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Open type factory rapid propagation method for detoxification seedlings of potatoes

A technology of virus-free seedlings and potatoes, which is applied in horticultural methods, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of harsh environmental requirements in the tissue culture room, small number of cutting seedlings and tubers, and cumbersome operating procedures, etc. Achieve the effects of improving quality and survival rate, simple factory operation, and improving reproductive efficiency

Active Publication Date: 2018-06-15
内蒙古中薯联企业管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the harsh environmental requirements of the tissue culture room in the current process of cutting and growing seedlings of virus-free potato seedlings, it needs to be carried out under strict aseptic conditions, the operation procedures are cumbersome, low efficiency, high cost of cultivation, low survival rate of transplanting, and the number of tubers produced by cutting seedlings. In order to solve these problems, the technology of rapid propagation of virus-free potato seedlings has been systematically studied, and a new method of industrial rapid propagation of virus-free potato seedlings has been invented, which makes the rapid propagation of virus-free potato seedlings out of the strict aseptic operating environment. It is operated in a natural and open environment with bacteria, and at the same time, it simplifies the operation procedure of rapid propagation, which increases the propagation speed by more than 10 times compared with the traditional method, and greatly improves the quality and survival rate of virus-free potato seedlings. The production cost is lower than that of the traditional method. 1 / 6~1 / 7 of

Method used

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  • Open type factory rapid propagation method for detoxification seedlings of potatoes
  • Open type factory rapid propagation method for detoxification seedlings of potatoes
  • Open type factory rapid propagation method for detoxification seedlings of potatoes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Materials:

[0058] On March 24, 2015, 20,000 plantlets of Dutch fifteen-potato virus-free tissue culture seedlings were selected, with a plant height of 10-14 cm; the culture carrier was prepared, and the culture carrier was 20 bags of new vermiculite processed at high temperature; a clean greenhouse was prepared 1 During the cultivation period, the greenhouse is an intelligent greenhouse in Shebiya, Hohhot, Inner Mongolia. During the cultivation period, the temperature of the intelligent greenhouse is 20-25°C during the day, 18-22°C at night, and the humidity is 80-90%.

[0059] (2) Method:

[0060] (1) The seedbed is first covered with a layer of horticultural ground cloth;

[0061] (2) Spread the culture carrier vermiculite evenly on the off-ground seedbed of the greenhouse, with a thickness of 5-8 cm.

[0062] (3) Spray the culture carrier vermiculite with water to make it saturated with water, press your fingers to see the water, and it is better not to see ...

Embodiment 2

[0090] (1) Preparatory work:

[0091] On April 3, 2016, a clean greenhouse was prepared. The greenhouse was a thick-walled solar greenhouse in Shebiya Village, Hohhot, Inner Mongolia; 100,000 plantlets of detoxified Connebaker potato seedlings were prepared; the culture carrier was prepared. 100 bags of new vermiculite processed at high temperature; during the cultivation period, the greenhouse temperature is maintained at 21-25°C during the day, 18-21°C at night, and the humidity is 80-90%.

[0092] (2) Method:

[0093] (1) First spread a layer of horticultural ground cloth on the off-the-ground seedbed;

[0094] (2) Spread the culture carrier vermiculite evenly on the off-ground seedbed of the greenhouse, with a thickness of 5-8 cm.

[0095] (3) Spray the culture carrier vermiculite with water to make it saturated with water, press your fingers to see the water, and it is better not to see the water with the naked eye.

[0096] (4) Get the virus-free seedlings of the cult...

Embodiment 3

[0123] (1) Preparatory work:

[0124] At the beginning of March 2017, a clean greenhouse was prepared. The greenhouse was Wulanbulang solar greenhouse in Honghe Town, Qingshuihe, Hohhot, Inner Mongolia; prepared off-the-ground seedbeds; prepared 200,000 Xiapoti potato detoxified seedlings; prepared to cultivate Carrier, the carrier is 200 bags of new vermiculite processed at high temperature; during the cultivation period, the temperature of the greenhouse is kept at 21-25°C during the day, 18-21°C at night, and the humidity is 80-90%.

[0125] (2) Method:

[0126] (1) First spread a layer of horticultural ground cloth on the off-the-ground seedbed;

[0127] (2) Spread the culture carrier vermiculite evenly on the off-ground seedbed of the greenhouse, with a thickness of 5-8 cm.

[0128] (3) Spray the culture carrier vermiculite with water to make it saturated with water, press your fingers to see the water, and the standard is that no water can be seen with the naked eye. ...

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Abstract

The invention discloses an open type factory rapid propagation method for detoxification seedlings of potatoes. According to the method, no culture medium is needed, so that a process of high-pressuresterilization of the culture medium is eliminated; no bacteriostatic agent is needed, so that the influence of the bacteriostatic agent on growth of the detoxification seedlings is avoided; detoxifying tissue-culture seedlings are directly propagated under an open and bacteria-containing environment without a super-clean worktable; the rapidly propagated stem cutting speed per capita is 10 timesor above that of a conventional mode; the propagation coefficient is 3 to 4 times; the propagation time is shortened to 14 to 15 days; the detoxification seedlings of the potatoes, which are culturedin an open and bacteria-containing greenhouse, can grow robustly; after the detoxification seedlings enter a breeder's seed production stage, the survival rate can be up to 100 percent; the factory rapid propagation production cost is 1 / 6 to 1 / 7 of that of the conventional mode; furthermore, the tuberization number is obviously higher than that of conventional detoxification seedlings. The open type factory rapid propagation method for the detoxification seedlings of the potatoes provides an important technical support for effectively reducing the production cost of factory rapid propagation of the detoxification seedlings of the potatoes, improving the propagation efficiency, producing robust and high-quality detoxification seedlings, planting and producing breeder's seeds, and producingbreeder's seeds through aeroponic culture or soilless culture.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of virus-free potato seedlings, and in particular relates to an open factory rapid propagation method of virus-free potato seedlings. Background technique [0002] Potatoes originated in Peru, South America, and are widely cultivated all over the world. Potatoes were introduced into my country in the late Ming and early Qing Dynasties, and have been cultivated in my country for nearly 400 years. Potato is an important grain and vegetable crop in the world. It has short growth period, wide adaptability, high yield, wide application, rich nutrition, and is resistant to storage and transportation. However, due to the attack of the virus, the potato is generally degraded during the cultivation process, resulting in a sharp decline in the quality and yield of the potato. There are many viruses that harm potatoes. Potatoes are asexually propagated crops. Viruses accumulate in potato tubers a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曹春梅王晓娇逯春杏许飞胡嘉鑫乔宇李文刚胡晓惠
Owner 内蒙古中薯联企业管理有限公司
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