Method for raising directly transplantable seedlings of dendrobium candidum

A direct technology of Dendrobium officinale, applied in the biological field, can solve the problems of seedlings not strong enough, long growth cycle, high seedling cost, etc., and achieve the effect of improving quality, good light transmission performance, and low infection rate

Inactive Publication Date: 2012-06-20
YUEQING DINGYUAN BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the seedlings cultivated through the traditional tissue culture method of Dendrobium candidum, especially the tissue culture method of Dendrobium officinale, are not strong enough and have weak vitality, so they need to be transplanted into the natural environment for 3-5 months in a greenhouse. The training (hardening) of seedlings, and the longer growth cycle after transplanting, resulting in higher cost of raising seedlings

Method used

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  • Method for raising directly transplantable seedlings of dendrobium candidum
  • Method for raising directly transplantable seedlings of dendrobium candidum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In the first stage, after the capsule seeds of the mature and strong Dendrobium officinale are sterilized, in a sterile environment, they are sowed into 1 / 2MS basic medium+taro juice 15% (V / V)+agar 4g The transparent plastic cup body 1 of the culture medium of / L carries out germination culture, and the cup mouth of plastic cup body 1 is plastic-sealed by transparent plastic film 2, and filter membrane 3 is arranged on plastic film 2. Finally, it is sent to the sterilized culture room for cultivation. The sterilized room adopts natural light, with lighting tiles and sunshade nets on the top, and temperature and humidity adjustment equipment inside. The culture temperature is 24-26°C, the humidity is 65-75%, the illuminance is 1000-2000lx, and the light time is 10-12 hours / day. After 45-60 days of cultivation, the explants germinate to form green protocorms.

[0025] In the second stage, put the protocorm in a sterile environment, and transfer it to MS basic medium + BA ...

Embodiment 2

[0029] In Example 1, when the volume ratio of the special medium I formula is 1 / 2MS basic medium+taro juice 25% (V / V)+agar 6g / L, the pH value is 5.8 or 1 / 2MS basic medium+ Taro juice 20% (V / V) + agar 5g / L, pH value 5.5;

[0030] Or the volume ratio of the special medium II formula is MS basic medium + BA (6-bianaminoadenine) 0.2mg / L + agar 6g / L + potato juice 10% (V / V) + banana juice 14% (V / V) , PH value is 5.8 or MS basic medium + BA (6-Bian aminoadenine) 0.15mg / L + agar 5g / L + potato juice 8% (V / V) + banana juice 12% (V / V), PH Value is 5.5;

[0031] Or the volume ratio of the special medium III formula is MS basic medium + NAA (α-naphthalene acetic acid) 0.2mg / L + banana juice 10% (V / V) + potato juice 7% (V / V) + taro juice 12% ( V / V) + agar 6g / L, PH value is 5.8 or MS basic medium + NAA (α-naphthalene acetic acid) 0.15mg / L + banana juice 9% (V / V) + potato juice 6% (V / V )+taro juice 10%(V / V)+agar 5g / L, when the pH value was 5.5, also can reach the effect of embodiment 1. ...

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Abstract

The invention relates to a tissue culture method, in particular to a method for tissue culture of dendrobium candidum, which belongs to the technical field of biology and solves the problem that method for raising directly transplantable seedlings of dendrobium candidum needs to be provided. In order to solve the problem, an existing culture medium formula is modified, a special tissue culture cup with good light permeability, low infection rate and sufficient air is creatively provided, and a clean and appropriate natural light environment is provided. By means of the formula, the cup and the environment, capsule seeds of a plant of dendrobium candidum can be subjected to tissue culture for 7-8 months to breed 200,000 tissue culture seedlings of dendrobium candidum, the tissue culture seedlings can be directly transplanted outdoor with no need of traditional domestication (seedling exercising) for 3-5 months, and transplant survival rate reaches more than 98%.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a tissue culture method for dendrobium officinale, and belongs to the field of biotechnology. Background technique [0002] Many of the plants of the genus Dendrobium are famous and precious traditional Chinese medicines, and Dendrobium is listed as top grade in "Compendium of Materia Medica". Among the 76 species of Dendrobium plants in my country, nearly 40 are used for medicinal purposes. The famous ones are Dendrobium nobile, Dendrobium officinale, and Dendrobium Huoshanense. Functions are being tapped day by day, and its scope of application is expanding rapidly. At the same time, about one-fourth of Dendrobium can be used for viewing. It is famous for its gorgeous flowers. In recent years, it has developed rapidly as an ornamental plant and has formed an independent industry. In view of the value of both medicinal and ornamental, the excessive harvesting and utilization of it, co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 章亚辉章纪海
Owner YUEQING DINGYUAN BIOLOGICAL TECH
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