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Streptomyces albidoflavus strain as well as application thereof in pesticide

A technology of Streptomyces lividans and microbial pesticides, applied in the field of microorganisms, can solve the problems of poor fermentation performance of strains and poor field test results, and achieve the effect of inhibiting germination

Active Publication Date: 2017-10-24
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these soil fungal disease biocontrol strains have strong antifungal activity, the field test effect is poor, and many strains have poor fermentation performance, and there are usually certain geographical limitations in application

Method used

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  • Streptomyces albidoflavus strain as well as application thereof in pesticide
  • Streptomyces albidoflavus strain as well as application thereof in pesticide
  • Streptomyces albidoflavus strain as well as application thereof in pesticide

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Isolation, Identification and Biological Characterization of Streptomyces microalbicans W68 for Controlling Soilborne Fungal Diseases

[0052] 1. Isolation and purification of Streptomyces microalbicans W68 for the control of soil-borne fungal diseases

[0053] In this experiment, several strains of actinomycetes were isolated from marine activated sludge. The process is as follows: add 1g of sample to a sterilized 300ml Erlenmeyer flask filled with small glass beads and 99ml of sterile saline, and incubate on a shaker at 28°C for 1h . Take 1ml of the suspension and dilute to 10 by gradient dilution method -4 , take stock solution, 10 -2 Diluent and 10 -4 The dilution solution was coated with potassium dichromate at a final concentration of 50ug / ml Gaoshi No. 1 medium, and single colonies were picked on the 5th day, 7th day and 10th day, and then streaked on the PDA medium for purification and isolation.

[0054] The resulting purified strain was stored at -80°C wi...

Embodiment 2

[0072] Antifungal activity test of Streptomyces albus W68 isolated from deep-sea sludge to control soil-borne fungal diseases

[0073] Determination of the antifungal activity of Streptomyces albus W68 for the control of soil-borne fungal diseases by plate confrontation method:

[0074] (1) Strain cultivation and collection: Streptomyces albiflous W68 for the prevention and treatment of soil-borne fungal diseases is inoculated on a PDA plate by streaking method, cultivated at 28°C for 5-7 days until sufficient spores are produced; scrape the spores into appropriate amount of sterile water Make a spore suspension for later use.

[0075] (2), the cultivation of pathogenic bacteria: inoculate cucumber blight, cabbage wilt, wheat root rot, rice blast fungus, cotton fusarium wilt, and cotton Verticillium dahliae on the PDA plate, and use it after cultivating for one week at 28°C.

[0076] (3), plate confrontation experiment: respectively inoculate the bacterial block of pathogenic...

Embodiment 3

[0078] Chitinase activity test of Streptomyces albus W68 isolated from deep-sea sludge to control soil-borne fungal diseases

[0079] (1) Preparation of colloidal chitin: Weigh 10 g of chitin sample, slowly add 200 ml of concentrated hydrochloric acid (operated in a fume hood), and stir evenly while adding. After standing at 4 degrees for 24 hours, use glass wool to filter into 600ml of pre-cooled 50% ethanol, and continue to stand at 4 degrees for 24 hours. Then centrifuge at 3000rpm for 20min*3 times, wash with water to neutral pH, and finally dissolve in 200ml water. Made into 5% colloidal chitin, adjusted the pH to neutral with NaOH, sterilized under high temperature and high pressure at 115°C for 20min, and stored in the dark at 4°C.

[0080] (2) Determination of chitinase activity: Pick a single colony from the W68 strain that has been grown on PDA medium for 5-7 days, and inoculate it into 0.4% chitin with chitin as the only carbon source for screening On the petri di...

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Abstract

The invention discloses a streptomyces albidoflavus W68 (with the strain collection number of CGMCC No. 12210) which has broad spectrum antifungal activity and is separated from deep sea sludge as well as an application technology of a microbial pesticide prepared from the strain and belongs to the technical field of microorganisms. The application technology of the microbial pesticide which is prepared from the streptomyces albidoflavus W68 separated from deep sea sludge and contains strain active spores and active enzymes and active secondary metabolites produced by the strain is simple, convenient, efficient and environment-friendly, and the concentration of spores of a solid fermented bacterium preparation can reach 10 billion. Pot experiments prove that the streptomyces albidoflavus W68 pesticide preparation separated from deep sea sludge shows remarkable control effects on soil-borne fungal diseases such as wheat root rot, melon fusarium wilt, phytophthora capsici and wheat take-all.

Description

technical field [0001] The invention relates to a Streptomyces albicans for preventing and controlling soil-borne fungal diseases from the ocean and its application in pesticides, belonging to the application of marine microorganisms and the technical field of microorganisms such as crop disease prevention and control. Background technique [0002] Fungal diseases are the main cause of crop yield and quality reduction. The frequent use of chemical pesticides has led to a sharp increase in the resistance of some pathogenic fungi, pollution of the environment and agricultural products, and damage to the balance of microorganisms in and out of plants, which has exacerbated the outbreak of diseases. [0003] The research on the prevention and control of soil-borne plant fungal diseases has always been a hot and difficult point, and the fungicides on the market generally have no or little effect on it. Over the years, people have also screened some biocontrol bacteria resistant ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/20C12N1/205C12R2001/465
Inventor 李少杰张振颖孙宪昀
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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