Preparation method of cordyceps militaris active polysaccharide

Abstract

The invention relates to cordyceps militaris active polysaccharide prepared by a method comprising the following steps: performing ultrafine grinding on cordyceps militaris sporophores, then extracting with boiling water, and collecting supernatant fluid; and concentrating the supernatant fluid, then adding ethanol for deposition, passing a deposited part through a DEAE-sepharose column, desalting a part of 0-0.5N, and then performing freeze-drying. The invention also relates to an application of a cordyceps militaris extract in preparation of an anti-tumor activity product.

Description

technical field [0001] The invention relates to the field of edible fungus extraction, in particular to a preparation method of Cordyceps militaris polysaccharide with anti-tumor activity. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, is a fungus of the genus Cordyceps in the family Ergotaceae. It has the effects of nourishing the lungs and kidneys, nourishing the essence, stopping bleeding and resolving phlegm. Modern research has found that it can delay aging, resist fatigue and hypoxia, regulate the immune system, resist hypoxia, increase myocardial nutrition and blood flow, etc. [0003] At present, there are more and more health care products of Cordyceps militaris on the market, among which the polysaccharide components achieve anti-tumor effects by improving the immune function of the body. Due to the single extraction method at present, there are few studies on the relationship between the chemical composition, chain structure...

AI Technical Summary

Problems solved by technology

Due to the single extraction method at present, there are few studies on the relationship between the chemical composition, chain structure, molecular size and anti-tumor effect of Cordyceps polysaccharides, and the obtained products have low polysaccharide content and unclear active ingredients. Therefore, Cordyceps militaris polysaccharides The relationship between extraction process and antitumor activity lacks regularity

[0004] At present, the separation and purification of fungal polysaccharides at home and abroad mostly adopts steps such as crushing, degreasing, water extraction, concentration, alcohol precipitation, centrifugation, freeze-drying, dissolution, deproteinization, decolorization, column purification and other steps. The process is complicated and organic solvents are required for degreasing. , Deproteinization, column purification using a molecular sieve gel (SephadexG-100 or G-75), separated samples need to be collected manually, experimental detection of polysaccharides (phenol-sulfuric acid method), long process route, low production efficiency, and product cost High, difficult to achieve mass production

[0005] CN1560085A discloses: a kind of technology that utilizes the leftovers of culture medium produced in Cordyceps artificial cultivation to extract Cordyceps polysaccharide, this method can turn waste into treasure, reduce the waste of resources, improve economic benefit, but used chloroform etc. Organic solvents, not conducive to food safety

[0006] CN102558264A discloses: the method for extracting the polysaccharide of Cordyceps militaris in Cordyceps militaris, but, use edible alcohol, acetone, ether, and the process flow is too long

[0007] CN101124988A discloses: a kind of method extracting refined Cordyceps militaris polysaccharide from Cordyceps militaris, in order to obtain refined Cordyceps militaris polysaccharide, needs to use papain to deproteinize, and the cost is high

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