Tuna fishbone collagen sourced zinc chelated collagen peptide, preparation method and application thereof

A technology of bone collagen and tuna, applied to the preparation method of peptides, food preparation, peptide/protein components, etc.

Active Publication Date: 2015-06-17
中食都庆(山东)生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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After searching, there is no report on the preparation of zinc chelated collagen peptides from tuna fish bone collagen

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  • Tuna fishbone collagen sourced zinc chelated collagen peptide, preparation method and application thereof
  • Tuna fishbone collagen sourced zinc chelated collagen peptide, preparation method and application thereof
  • Tuna fishbone collagen sourced zinc chelated collagen peptide, preparation method and application thereof

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Embodiment

[0023] 1) Pretreatment of tuna fish bones: Add 0.1 mol / L NaOH solution to tuna (skipjack) fish bones according to the ratio of solid to liquid 1 g:15 mL, soak at 4 °C for 5 h to remove non-collagen protein; the treated fish bones The bone was repeatedly washed with distilled water to pH 7.0, drained, and 0.5 mol / L EDTA solution (pH 7.4) was added according to the ratio of solid to liquid 1 g: 5 mL, soaked at 4°C for 3 days, and the EDTA solution was replaced once a day to remove Calcium in fish bones is dried and crushed to obtain decalcified fish bone powder.

[0024] 2) Preparation of tuna fish bone collagen: add 0.5 mol / L acetic acid solution to the decalcified fish bone powder according to the ratio of solid to liquid 1 g:3 mL, soak at 4 °C for 3 days, centrifuge at 12 000 g for 30 min, and take the supernatant solution, adding NaCl to a final solution concentration of 0.9 mol / L, standing still for 60 min, and centrifuging at 15 000 g for 30 min to obtain a precipitate, wh...

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Abstract

The invention relates to a preparation method for a zinc chelated collagen peptide of tuna fishbone collagen. The preparation method specifically comprises the following steps: using tuna fishbone as a raw material; adopting an acid process to extract collagen; performing enzymolysis on the collagens with pepsin and trypsin; adopting ultrafiltration, macroporous resin column chromatography, gel column chromatography and reversed-phase high-performance liquid chromatography to separate and purify the collagens to obtain the zinc chelated collagen peptide Gly-Lys-Thr-Gly-Trp-Pro-Gly (GKTGWPG), wherein the molecular weight of the zinc chelated collagen peptide detected by ESI-MS is 701.73 Da. The zinc chelated collagen peptide is high in absorption and utilization due to the unique chelating and transport mechanism, and meanwhile capable of supplementing polypeptides / amino acid and zinc, so that the zinc chelated collagen peptide is an ideal zinc-supplementing substance.

Description

technical field [0001] The invention relates to a zinc chelated collagen peptide derived from tuna fish bone collagen and a preparation method and application thereof, belonging to the technical field of deep processing of aquatic products. Background technique [0002] Collagen peptide is favored by more and more consumers because of its unique efficacy in the application of medicine, health care products, food and cosmetics, making it the focus of the food industry, health care product industry and daily chemical industry. Zinc is an essential trace element for the human body, and its deficiency can lead to diseases such as poor growth and development, mental retardation, and decreased immunity in children. Traditional zinc supplements (such as zinc gluconate and zinc citrate) have poor stability, low absorption and utilization rate, large gastrointestinal irritation, and are not easy to take for a long time. The chelate salt formed by zinc and metal chelating peptide c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/36C07K1/34C07K1/20C07K1/16A61K38/39A61P3/02A23L1/29A23L1/305A23L33/18
CPCC07K7/06C12P21/06
Inventor 谭洪亮王斌迟长凤邓尚贵孙坤来
Owner 中食都庆(山东)生物技术有限公司
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