Method for extracting protein and polypeptide from walnut dregs

A protein polypeptide and walnut protein technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of low polypeptide interest rate, low protein extraction rate and protein purity, complex protein polypeptide preparation process, etc. The effect of simplifying the preparation process, enhancing human immunity, and shortening the extraction time

Active Publication Date: 2018-03-30
YUNNAN ACAD OF FORESTRY
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of low protein extraction rate and protein purity, serious emulsification, low polypeptide yield and complicated protein polypeptide preparation process in the existing walnut meal protein polypeptide preparation process, the present invention provides a method for extracting protein polypeptide from walnut meal method, can quickly extract and prepare protein polypeptide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting protein and polypeptide from walnut dregs
  • Method for extracting protein and polypeptide from walnut dregs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for extracting and preparing the walnut protein polypeptide powder of the present embodiment comprises the following steps:

[0026] Step (1), raw material pretreatment: crush the raw material of walnut meal with a pulverizer, and pass through a 60-mesh sieve to obtain crushed walnut meal, and then mix it with water at a weight ratio of 1:5 to obtain a walnut meal mixture, Add non-protein compound enzymes to the mixed solution, the non-protein compound enzymes are composed of cellulase, pectinase and amylase in a ratio of 5:2:3, and the amount of non-protein compound enzymes added is 0.1% of the quality of the walnut crushed material, The reaction temperature is 35°C, the reaction time is 3 hours, the whole process is stirred, and the stirring speed is 30r / min, and a suspension of walnut meal broken wall is obtained;

[0027] Step (2), alkali dissolution and enzymatic hydrolysis: add the above suspension to the reaction tank, and add Ca(OH) to the above suspe...

Embodiment 2

[0032] The preparation method for extracting walnut polypeptide powder of the present embodiment comprises the following steps:

[0033] Step (2), raw material pretreatment: crush the raw material of walnut meal with a pulverizer, and pass through a 60-mesh sieve to obtain crushed walnut meal, and then mix it with water at a weight ratio of 1:6 to obtain a walnut meal mixture, Add non-protein complex enzymes to the mixed solution, the non-protein complex enzymes are composed of cellulase, pectinase and amylase in a ratio of 4.5:2:3.5, and the amount of non-protein complex enzymes added is 0.2% of the walnut crushed material quality, The reaction temperature is 37°C, the reaction time is 4 hours, the whole process is stirred, and the stirring speed is 30r / min, and a suspension of walnut meal broken wall is obtained;

[0034]Step (2), alkali dissolution and enzymatic hydrolysis: add the above suspension to the reaction tank, add NaOH solution to the above suspension, adjust the ...

Embodiment 3

[0039] The preparation method for extracting walnut polypeptide powder of the present embodiment comprises the following steps:

[0040] Step (1), raw material pretreatment: crush the walnut meal raw material with a pulverizer, and pass through a 60-mesh sieve to obtain the walnut meal crushed material, and then mix it with water at a weight ratio of 1:7 to obtain a walnut meal mixture solution, add non-protein compound enzymes to the mixed liquid, the non-protein compound enzymes are composed of cellulase, pectinase and amylase in a ratio of 5:1.5:3.5, and the amount of non-protein compound enzymes added is 0.3% of the mass of walnut crushed material %, the enzymatic hydrolysis temperature is 40°C, the enzymatic hydrolysis time is 5h, and the whole process is stirred at a stirring speed of 30r / min to obtain a suspension of broken walnut meal;

[0041] Step (2), alkali dissolution and enzymatic hydrolysis: add the above suspension to the reaction tank, add NaOH solution to the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention relates to a method for extracting protein and polypeptide from walnut dregs. The method includes the following steps of 1, raw material pretreatment; 2, alkali dissolution and enzymolysis; 3, high temperature enzyme deactivation; 4, solid-liquid separation; 5, filtration and drying. The method is simple and economical in process, protein extraction and polypeptide preparation are conducted at the same time, which effectively shortens the time of protein extraction and polypeptide preparation, and the extraction efficiency of the protein and the conversion rate of the polypeptideare further improved; the productive process is environment-friendly, and the products have the functions of good antioxidant activity, blood pressure reduction and the like, and can be directly applied to the fields of functional food, medicine, cosmetics and the like; material sources are wide, the cost is low, waste can be changed into things of value, and the additional value of the walnut dregs is improved.

Description

technical field [0001] The invention relates to a method for extracting protein polypeptides, in particular to a method for extracting protein polypeptides from walnut dregs, belonging to the field of food processing. Background technique [0002] my country's walnut planting area and output rank first in the world, and Yunnan ranks first in the country. As of 2015, Yunnan Province has a walnut planting area of ​​42.3 million mu and an output of 850,000 tons, ranking first in the country in both output and area. With the walnut production getting higher and higher, at present, there are more and more domestic walnut oil extraction manufacturers, and a large amount of defatted walnut meal is produced accordingly. Walnut meal is often used as feed and fertilizer or discarded, and the waste discharge pollutes the environment, and its added value is low. Studies have shown that the protein content of walnut meal after deoiling by cold pressing is as high as 50%, which is a high...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34
CPCC07K1/14C07K1/34C12P21/06
Inventor 缪福俊宁德鲁王洋肖良俊马婷李勇杰耿树香陈海云吴涛贺娜张艳丽徐田
Owner YUNNAN ACAD OF FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap