Preparation method of fluorescent pseudomonas microbial pesticide

A technology of Pseudomonas fluorescens and microbial pesticides, applied in the field of microbial pesticides, can solve the problems of unstable effect and unstable activity of rape sclerotinia, and achieve the goal of preventing and controlling rape sclerotinia and inhibiting sclerotia Easy to form and produce

Inactive Publication Date: 2009-12-23
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the field promotion experiment, the effect of preventing and controlling Sclerotinia sclerotiorum was unstable due to the unstable activity of the bacteria.

Method used

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  • Preparation method of fluorescent pseudomonas microbial pesticide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The preparation method steps of Pseudomonas fluorescens microbial pesticide are as follows:

[0056] (1) Prepare liquid culture medium, get following weight ratio material and evenly mix:

[0057] Peptone 20g; Glycerin 10ml; K 2 HPO 4 ·3H 2 O 1.5 g; MgSO 4 ·7H 2 O 1.5 g; distilled water 1000 ml;

[0058] (2) Inoculate 1 milliliter of Pseudomonas fluorescens P13 bacterial suspension into 100 milliliters of liquid culture medium with a pipette gun in the ultra-clean workbench, put it into a constant temperature shaker, rotate at a speed of 200 rpm, and shake for 24 hours;

[0059] (3) Transfer the thalline suspension after shaking culture into a sterilized centrifuge tube, put into a refrigerated centrifuge with a rotation speed of 8000 rpm for 10 minutes, and precipitate to obtain P13 thalline;

[0060] (4) Take 1 gram of sodium carboxymethylcellulose, add 99 grams of distilled water, stir evenly, and sterilize at a temperature of 121° C. for 30 minutes;

[0061] ...

Embodiment 2

[0066] The preparation method steps of Pseudomonas fluorescens microbial pesticide are as follows:

[0067] (1) Prepare liquid culture medium, get following weight ratio material and evenly mix:

[0068] 40 grams of peptone; 20 ml of glycerin; K 2 HPO 4 ·3H 2 O 3 g; MgSO 4 ·7H 2 O 3 grams; 2000 milliliters of distilled water;

[0069] (2) Inoculate 10 milliliters of Pseudomonas fluorescens P13 strain into 1000 milliliters of liquid culture medium with a pipette gun in the ultra-clean workbench, put it into a constant temperature shaker, rotate at a speed of 200 rpm, and shake for 24 hours;

[0070] (3) Transfer the thalline suspension after shaking culture into a sterilized centrifuge tube, put into a refrigerated centrifuge with a rotation speed of 8000 rpm for 10 minutes, and precipitate to obtain P13 thalline;

[0071] (4) Get 1 gram of sodium carboxymethylcellulose, add 99 grams of distilled water, stir evenly, and sterilize at a temperature of 121° C. for 30 minutes...

Embodiment 3

[0077] The preparation method steps of Pseudomonas fluorescens microbial pesticide are as follows:

[0078] (1) Prepare liquid culture medium, get following weight ratio material and evenly mix:

[0079] 40 grams of peptone; 20 ml of glycerin; K 2 HPO 4 ·3H 2 O 3 g; MgSO 4 ·7H 2 O 3 grams; 2000 milliliters of distilled water;

[0080] (2) Use a pipette gun to inoculate 10 milliliters of Pseudomonas fluorescens P13 strain into 1000 milliliters of liquid culture in the ultra-clean workbench, put it into a constant temperature shaker, rotate at a speed of 200 rpm, and shake for 24 hours;

[0081] (3) Transfer the thalline suspension after shaking culture into a sterilized centrifuge tube, put into a refrigerated centrifuge with a rotation speed of 8000 rpm for 10 minutes, and precipitate to obtain P13 thalline;

[0082] (4) Take 1 gram of sodium carboxymethylcellulose, add 99 grams of distilled water, stir evenly, and sterilize at a temperature of 121° C. for 30 minutes; pr...

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Abstract

The invention belongs to microbial pesticide, in particular to a preparation method of fluorescent pseudomonas microbial pesticide. Rape sclerotiniose is one of the greatest diseases damaging the production and the yield of rapes, the prior art adopts agricultural prevention and treatment, chemical prevention and treatment and microbial pesticide prevention and treatment, but all the effects are unsatisfactory. The preparation method of the fluorescent pseudomonas microbial pesticide comprises the following steps: taking peptone, glycerol and the like to prepare a liquid culture medium; using a pipettor to inoculate a fluorescent pseudomonas P13 strain into the liquid culture medium and carrying out constant-temperature shaking culture; carrying out refrigerated centrifugation precipitation to prepare a P13 thallus; taking the peptone, the glycerol, agar and the like to prepare a solid culture medium; inoculating the P13 thallus into the solid culture medium and carrying out constant-temperature culture at 28 DEG C; adding the solid culture medium into a container with kieselguhr and stirring the mixture; and sealing the container and standing the container for 1 to 2 days at room temperature. The invention has the advantages of obvious effects of preventing and treating rape sclerotiniose, growth promotion for rapes, no toxic or side effect, good safety to people and livestock, simple preparation technique, low cost and simple and convenient use method.

Description

Technical field: [0001] The invention belongs to microbial pesticides, in particular to a preparation method of Pseudomonas fluorescens microbial pesticides. Background technique [0002] my country is a big country of rapeseed. Sclerotinia sclerotiorum is one of the biggest diseases that harm the production and yield of rapeseed. Every year, the production reduction caused by sclerotinia in my country is as high as 30% to 80% of the total output. The prior art adopts the combination of agricultural control and chemical control, but the effects are all unsatisfactory. Agricultural prevention and control treats the symptoms but not the root cause, and chemical prevention and control causes pollution and harm to the environment. [0003] Modern biopesticides, especially microbial pesticides, are developing rapidly. So far, it has been reported that the biological control of Sclerotinia sclerotiorum by using Shell mold and Bacillus spp. has been reported. Screening of actinom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00C12N1/20C12R1/39
Inventor 肖明李慧
Owner SHANGHAI NORMAL UNIVERSITY
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