Dendrobium nobi tissue culturing and planting method

A technology of Dendrobium chinensis and a cultivation method, which is applied in the field of tissue culture production of medicinal Dendrobium chinensis, can solve the problems of consuming Dendrobium seedlings, low survival rate of Dendrobium, and inability to effectively expand the scale of planting area, and achieves shortening of tissue culture. Time and cost, good social and economic benefits, thermal insulation and fertility effects

Inactive Publication Date: 2009-01-07
CHISHUI TRUSKY TRADITIONAL CHINESE MEDICINE IND DEV
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Problems solved by technology

It is currently the largest base of Dendrobium nobile in China, but its planting scale and output are far from meeting the huge demand of the market
[0004] However, in terms of planting methods, most drug farmers continue to use the traditional method, using seedlings for division and cutting cultivation, which consumes a large amount of Dendrobium seedlings, resul

Method used

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Examples

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Example Embodiment

[0028] Examples:

[0029] 1. Tissue culture production technology of Dendrobium nobile:

[0030] After harvesting the mature capsules of the wild Dendrobium nobile origin, place them in a clean petri dish. After the natural cracking occurs, put the seeds in a clean beaker and seal them, and store them in the refrigerator.

[0031] A. Put the fresh unsplit fruits or stored seeds through the disinfection process, and sow them into N6 basic medium + NAA (α-naphthalene acetic acid) 0.3 mg / L + potato juice 20% (V / V) + agar 5g / In L medium, carry out seed germination culture, culture temperature 22~25℃, daily light for 10-12 hours, light intensity 1800~25001x, 15~20 days seed embryos start to germinate, 25~30 days seed embryos swell, Gradually germinate to form green protocorm;

[0032] B. Transfer the protocorm into N6 minimal medium + BA (6-benzyl adenine) 0.2mg / L + potato juice 20% (V / V) + agar 5g / L medium for protocorm proliferation and differentiation. After 25 days, it can be obse...

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Abstract

The present invention relates to a tissue culture production technology and a cultivation method of dendrobium nobile, which include the following steps: inserting seeds of dendrobium nobile into a culture medium of N6 + NAA 0.3mg/L + potato juice 20%(V/V) + agar-agar 5g/L to perform cultivation, at 22-25 DEG C, for 10-12 hours of average diurnal light, under 1800-2500lx intensity of illumination, for 15-30 days; transplanting the cultivated seeds to a culture medium of N6 + BA 0.2mg/L + potato juice 20%(V/V) + agar-agar 5g/L to perform cultivation for 25 days; transplanting the cultivated seeds to a culture medium of N6 + NAA 0.2mg/L + banana juice 10%(V/V) + potato juice 20%(V/V) + agar-agar 6g/L for two months to form big seedlings; planting the big seedlings in wood chips which mixed blue stones and decomposed organic fertilizers for hardening-seedling, which begins in every March for one year, and spraying water in time; and transplanting the hardened seedlings to open ground or greenhouse to perform cultivation with a transplanting medium of shale + moss and to fasten the seedlings by cable fasteners, under appropriate humiture and light irradiation, managing with GAP qualitative specification.

Description

1. Technical field [0001] The patent application of the present invention relates to the technical field of cultivation of traditional Chinese medicinal materials, in particular to a tissue culture production technology and cultivation method of medicinal Dendrobium nobile. 2. Background technology [0002] Dendrobium nobile is the first of the three major species of Dendrobium recorded in "Chinese Pharmacopoeia", and it has been recorded in "Shen Nong Herbal Classic" and "Compendium of Materia Medica". The stem of Dendrobium nobile is used as medicine, which has the functions of benefiting stomach and promoting body fluid, nourishing yin and clearing heat, relieving pain, clearing throat, improving eyesight, lowering blood fat, and blood pressure. The main raw material of products such as "Dendrobium Capsules". The "Mailuoning" series of products developed with Dendrobium as the main raw material has been designated by the state as an essential medicine for cardiovascular ...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G1/00A01C1/00A01D91/04
Inventor 彭贵湖廖方华袁明贵张万兴廖晓康
Owner CHISHUI TRUSKY TRADITIONAL CHINESE MEDICINE IND DEV
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