strain of the present invention, the biocontrol agent is the fermentation supernatant obtained after fermentation and centrifugation of the biocontrol agent obtained in Example 2.

[0056] The preparation method of the biocontrol agent of this embodiment specifically includes the following steps: take the biocontrol agent obtained in Example 2 and ferment and cultivate it for 6d to 8d to obtain the fermented liquid of Rhodopseudomonas palustris CCTCC M 2012224, and heat it at 8000rpm Centrifuge for 2 minutes under the same conditions, collect the supernatant after centrifugation, and filter the supernatant after centrifugation with a bacterial filter to obtain a sterile fermentation supernatant without photosynthetic bacteria cells, and store it at 4°C for future use.

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Abstract

The invention discloses a rhodopseudomonas palustris CCTCC M 2012224, and application of the rhodopseudomonas palustris CCTCC M 2012224 in prevention and control of rice false smut or improvement of catalase activity in the rice body, and further discloses an antagonistic fungicide obtained by activation, seed culture and production culture of the strains. Fermentation supernate obtained through fermentation and centrifugation of the antagonistic fungicide can be used as an antagonistic pesticide; thallus crushing liquid obtained by fermentation culture and crushing of the antagonistic fungicide can also be used as the antagonistic pesticide. The antagonistic fungicide and the antagonistic pesticides obtained both can be used for preventing and controlling the rice false smut and improving the catalase activity in the rice body. The rhodopseudomonas palustris CCTCC M 2012224, the antagonistic fungicide and the antagonistic pesticides have the advantages of low production cost, nontoxicity, environmental friendliness and the like, is convenient to use, and can be used for preventing and controlling the rice false smut and improving the disease resistance of rice.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to the application of a strain of Rhodopseudomonas palustris and its medicament in preventing and treating rice diseases. Background technique [0002] Rice is one of the main food crops in my country, but the continuous occurrence of rice diseases has always affected rice production. Rice false smut is one of the main diseases of rice. It is widely distributed in the world's rice production areas and has a tendency to increase year by year. Rice diseases caused by rice false smut can cause direct economic losses of hundreds of millions of yuan every year. Therefore, research and control of rice Rice false smut is urgent. [0003] At present, the control of rice false smut mainly adopts the method of chemical pesticide control. Although chemical pesticides have the advantages of convenient application and high control effect, the excessive use of chemical pestici...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
Inventor 刘勇陈莎张松柏张德咏罗香文彭静程菊娥谭新球成飞雪朱春晖杨春晓
Owner 湖南新长山农业发展股份有限公司
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