Novel method for extracting seaweed intermediate by enzyme process

An enzymatic extraction and intermediate technology, which is applied in the direction of organic fertilizers, etc., can solve the problems that there is no report on the complete process research of the compound enzyme extraction method, and no industrialization has been found, and achieves the effect of efficient preparation method and improved product effect.

Active Publication Date: 2015-04-22
山东筱萤生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] After careful investigation by our side, although there are such literature reports in China, they are all at the level of theoretical research, and most of them are phased research results. Some companies have industrialized this technology, so this project has a demonstration role in the same industry and can bring huge economic benefits and industry influence

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  • Novel method for extracting seaweed intermediate by enzyme process
  • Novel method for extracting seaweed intermediate by enzyme process
  • Novel method for extracting seaweed intermediate by enzyme process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 A kind of novel enzymatic method extracts the method for seaweed intermediate, comprises the following steps:

[0039] (1) Weigh 500 parts of dried kelp, wash it with water and soak it to fully absorb water to 1500 parts;

[0040] (2) Weigh 100 parts of water, add 3 parts of phosphoric acid therein, and mix well to obtain a phosphoric acid solution;

[0041] (3) 1500 parts of kelp obtained in step (1) are added to the phosphoric acid solution in step (2) to obtain mixture A;

[0042] (4) Take by weighing 10 parts of compound acid enzyme and dissolve in 500 parts of water, mix uniformly and add in the mixture A obtained in step (3), mix uniformly to obtain mixture B;

[0043] The compound acid enzyme is a mixture of acid xylanase, acid lipase, acid pepsin and acid exonuclease, and its mass ratio is 3:2:2:1;

[0044] (5) Stir the mixture B for 15-90min at a temperature of 40°C;

[0045] (6) Dissolve 65 parts of potassium hydroxide and 15 parts of compound ...

Embodiment 2

[0050] Embodiment 2 A kind of novel enzymatic method extracts the method for seaweed intermediate, comprises the following steps:

[0051] (1) Take 150 parts of dried sea-tangle, wash it with water and soak it to fully absorb water to 1000 parts;

[0052] (2) 200 parts of water are weighed, 3.5 parts of acetic acid are added thereto, and mixed to obtain an acetic acid solution;

[0053] (3) 1000 parts of kelp obtained in step (1) are added to the acetic acid solution of step (2) to obtain mixture A;

[0054] (4) 6 parts of compound acid enzymes are taken and dissolved in 350 parts of water, mixed uniformly and added to the mixture A obtained in step (3), mixed uniformly to obtain mixture B;

[0055] The compound acid enzyme is a mixture of acid chitosanase, acid xylanase, acid lipase, acid pepsin and exonuclease, and its mass ratio is 3:3:2:1:1;

[0056] (5) Stir the mixture B for 20 minutes at a temperature of 40°C;

[0057] (6) Dissolve 10 parts of inorganic basic compoun...

Embodiment 3

[0063] Embodiment 3 A kind of novel enzymatic method extracts the method for seaweed intermediate, comprises the following steps:

[0064] (1) Weigh 400 parts of dried kelp, wash it with water and soak it to fully absorb water to 2000 parts;

[0065] (2) Take by weighing 480 parts of water, add 1.5 parts of sulfuric acid to it, mix well, get sulfuric acid solution;

[0066] (3) 2000 parts of kelp obtained in step (1) are added to the sulfuric acid solution in step (2) to obtain mixture A;

[0067] (4) Take by weighing 13 parts of compound acid enzymes and dissolve in 580 parts of water, mix uniformly and add in the mixture A obtained in step (3), mix uniformly to obtain mixture B;

[0068] Described composite acid enzyme is the mixture of acid carboxylesterase, acid dextrin hydrolase, acid lipase, elastase, chitinase and exonuclease, and its mass ratio is 4:2:3:3: 1:1;

[0069] (5) Stir the mixture B for 30 minutes at a temperature of 55°C;

[0070] (6) Dissolve 70 parts o...

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Abstract

The invention relates to a novel method for extracting a seaweed intermediate by an enzyme process, which comprises the following steps: sufficiently cleaning and immersing dry Laminaria digitata, adding into an acidic solution, and stirring uniformly to obtain a mixture A; dissolving a composite acidic enzyme in water, and adding into the mixture A to perform primary stirring extraction, thereby obtaining a mixture B; dissolving an inorganic alkaline compound and a composite alkaline enzyme in water, and adding into the mixture B to carry out secondary stirring extraction, thereby obtaining the mixture C; and dissolving a preserving agent in water, adding into the mixture C, and stirring uniformly to obtain the seaweed intermediate. The method for preparing the seaweed intermediate is efficient, quick, mild, comprehensive, safe and harmless. Compared with the traditional acid-alkali extraction technique, the extraction efficiency is enhanced by 50% above, the algal polysaccharides content is enhanced by 50-100%, the alginic acid content is 3.5-4.0, the effect of the seaweed intermediate product is obviously enhanced, and all the effective components in the seaweeds are reserved in the product, thereby implementing complete-effect addition in deed.

Description

technical field [0001] The invention relates to a novel enzymatic method for extracting seaweed intermediates, which belongs to the field of biological resource extraction. Background technique [0002] The ocean area accounts for 70% of the earth's surface. The seaweed that grows in seawater is a large family of marine biological resources. There are 250 genera and more than 1,500 species. Seaweed is rich in various nutrients, including vitamins, polysaccharides, alginic acid, and nectar. Alcohol, betaine, highly unsaturated fatty acids, antibiotics and a variety of natural plant hormones. Seaweed has a simple biological structure, which is conducive to processing and extracting active substances, and has been widely used in medicine, food, industry, agriculture and other fields. Seaweed fertilizer refers to the biological fertilizer produced from seaweed or seaweed extract through fermentation, acid-base process or fertilizer mixing process. The fertilizer has been clear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/10
Inventor 李昆仑赵林许士明张娟孙欣
Owner 山东筱萤生物科技有限公司
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