Tissue culture propagation method for Asparagus cochinchinesnsis

A technology of asparagus and tissue, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of inability to provide a large number of provenances, expand area planting inhibition, etc., to increase the speed of multiplication, shorten production time and cost Effect

Inactive Publication Date: 2012-06-13
重庆市秀山红星中药材开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the propagation techniques of asparagus mainly include seed propagation, split-root propagation and tissue culture propagation, etc. These propagation methods are just in their infancy, and there is almost no effective experience in successful practical application. Therefore, it is impossible to provide a large number of provenance sources, and expanding the planting area is limited. inhibition

Method used

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  • Tissue culture propagation method for Asparagus cochinchinesnsis
  • Tissue culture propagation method for Asparagus cochinchinesnsis

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Cut 100 spring twigs of asparagus, soak them in an aqueous solution of detergent for 5 minutes, and then rinse them with tap water; disinfect them with 75% alcohol for 10 seconds on an ultra-clean workbench, rinse them with tap water, and cut them into pieces. 500 branches of 1-2cm long stems containing 2 axillary buds, then sterilized with 0.1% mercuric chloride for 15 minutes, and finally rinsed with sterile water for 5 times; Remove the necrotic part of the wound. Then inoculate the twig stem section on the induction medium, inoculate 3 branches in every bottle, find that a small amount of callus begins to germinate in the twig cut after 5 days, and light yellow compact callus occurs after another 5 days. Then inoculate the light yellow granule callus with the good growth status of the induction in the subculture medium, inoculate 3 branches in every bottle, and carry out subculture, after 8 days, it can be observed that there are new ones in the subculture medium. ...

Embodiment 2

[0035] Cut 50 twigs of asparagus in spring, soak them in an aqueous solution of detergent for 5 minutes, and then rinse them with tap water; disinfect them with 75% alcohol on an ultra-clean workbench for 10 seconds, rinse them with tap water, and cut them into pieces. 300 branches of 1-2cm long stems containing 2 axillary buds, then sterilized with 0.1% mercuric chloride for 15 minutes, and finally rinsed with sterile water for 5 times; Remove the necrotic part of the wound. Then inoculate the twig stem section on the induction medium, inoculate 3 branches in every bottle, observe after 7 days and find that a small amount of callus begins to germinate at the twig cut, and after 5 days, a light yellow and compact callus occurs. Then inoculate the light yellow granular callus with good growth state induced in the subculture medium, inoculate 3 branches in every bottle, and carry out subculture. After 7 days, it is observed that there are new calluses in the subculture medium. ...

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PUM

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Abstract

The invention provides a tissue culture propagation method for Asparagus cochinchinesnsis. The method comprises the steps of introduction and sterilization, tissue induction, subculture, differentiation culture, strong seedling culture and rooting culture. According to the invention, through tissue culture, bulk production of seedlings can be realized, and it only takes 3 to 4 months to obtain rooted seedlings by culturing twigs of Asparagus cochinchinesnsis; therefore, production time and cost for seedlings are substantially reduced, a propagation speed is improved, and standardized and scale development of cultivation of Asparagus cochinchinesnsis can be realized.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a tissue culture propagation method of asparagus. Background technique [0002] Asparagus cochinchinensis (Lour.) Merr., also known as asparagus, is an evergreen vine of Liliaceae, and its roots are used as medicine. It is mainly produced in Guizhou, Guangxi, Yunnan, Gansu, Anhui, Henan, Hunan, Hubei, Sichuan and Jiangxi. Asparagus was first recorded in "Shen Nong's Materia Medica", and it has been recorded in successive dynasties of herbal medicines. It is sweet, bitter, cold in nature, belongs to the lung and kidney meridian, can nourish yin and moisten dryness, clear lung and generate body fluid; it is mainly used for dry cough due to dryness of the lung, cough due to consumptive fatigue, thirsty body wound, insomnia due to complex heart, internal heat and thirst, constipation due to dryness of the intestine, etc. disease. It can also treat dry throat and thirst, dry cough with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何全慧万联新杨再江祝利赵勇彪
Owner 重庆市秀山红星中药材开发有限公司
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