Brassica napus seed initiator, initiation method and application
A technology of Brassica napus and initiator, applied in the direction of seed and rhizome treatment, application, botany equipment and methods, etc., can solve the problems of lagging research on drought resistance of rapeseed, and achieve the goal of enhancing defense enzyme activity, saving dosage, and neat emergence Effect
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Embodiment 1
[0036] (1) Preparation of initiator:
[0037] Formula 1: calcium chloride 5g / L, chitosan oligosaccharide 15g / L, sucrose 5g / L, proline 2g / L.
[0038] Formula 2: calcium chloride 3g / L, chitosan oligosaccharide 15g / L, sucrose 5g / L, proline 2g / L.
[0039] Formula 3: calcium chloride 8g / L, chitosan oligosaccharide 15g / L, sucrose 5g / L, proline 2g / L.
[0040] Formula 4: calcium chloride 5g / L, chitosan oligosaccharide 10g / L, sucrose 5g / L, proline 2g / L.
[0041] Formula 5: calcium chloride 5g / L, chitosan oligosaccharide 20g / L, sucrose 5g / L, proline 2g / L.
[0042] Formula 6: calcium chloride 5g / L, chitosan oligosaccharide 15g / L, sucrose 3g / L, proline 2g / L.
[0043] Formula 7: calcium chloride 5g / L, chitosan oligosaccharide 15g / L, sucrose 10g / L, proline 2g / L.
[0044] Formula 8: calcium chloride 5g / L, chitosan oligosaccharide 15g / L, sucrose 5g / L, proline 1g / L.
[0045] Formula 9: calcium chloride 5g / L, chitosan oligosaccharide 15g / L, sucrose 5g / L, proline 3g / L.
[0046] (2) Soaking o...
Embodiment 2
[0057] (1) Preparation of initiator: calcium chloride 5g / L, chitosan oligosaccharide 15g / L, sucrose 5g / L, proline 2g / L.
[0058] (2) Soaking of Brassica napus seeds in an initiator: put the Brassica napus seeds in a glass container, add the prepared initiator, and the dosage of the initiator is 3 times the mass of the seeds. The container was placed in a dark incubator at 20° C. for 12 h, during which it was stirred slowly 3 times.
[0059] (3) Re-drying treatment of Brassica napus seeds: Take out Brassica napus seeds and wash them with distilled water for 20 seconds, filter off excess water, blot dry with absorbent paper and put them in a blast drying oven at 25°C to keep the moisture content of Brassica napus seeds at 9%, the dried seeds should be stored in a light-proof environment below 4°C. This processing is denoted as T2.
Embodiment 3
[0071] (1) Preparation of initiator: calcium chloride 5g / L, chitosan oligosaccharide 15g / L, sucrose 5g / L, proline 2g / L.
[0072] (2) Soaking of Brassica napus seeds in an initiator: put the Brassica napus seeds in a glass container, add the prepared initiator, and the dosage of the initiator is 3 times the mass of the seeds. The container was placed in a dark incubator at 20° C. for 12 h, during which it was stirred slowly 3 times.
[0073] (3) Re-drying treatment of Brassica napus seeds: Take out Brassica napus seeds and wash them with distilled water for 20 seconds, filter off excess water, blot dry with absorbent paper and put them in a blast drying oven at 25°C to keep the moisture content of Brassica napus seeds at 9%, the dried seeds should be stored in a light-proof environment below 4°C. Denote it as T2.
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