Extraction and purification method of bioactive polysaccharides in dendrobium huoshanense

A technology for bioactive polysaccharides and purification methods, applied in the field of purification and extraction of bioactive polysaccharides in Dendrobium Huoshanense, can solve problems such as shortening extraction time, achieve the effects of reducing energy consumption, avoiding organic solvent residues, and reducing production costs

Active Publication Date: 2012-03-28
JIUXIANZUN HUOSHAN DENDROBIUM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The present invention aims to provide a method for extracting and purifying biologically active polysaccharides in Dendrobium Huoshanense. The technical problem to be

Method used

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  • Extraction and purification method of bioactive polysaccharides in dendrobium huoshanense
  • Extraction and purification method of bioactive polysaccharides in dendrobium huoshanense
  • Extraction and purification method of bioactive polysaccharides in dendrobium huoshanense

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Embodiment 1

[0031] 1. Microwave extraction: Grind 30 g of Dendrobium Huoshanense into coarse powder, add 600 mL of water to the obtained coarse powder, extract at 2450 MHz frequency, power 500 W, and 60 °C microwave constant temperature for 30 min, extract 4 times, and combine 4 extracts;

[0032] 2. Concentration: Concentrate the extract obtained in step 1 to a relative density of 1.15 (60°C) at a vacuum of -0.07 to -0.08MPa and a temperature of ≤70°C to obtain a concentrate;

[0033] 3. Alcohol precipitation: add 95vt% (volume percentage concentration, the same below) ethanol to the concentrated solution obtained in step 2 to obtain a mixed solution, and the volume percent concentration of ethanol in the mixed solution is 80vt%. 4.3 g of precipitate was obtained by centrifugation under the revolution / minute speed;

[0034] 4. Membrane filtration: Add 100mL of 60°C water to the precipitate obtained in step 3 to dissolve the precipitate, pass the solution through an ultrafiltration membra...

Embodiment 2

[0041] 1. Microwave extraction: crush 30 g of Dendrobium Huoshanense into a coarse powder, add 450 mL of water to the obtained coarse powder, extract at 2450 MHz frequency, power 560 W, and 80 °C microwave constant temperature for 10 min, extract 4 times, and combine the extracts from 4 times;

[0042] 2. Concentration: Concentrate the extract obtained in step 1 to a relative density of 1.19 (60°C) under the conditions of a vacuum of -0.07 to -0.08MPa and a temperature of ≤70°C to obtain a concentrate;

[0043] 3. Alcohol precipitation: add 95vt% ethanol to the concentrated solution obtained in step 2 to obtain a mixed solution, the volume percent concentration of ethanol in the mixed solution is 80vt%, centrifuge at 4000 rpm after standing for 24 hours to obtain a precipitate 4.6g;

[0044] 4. Membrane filtration: add 10mL of 60°C water to the precipitate obtained in step 3 to dissolve the precipitate, pass the solution through an ultrafiltration membrane with a molecular wei...

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Abstract

The invention discloses an extraction and purification method of bioactive polysaccharides in dendrobium huoshanense. The method comprises the following steps: crushing the dendrobium huoshanense, then adding water, performing microwave constant-temperature extraction, and performing vacuum concentration on extract solution till the relative density is 110-1.30 so as to get concentrated solution;adding ethanol into the concentrated solution, standing, and further performing centrifugation to get precipitate; adding the water into the precipitate for dissolution, enabling the solution to passthrough an ultrafiltration membrane in the molecular weight of 100KD under the pressure of 0.35MPa, collecting trapped fluid, then enabling the trapped fluid to pass through the ultrafiltration membrane in the molecular weight of 300KD under the pressure of 0.35MPa, and collecting passing fluid; and performing the vacuum concentration on the collected passing fluid, and drying to get a polysaccharide extract from the dendrobium huoshanense. By adopting the method, the extraction time can be shorted, and the yield of a crude extract can be improved from 20% to about 35% under the same extraction times; and the yield of the polysaccharides from the dendrobium huoshanense after purification can be improved from about 5% to 10-15%, and the content of the polysaccharides from the dendrobium huoshanense can be improved from 40% to above 60%.

Description

1. Technical field [0001] The invention relates to a method for extracting and purifying biologically active polysaccharides, in particular to a method for extracting and purifying biologically active polysaccharides in Dendrobium huoshanense. 2. Background technology [0002] Dendrobium Huoshanense was first recorded in "Compendium of Materia Medica", and now it is a national endangered species protection plant. It has rare resources and high medicinal value. The main components of Dendrobium Huoshanense are polysaccharides, the polysaccharides of Dendrobium Huoshanense are in the α-pyran configuration, and the average molecular weight is 2.2×10 4 Da is composed of glucose, mannose and galactose in a molecular molar ratio of 31:10:8, and also contains a small amount of alkaloids and polyphenols. Studies have shown that polysaccharides are closely related to the medicinal effects of Dendrobium Huoshanense. [0003] Current status of research on the application of natural po...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 金海英王新生李俊张炜玲谢亚凤
Owner JIUXIANZUN HUOSHAN DENDROBIUM CO LTD
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